Method for preparing feed additive rich in astaxanthin through co-culture

A technology of feed additive and symbiotic culture, applied in the field of symbiotic culture to prepare astaxanthin-rich feed additive, and symbiotic culture to prepare astaxanthin-rich marine red yeast fermented feed additive, which can solve the problem of affecting the function of marine red yeast fermented feed Sex, low astaxanthin content, cost increase and other issues, to achieve the effect of enhancing immunity, cheap price, and improving functionality

Active Publication Date: 2016-06-01
SHANDONG BAILONG CHUANGYUAN BIO TECH
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AI Technical Summary

Problems solved by technology

[0003] The common problem of the marine red yeast feed produced by the traditional production process is that the content of astaxanthin is low, which greatly affects the functionality of the marine red yeast fermented feed. How to produce a low-cost marine red yeast rich in astaxanthin Yeast fermented feed has become an urgent problem to be solved
[0006] Among the above products, the content of astaxanthin is still low, and the cultivation of Phaffia rhodozyme is under harsh conditions, which leads to a significant increase in the cost of preparing astaxanthin with Phaffia rhodozyme

Method used

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  • Method for preparing feed additive rich in astaxanthin through co-culture

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Embodiment 1

[0045]A method for preparing a feed additive rich in astaxanthin through symbiotic culture, the steps are as follows:

[0046] (1) will contain 2.5×10 8 The seed liquid of Phaffia rhodozyme per ml was inoculated in the liquid fermentation medium according to the volume percentage of 5%. Under the conditions of temperature 25°C, ventilation rate 1.0vvm, and pH 4.5, stir and ferment, and add carbon source during fermentation solution and nitrogen source solution, so that the carbon source concentration in the fermenter is 10g / L, the nitrogen source concentration is 0.8g / L, ferment for 30h, and obtain a fermented liquid;

[0047] The components per liter of the liquid fermentation medium are as follows:

[0048] Sucrose 20g, glucose 10g, corn flour 5g, yeast extract 8g, soybean meal 10g, dipotassium hydrogen phosphate 1.5g, ammonium chloride 5g, anhydrous magnesium sulfate 5g, water to 1L, pH5.2;

[0049] (2) directly inoculate the primary fermented liquid that step (1) makes c...

Embodiment 2

[0074] A method for preparing a feed additive rich in astaxanthin through symbiotic culture, the steps are as follows:

[0075] (1) will contain 2.0×10 8 The seed liquid of Phaffia rhodozyme per ml was inoculated in the liquid fermentation medium according to the volume percentage of 6%, under the conditions of temperature 26°C, ventilation rate 1.2vvm, pH 4.8, stirred and fermented, and carbon source was added during the fermentation solution and nitrogen source solution, so that the carbon source concentration in the fermenter is 15g / L, the nitrogen source concentration is 1.0g / L, ferment for 35h, and obtain a fermented liquid;

[0076] The components per liter of the liquid fermentation medium are as follows:

[0077] Sucrose 25g, glucose 15g, corn flour 8g, yeast extract 10g, soybean meal 6g, dipotassium hydrogen phosphate 1g, ammonium chloride 3g, anhydrous magnesium sulfate 3g, water to 1L, pH5.3;

[0078] (2) directly inoculate the primary fermented liquid that step (...

Embodiment 3

[0103] A method for preparing a feed additive rich in astaxanthin through symbiotic culture, the steps are as follows:

[0104] (1) will contain 1.5×10 8 The seed liquid of Phaffia rhodozyme per ml was inoculated in the liquid fermentation medium according to the volume percentage of 10%. Under the conditions of temperature 26°C, ventilation rate 1.2vvm, pH 4.8, stir and ferment, and add carbon source during the fermentation solution and nitrogen source solution, so that the carbon source concentration in the fermenter is 15g / L, the nitrogen source concentration is 1.0g / L, ferment for 35h, and obtain a fermented liquid;

[0105] The components per liter of the liquid fermentation medium are as follows:

[0106] Sucrose 30g, glucose 15g, corn flour 10g, yeast extract 15g, soybean meal 5g, dipotassium hydrogen phosphate 0.5g, ammonium chloride 5g, anhydrous magnesium sulfate 5g, water to 1L, pH5.4;

[0107] (2) Directly inoculate the primary fermentation broth prepared in step...

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Abstract

The invention relates to a method for preparing a feed additive rich in astaxanthin through co-culture. The method comprises the following steps that firstly, a seed solution of phaffia rhodozyma is inoculated into a liquid fermentation culture medium, stirring fermentation is carried out, and a primary fermentation solution is obtained; secondly, a seed solution of rhodotorula benthica is directly inoculated into the primary fermentation solution, stirring fermentation is carried out, and a mixed fermentation solution is prepared; thirdly, the mixed fermentation solution is centrifuged for solid and liquid separation, the solid is taken and subjected to vacuum freezing drying, and the feed additive rich in astaxanthin is prepared. According to the method, rhodotorula benthica fermentation feed rich in astaxanthin is prepared through the method of co-fermentation culture of phaffia rhodozyma and rhodotorula benthica for the first time, and the functionality of the rhodotorula benthica feed is greatly improved; when the rhodotorula benthica feed is applied to marine aquaculture, the disease resistance of fish, shrimps, crabs and the like can be remarkably improved, the immunocompetence of culture objects can be enhanced, and the rhodotorula benthica feed is low in price and suitable for large popularization in aquaculture.

Description

technical field [0001] The invention relates to a method for preparing a feed additive rich in astaxanthin through symbiotic culture, in particular to a method for preparing a fermented feed additive rich in astaxanthin by marine rhodotorula, belonging to the technical field of biological feed. Background technique [0002] Marine red yeast refers to the yeast existing in natural sea areas, and its cells are rich in biologically active substances such as protein, amino acid, glycogen particles, unsaturated fatty acids, carotenoids, astaxanthin and various unknown nutritional factors. With the vigorous development of the aquaculture industry, marine red yeast has been widely selected as fresh bait for the larvae of sea cucumbers, shrimps, crabs, and shellfish in the planktonic stage. A large part of the function of marine red yeast is related to the astaxanthin it contains. Astaxanthin is a carotenoid with strong antioxidant and free radical elimination effects. It is known a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K20/105A23K50/80C12N1/16C12R1/645
CPCC12N1/16Y02A40/818
Inventor 李方华窦光鹏窦宝德干昭波邵先豹
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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