101 results about "Rhodotorula marina" patented technology

The invention relates to ocean rhodotorula YS-185 bred with high yield of astaxanthin, which is obtained from ocean, wherein the rhodotorula is preserved in CCTCC of Wuhan, China with the preservation number of CCTCC NO: M207077; the invention also relates to a method for producing astaxanthin by fermenting and cultivating the ocean rhodotorula YS-185. The astaxanthin produced by the ocean rhodotorula YS-185 is natural astaxanthin and can be widely applied to high-quality cosmetics, food and feed additives.

The invention provides a method for fermenting a phodotorula benthica culture, which comprises the following steps of: (1) separating, screening and purifying phodotorula benthica strains; (2) performing liquid fermentation propagation and conversion on the purified phodotorula benthica strains; (3) performing solid aerobic fermentation on the phodotorula benthica strains subjected to the liquid fermentation propagation and conversion and performing solid anaerobic fermentated phodotorula benthica wall breakage; and (4) performing low-temperature drying. The method has the characteristics of simple production process, short period, low cost, stable product quality and the like. The product prepared by the method of the invention functions well in food appetite increasing, increasing of intestinal beneficial bacteria and antioxidation, and can be used for feeding livestock, poultry and aquatic fur-bearing animals as feedstuff or a feed additive.

A starch-adsorption fluidized-bed process for drying the solid marine rhodotorula features use of continuous stream plus deep aeration culture and starch-adsorption fluidized-bed. The resultant has high concentration of rhodotorula cells (5-10 billions/g), high output and high living cell percentage (30%). It can be used to raise prawn and marine crab with high survial rate, health level and raising efficiency.

The invention relates to a new method for preparing high-activity ocean rhodotorula glutinis powder. The method comprises the following steps: beer yeast is taken as a raw material and hydrolyzed by hydrolase to be a basal medium after filtering; after ocean rhodotorula glutinis is inoculated at deep fluid for aerobic culture, and an ultrafiltration membrane is utilized in time to concentrate fermentation liquor and collect rhodotorula glutinis thallus; and the concentrated rhodotorula glutinis is mixed evenly with excipient to form wet cenobium, and then the wet cenobium are transmitted to expansion drying equipment by a screw stem for instantaneous drying at low temperature to obtain the rhodotorula glutinis dry powder of which the rhodotorula glutinis thallus reaches 10-20 billion/g, and the viable yeast content reaches above 60%. The rhodotorula glutinis prepared by the preparation method of the invention has high growth speed, high density of yeast as per unit volume and short incubation time; the ultrafiltration membrane is adopted to concentrate culture solution, which has small damage to thallus and easily keeps high activity of the concentrated rhodotorula glutinis; granulation is not needed after the concentrated rhodotorula glutinis solution and the excipient are mixed; and the obtained wet cenobium can directly enter the expansion drying equipment to for instantaneous drying at low temperature, thus ensuring high activity ratio of the rhodotorula glutinis powder in the whole production technology process.

The invention discloses a composite stichopus japonicus growth promotion micro-ecological preparation. The micro-ecological preparation is characterized by comprising photosynthetic bacteria, bacillus subtilis, lactic acid bacteria and rhodotorula benthica, wherein the concentration ratio of the photosynthetic bacteria to the bacillus subtilis to the lactic acid bacteria to the rhodotorula benthica is (3-5.5):(3.5-5):(0.5-1.5):(1-1.5); the final concentration of both the photosynthetic bacteria and the bacillus subtilis is (0.5-1.5)*10<4>cfu/ml; and the final concentration of both the lactic acid bacteria and the rhodotorula benthica is (2-4)*10<3>cfu/ml. When in use, 7-10 days are used as one cycle, the four micro-ecological preparations with the above concentration ratio are diluted by 10-15 times by water, are mixed uniformly and then are uniformly spilled into culture water respectively, in the second day of the primary feeding operation, the preparations are spilled with the amount that is a half of the feeding amount of the first day, and the proper water temperature for feeding is 15DEG C to 28 DEG C. The micro-ecological preparation for the healthy culture of stichopus japonicus is easy to get, significant in effect and relatively low in cost.

The invention relates to artificial pellet feed for adult turbot. The artificial pellet feed is characterized by comprising white fish meal, red fish meal, whey protein, soybean protein concentrate, soybean meal hydrolysate small peptide, scallop powder, chicken meal, high protein flour, alpha-starch, L-alanyl-L-glutamine, choline chloride, sea fish oil, soybean lecithin, krill meal, ocean red yeast, photosynthetic bacteria, Schizochytrium limacinum powder, haematococcus pluvialis powder, multivitamins, composite mineral substances, Chinese herbal medicines and sodium alginate. According to the feed, a plurality of kinds of raw materials are added into the feed, so that the comprehensiveness of nutrition is guaranteed. During actual culture of the turbot, the pellet feed for the adult turbot has the advantages that the feed is good in feeding promoting performance, fishes like the feed, are full easily and do not fall ill easily, the survival rate is high, and the feed coefficient is low; the digestibility and the feed conversion ratio are high; the growth rate of the fishes is high, and the culture cycle is short.

The invention discloses a mandarin fish feed achieving high formability and growth promotion. The feed is prepared from fish meal, corn flour, gluten flour, tapioca flour, pumpkin powder, pine needle powder, potato powder, alpha-starch, dextrin, trash fishes, duck intestines, animal leather powder, palm oil, lecithin, bentonite, natural montmorillonite, allicin, olaquindox, saccharicterpenin, marine red yeast, gum tragacanth, guar gum, sodium alginate, seaweed gel, agar, sodium polyacrylate, carboxymethylcellulose, ligninsulfonate, urea formaldehyde condensates, polyvinyl alcohol, compound preservative, vitamin E, rare earth, a compound enzyme preparation, L-type tyrosine, phenylalanine, glycine betaine and trimethylamine hydrochloride. The mandarin fish feed is high in formability and suitable for feeding of mandarin fishes, the feeding environment of the mandarin fishes can be effectively improved, pollution to a water body is avoided after the feed is dispersed, mandarin fish production can be effectively promoted, the yield of the mandarin fishes is increased, and the quality of the mandarin fishes is improved.

The present invention discloses a medicinal bait additive for preventing and treating enterocytozoon hepatopenaei of litopenaeus vannamei. The medicinal bait additive comprises the following components in parts by weight: 8-32 parts of marine red yeast powder, 30-75 parts of albendazole, 8-32 parts of spirulina powder and 5-10 parts of garlic powder. The medicinal bait additive is used in a compound feed for the litopenaeus vannamei and has an addition amount of only 6-12%; and the medicinal bait additive can also be mixed with the feed at a weight ratio of 1:10 for feeding before feeding. Themedicinal bait additive uses an internal administration route, so that a medicine internal environment is formed in a host body, the medicinal bait additive is subjected to blood circulation to reachtarget organs and parasites cannot survive. The medicinal bait additive can effectively kill the enterocytozoon hepatopenaei in the litopenaeus vannamei body, is non-toxic to the litopenaeus vannameibody, does not cause medicine residues in the litopenaeus vannamei body, at the same time also does not cause pollution to the breeding environment, and can effectively play a therapeutic role in thediseased litopenaeus vannamei infected by the enterocytozoon hepatopenaei.

The invention relates to a method of producing an active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal. The method includes the steps of: 1) with the chrysanthemum meal, soybean meal, arginine concentrated bacterial slurry dried powder and corn steep liquor dry powder as raw materials and bacillus subtilis, arginine producing bacteria, rhodotorula benthica and lactobacillus plantarum as initial bacterial strains, respectively performing liquid seed cultivation and fermenting cultivation; 2) uniformly mixing a solid and a liquid according to ratio, placing the mixture on a fermentation bed and feeding sterilized air to perform aerobic solid fermentation cultivation; and 3) drying, crushing and sieving the product to obtain the active peptide powder for aquatic products, livestock and poultry. The active peptide powder has certain source of raw materials for fermentation, wherein the chrysanthemum meal, the arginine concentrated bacterial slurry dried powder and the corn syrup powder are low in cost and can produce a large amount of fermentation products. The active peptide powder is added to feeds for aquaculture and livestock and poultry breeding, thereby improving growth capability, improving product quality and also improving breeding environment. The active peptide powder is a green, environment-friendly and functional feed additive used for the aquatic products, livestock and poultry.

The invention provides a copper-enriching rhodotorula benthica strain WYN1 (Rhodotorula sp.WYN1) of high-yield carotenoid. The strain is preserved in China Center for Type Culture Collection on November 25th, 2014, and the preservation serial number is CCTCC No: M2014592. By the adoption of the rhodotorula benthica strain WYN1, high-yield carotenoid can be cultivated in a culture medium containing copper. A specific fermentation cultivation method comprises the steps that, preferably, the fermentation medium comprises 2.5% of glucose, 2.5% of peptone, 0.02% of magnesium sulfate, 0.15% of monopotassium phosphate, 300mg/L of copper, 20% of sodium chloride and the balance water; the initial pH value is 8, the inoculum size is 10%, the bed shaking rotating speed is 180 r/min, culturing is conducted for 24 hours at the temperature of 28 DEG C, the temperature is raised to 30 DEG C, the pH value is adjusted to 5, a nitrogen source is replenished once, namely 2.5% of peptone is added, and then fermentation is conducted for 24 hours.

The invention discloses a technology of using ocean phaffiarodozyma for producing feed additive astaxanthin in a fermentation mode. The technology includes the steps that glucose, yeast extract powder and the like serve as main raw materials to carry out liquid state submerged fermentation cultivation of special type yeast strains; fermenting-ripening astaxanthina saccharomycetes liquid is pumped into a horizontal low speed centrifuge to be centrifuged; astaxanthina saccharomycetes residue suspension liquid obtained through centrifugation is squeezed in a box pressure filter until the solid content is about 30%, the suspension liquid is moved into a fluidized bed and dried at low temperature till the moisture content is reduced to below 5%, and then the feed additive astaxanthin with the astaxanthin content of 10% is obtained through pulverizing and filtering. The technology has the advantages that as the astaxanthin production capacity strains are matched with a highly shared and complementary product extracting and purification technology, two most important natural antioxidant chiral drug intermediates and the feed additive are simultaneously obtained through one production line, the production cost is reduced, and the productivity benefits of the astaxanthin are increased.

The invention provides an environment-friendly pig feed additive product. The environment-friendly pig feed additive product comprises the following components in parts by weight: 8-15 parts of compound polysaccharide extract, 8-20 parts of an oceanic red yeast fermentative substance, 5-10 parts of epimedium extract, 3-8 parts of liquorice, 3-8 parts of hawthorn fruit, 1-5 parts of honeysuckle and 1-5 parts of dandelion, wherein the compound polysaccharide extract is obtained from astragalus, wolfberry and alfalfa, and the preservation number of oceanic red yeast is CCTCC NO:M2014592. The green pig feed additive product has the advantages that the oceanic red yeast can produce carotenoid with high yield and also can enrich copper, yield of carotenoid and copper content of the yeast are effectively improved by adopting a static cultivation method, the oceanic red yeast is applied to feed as a feed additive, can provide carotenoid, B-vitamins and copper ions, replaces inorganic copper ions used in the conventional feed due to high-efficiency copper enriching capability, is good in stability and can replace antibiotic to be used in feed through compatibility of Chinese herbal medicines, safety of a pork product is improved, organism immunity of a pig can be enhanced, intestinal health is regulated, metabolism function and fertility of the pig are improved, and feeding benefit is improved.

The invention provides a special compound feed for breeding parent prawns of penaeus monodon. The feed comprises the following components in percentage by weight: 25%-40% of fish meal, 5%-9.5% of artemia powder, 3%-6% of enzymolysis fish soluble pulp powder, 3%-9% of schizochytrium limacinum powder, 1%-3% of kelp powder, 2%-8% of hermetia illucens powder, 2%-5% of shrimp shell powder, 6%-12% of mussel powder, 2%-5% of marine red yeast dry powder, 10%-15% of flour, 1.5%-3% of lysolecithin, 0.8%-1.4% of ARA purified oil, 1.5% of DHA purified oil, 1.5% of EPA purified oil, 3.1%-3.7% of stearic acid triglyceride, 1%-2% of fish oil, 1%-5% of monocalcium phosphate, 0.9%-1.22% of vitamin premix and 1.08%-1.4% of mineral premix. According to the special compound feed, the growth performance of the parent prawns of penaeus monodon in the breeding period is remarkably improved, gonad development of the penaeus monodon is promoted, and the egg laying amount, the egg laying rate, the continuous egg laying rate, the fertilized egg hatching rate, the metamorphosis rate and the egg diameter of the parent prawns are increased.

The invention provides a feed additive product. The feed additive product is composed of the following components: 10%-15% of fructo-oligosaccharide, 10%-20% of propolis, 10%-25% of glossy privet fruit extract, 15%-30% of ocean rhodotorula fermentation culture and 30%-50% of ganoderma lucidum solid fermentation culture, wherein the preservation number of the ocean rhodotorula fermentation culture is CCTCC No:M 2014592, and the ratios are mass percent. According to the feed additive product, ocean rhodotorula can produce carotenoid with high yield and also can gather copper; the carotenoid yield and copper content of a yeast are effectively improved through a static cultivation method; the ocean rhodotorula is applied to a feed as a feed additive and can provide the carotenoid, group-B vitamins and copper ions; the ocean rhodotorula can replace inorganic copper ions used in conventional feed due to the efficient copper gathering capability; the product has good stability, good compatibility with other components in the feed, high absorption rate, low excretion rate and small environmental pollution, can be used for improving the skin complexions and hair colors of animals, and enhancing the nutritive value of the feed.

The invention discloses a method for producing carotenoid through fermentation. The method comprises the following steps: by taking yellow peach cannery trimmings as a main raw material, carrying out microwave fixation, frozen-breaking and biological enzymolysis to obtain fruit juice with relatively high carotenoid content, adjusting components to obtain a fermentation medium, inoculating oceanic red yeast producing the carotenoid with high yield into the fermentation medium, carrying out fractional inoculation and temperature shift fermentation so that maximization of proliferation of oceanic red yeast is realized, carrying out ultrahigh pressure homogenization, vacuum centrifugation and demulsification separation on fermentation broth to obtain emulsion, and extracting, separating and purifying the emulsion to obtain the carotenoid finished product, wherein the cell biomass of the fermentation broth is 38-52g/L, and the yield of the carotenoid is 47-61mg/L. The preparation method is simple, short in period, high in efficiency, energy-saving and environment-friendly, the whole course adopts a low temperature biologic processing technology, the production cost is reduced, the hidden environmental pollution danger is eliminated, and a solid foundation is laid for sustainable development of the technical field of deep processing of yellow peaches, so that the method disclosed by the invention has positive economic and social development significance.

The invention discloses a crab feed added with a marine red yeast extract. The crab feed comprises the following nutritional components in percentage by weight: 38-65% of protein source raw materials,3-11% of fat source raw materials, 20-35% of sugar source raw materials, 2% -6% of the marine red yeast extract, 1% -3% of fermented aloes, 0.4% -1.5% of compound vitamins and 1-1.5% of composite mineral salt. A preparation method of the marine red yeast extract comprises the following steps: taking marine red yeast dry powder, adding distilled water, 2-phenoxy ethanol and citric acid, performingultrasonic wall breaking, adding papain for enzymolysis, centrifuging, and carrying out freeze drying on supernate to obtain the marine red yeast extract. The preparation of the fermented aloes comprises the following steps: pretreating the aloes, wherein the extraction rates of vitamin, aloin and aloe polysaccharides can be improved by 4-Methyl heptanol and phenyl salicylate; preparing a fermentation strain; and preparing the fermented aloes. The feed has the beneficial effects that the feed is high in nutritive value, promotes the growth of crabs, and has the effect of improving the immunity.

The invention relates to a method for preparing a feed additive rich in astaxanthin through co-culture. The method comprises the following steps that firstly, a seed solution of phaffia rhodozyma is inoculated into a liquid fermentation culture medium, stirring fermentation is carried out, and a primary fermentation solution is obtained; secondly, a seed solution of rhodotorula benthica is directly inoculated into the primary fermentation solution, stirring fermentation is carried out, and a mixed fermentation solution is prepared; thirdly, the mixed fermentation solution is centrifuged for solid and liquid separation, the solid is taken and subjected to vacuum freezing drying, and the feed additive rich in astaxanthin is prepared. According to the method, rhodotorula benthica fermentation feed rich in astaxanthin is prepared through the method of co-fermentation culture of phaffia rhodozyma and rhodotorula benthica for the first time, and the functionality of the rhodotorula benthica feed is greatly improved; when the rhodotorula benthica feed is applied to marine aquaculture, the disease resistance of fish, shrimps, crabs and the like can be remarkably improved, the immunocompetence of culture objects can be enhanced, and the rhodotorula benthica feed is low in price and suitable for large popularization in aquaculture.

The invention relates to a microbial fermentation technology and a production technology of a special aquatic feed, in particular to a production method for producing an amino acid bioactive peptide by a microbial fermentation method. Milt paste, soybean meal, peanut meal and dried corn steep liquor powder are used as fermentation raw materials; bacillus subtilis, bacillus natto, saccharomyces cerevisiae, rhodotorula benthica and lactobacillus acidophilus are used as microbial fermentation communities; five microorganism liquid seeds respectively adopt seed cultivation of a shaking flask and fermentation cultivation of a fermentation tank. After solid and liquid are uniformly mixed according to a proportion, the mixture is placed on a fermentation bed and is subjected to aerobic solid fermentation by filling in sterile air; then by drying, grinding and sieving, the amino acid bioactive peptide for the aquatic products is obtained. The production method has the characteristics of wide raw material source, mature fermentation process, high protein content of the product, reasonable amino acid component, high peptide conversion rate, more enzyme products, good attractant effect and the like. When the amino acid bioactive peptide is applied to aquatic livestock such as marine and freshwater fishes, shrimps, crabs, spectacular fish stock and the like, diseases can be prevented and treated, a survival rate and an immunity are improved, a growth speed and quality are improved, the breeding environment also can be improved, and water quality is purified; the amino acid bioactive peptide for the aquatic products is a green functional feed additive.

The invention discloses a method for producing rhodotorula benthica by subsurface fermentation of high-density liquid. The method comprises the following steps of: firstly, inoculating the rhodotorula benthica into a YPD (Yeast Peptone Dextrose) liquid culture medium, carrying out shaking culture for 44-48 hours and taking the cultured liquid as a fermentation seed liquid; secondly, inoculating the fermentation seed liquid in the first step into a No.1 fermentation tank for stirring and carrying out aerobic fermentation culture for 16-20 hours; and thirdly, subcultivating the fermentation seed liquid in the No.1 fermentation tank to a No.2 fermentation tank at the volume ratio of 4-7.5 percent for stirring and carrying out aerobic fermentation culture for 8 hours, adding a carbon source and a nitrogen source at the uniform speed and culturing for 26-32 hours. According to the production method for culturing the rhodotorula benthica by adopting a culture medium formula and liquid subsurface ventilation and carbon and nitrogen source feeding, the fermentation time is shortened, the fermentation level of the rhodotorula benthica is greatly improved, the rhodotorula benthica is fermented and cultured in a 10 ton tank for 26-32 hours, the content of viable organisms in the fermented liquid reaches 50-70 hundred million/ml and the production benefit is effectively increased.

The invention relates to the field of aquatic feed, in particular to efficient ecological complete formula feed for culture of stichopus japonicus. The complete formula feed comprises ingredients and dry matters in percentage as follows: 20%-25% of sargassum pallidum powder, 12%-15% of sargassum thunbergii powder, 8%-10% of ulva pertusa powder, 2%-3% of spiral seaweed powder, 8%-12% of ophiopholis mirabilis powder, 2%-3% of whitefish powder, 4%-6% of scallop leftover powder, 2%-3% of shrimp powder, 3%-6% of soyabean protein powder, 1%-2% of oyster shell powder, 1%-2% of seashell powder, 2%-4% of marine red yeast, 0.3%-0.4% of multi-vitamins, 0.6%-0.7% of composite mineral salt, 15%-20% of sea mud powder and 4%-8% of yellow mud powder. The holothurian feed can supply comprehensive and balanced nutrition to cultured animals through scientific formula proportioning, and other nutritional substances are not required to be supplemented during use; the feed has high feeding attraction, good palatability and high utilization ratio and can remarkably increase the growth rate and the husk ratio during culture of stichopus japonicus. The feed is high-quality complete artificial formula feed for holothurian culture.

The invention discloses a preparation method of a fermentation type chicken feed. The method comprises the following steps: (1) evenly mixing strains, wherein the strains comprise aspergillus niger, geotrichum candidum and trichoderma; (2) evenly mixing the mixed strain with main ingredients, adding nutritive salt and stirring to obtain a mixed feed, wherein the main ingredients comprise bean pulp and bran, and the nutritive salt comprises urea, ammonium sulfate, calcium superphosphate, bentonite, salt and water; (3) putting the mixed feed into a fermenting room for fermenting, and adding the fermented mixed feed into coarse food grain in proportion to obtain a fermented feed; (4) breaking the cell wall of oceanic red yeast to prepare an oceanic red yeast culture solution, adding the prepared oceanic red yeast culture solution into the fermented feed, evenly mixing, and then drying to obtain the final chicken feed. The prepared chicken feed contains coarse food grain protein, mycoprotein, protein in the oceanic red yeast, carotenoid and the like; by promotion and coordination of nutrient elements, a powerful guarantee is provided for the growth of chai chicken, and nutrition types which can be obtained by the chai chickens are enriched.