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Copper-enriching rhodotorula benthica strain of high-yield carotenoid and fermentation cultivation method thereof

A technology of marine red yeast and carotene, which is applied in the directions of microorganism-based methods, fermentation, biochemical equipment and methods, etc., to achieve the effects of low excretion rate, good compatibility and low environmental pollution

Active Publication Date: 2015-09-23
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no relevant report on the marine rhodotorula that can produce high carotenoids and can also enrich copper. The present invention expects to separate and obtain copper-rich marine rhodotorula with high carotene production from the marine environment, and determine it through single factor experiments and orthogonal experiments. It is suitable for the optimal culture conditions of marine red yeast carotenoids, provides a theoretical basis for industrial production, and explores a new way for the utilization of marine red yeast

Method used

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  • Copper-enriching rhodotorula benthica strain of high-yield carotenoid and fermentation cultivation method thereof
  • Copper-enriching rhodotorula benthica strain of high-yield carotenoid and fermentation cultivation method thereof
  • Copper-enriching rhodotorula benthica strain of high-yield carotenoid and fermentation cultivation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance

[0018] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and culture. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator.

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Embodiment 2

[0035] Example 2: Research on Fermentation Conditions of High-yielding Carotenoids of Rhodotorula marina WYN1

[0036] The carbon source (including glucose, lactose, sucrose, maltose and starch), nitrogen source (including yeast powder, peptone, ammonium sulfate, potassium nitrate, ammonium nitrate and urea), fermentation time, pH value and metal ion ( Effects of factors such as copper ions, magnesium ions, zinc ions, ferrous ions, etc.) on the production of carotenoids in the marine Rhodotorula yeast WYN1.

[0037] (1) Effects of different carbon sources on carotenoid production of Rhodotorula marina WYN1

[0038] Glucose, sucrose, maltose, starch and lactose were selected as different carbon sources for fermentation experiments, and the addition amount was 2%. The experimental results are shown in Table 1.

[0039] Table 1: Effects of different carbon sources on carotenoid production of Rhodotorula marina WYN1

[0040]

[0041] It can be seen from Table 1 that glucose i...

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Abstract

The invention provides a copper-enriching rhodotorula benthica strain WYN1 (Rhodotorula sp.WYN1) of high-yield carotenoid. The strain is preserved in China Center for Type Culture Collection on November 25th, 2014, and the preservation serial number is CCTCC No: M2014592. By the adoption of the rhodotorula benthica strain WYN1, high-yield carotenoid can be cultivated in a culture medium containing copper. A specific fermentation cultivation method comprises the steps that, preferably, the fermentation medium comprises 2.5% of glucose, 2.5% of peptone, 0.02% of magnesium sulfate, 0.15% of monopotassium phosphate, 300mg / L of copper, 20% of sodium chloride and the balance water; the initial pH value is 8, the inoculum size is 10%, the bed shaking rotating speed is 180 r / min, culturing is conducted for 24 hours at the temperature of 28 DEG C, the temperature is raised to 30 DEG C, the pH value is adjusted to 5, a nitrogen source is replenished once, namely 2.5% of peptone is added, and then fermentation is conducted for 24 hours.

Description

technical field [0001] The invention relates to a marine rhodotorula strain capable of accumulating carotenoids and enriching copper and a fermentation culture method conducive to carotenoid accumulation, which belongs to the technical field of fermentation and comprehensive utilization of marine microorganisms. Background technique [0002] Carotenoids are a class of polyene compounds that are yellow, orange or red. They are the most ubiquitous and stable natural pigments in nature and widely exist in many animals and plants. As an excellent food additive and nutritional supplement for improving human nutrition, carotenoid has been recognized by countries all over the world for its excellent quality and effect, and is known as the most promising antioxidant. At present, carotenoids are widely used in industries such as food, medicine, cosmetics and feed additives. Due to their good application prospects and excellent functions, they have been favored by people for a long ti...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P23/00C12R1/645
CPCC12P23/00C12N1/145C12R2001/645
Inventor 王亚楠程殿林丛培江孙梦洁涂开杨婷婷刘敏
Owner QINGDAO UNIV
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