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Method for producing rhodotorula benthica by subsurface fermentation of high-density liquid

A technology of marine red yeast and high-density liquid, which is applied in the field of microbial fermentation, can solve the problems of shortening fermentation time, increasing the number of viable bacteria in fermentation liquid, and long fermentation time, and achieves the advantages of shortening fermentation time, improving production efficiency and improving fermentation level. Effect

Active Publication Date: 2012-09-19
福建大北农华有水产科技集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The marine rhodotorula used for production in the current reports has a low fermentation yield and a long fermentation time. It is necessary to adopt a reasonable medium formula and fermentation method to significantly increase the number of viable bacteria in the fermentation broth and shorten the fermentation time during the scale-up fermentation production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The process for producing Rhodotorula viscosus by high-density fermentation consists of the following steps:

[0025] Step 1. Inoculate Rhodotorula viscosus into 150ml of sterilized YPD liquid medium, and culture it in a shaker flask at 28°C and 150rpm / min for 48 hours as a fermentation seed solution.

[0026] Step 2. Inoculate 0.2% of the inoculum into a 1-ton tank with a liquid volume of 60%. The culture temperature is 28°C, the stirring speed is 90rpm / min, and the ventilation rate is 0.4m 3 / min for 20 hours. Among them, the medium formula in 1 ton tank is: molasses 18 kg, peptone 1.5 kg, yeast extract 1.5 kg, ammonium sulfate 1.5 kg, potassium dihydrogen phosphate 0.75 kg, magnesium sulfate monohydrate 0.06 kg, ferrous sulfate 0.075 kg, chloride 7.5 kg of sodium, adjust the pH to 5.5 with lye, and steam sterilize at 121°C for 50 minutes.

[0027] Step 3. Transfer 480L of fermentation liquid in a 1-ton fermenter to a 10-ton tank for fermentation and culture for 8 h...

Embodiment 2

[0033] The process for producing Rhodotorula viscosus by high-density fermentation consists of the following steps:

[0034] Step 1. Inoculate Rhodotorula viscosus into 150ml of sterilized YPD liquid medium, and culture it in a shaker flask at 28°C and 150rpm / min for 48 hours as a fermentation seed solution.

[0035] Step 2. Inoculate 0.1% of the inoculum into a 1-ton tank with a liquid content of 60%. The culture temperature is 25°C, the stirring speed is 80rpm / min, and the ventilation rate is 0.3m 3 / min for 16 hours. Among them, the medium formula in 1 ton tank is: molasses 12 kg, peptone 0.9 kg, yeast extract 0.9 kg, ammonium sulfate 1.5 kg, potassium dihydrogen phosphate 0.6 kg, magnesium sulfate monohydrate 0.03 kg, ferrous sulfate 0.06 kg, chloride 6 kg of sodium, adjust the pH to 5.5 with lye, and steam sterilize at 121°C for 50 minutes.

[0036] Step 3. Transfer 320L of fermentation liquid in a 1-ton fermenter to a 10-ton tank for fermentation and culture for 8 hour...

Embodiment 3

[0042] The process for producing Rhodotorula viscosus by high-density fermentation consists of the following steps:

[0043] Step 1. Inoculate Rhodotorula viscosus into 150ml of sterilized YPD liquid medium, and culture it in a shaker flask at 28°C and 150rpm / min for 44 hours as a fermentation seed solution.

[0044] Step 2. Inoculate 0.3% of the inoculum into a 1-ton fermenter with a liquid filling capacity of 60%. The culture temperature is 30°C, the stirring speed is 100rpm / min, and the ventilation rate is 0.5m 3 / min for 18 hours. Among them, the medium formula in 1 ton of fermenter is: molasses 15 kg, peptone 1.2 kg, yeast extract 1.2 kg, ammonium sulfate 3.0 kg, potassium dihydrogen phosphate 0.9 kg, magnesium sulfate monohydrate 0.09 kg, ferrous sulfate 0.09 kg, chlorine 9 kg of NaCl, adjust the pH to 6.0 with lye, and steam sterilize at 121°C for 50 minutes.

[0045] Step 3. Transfer 600L of fermentation broth in a 1-ton fermenter to a 10-ton tank for fermentation and...

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Abstract

The invention discloses a method for producing rhodotorula benthica by subsurface fermentation of high-density liquid. The method comprises the following steps of: firstly, inoculating the rhodotorula benthica into a YPD (Yeast Peptone Dextrose) liquid culture medium, carrying out shaking culture for 44-48 hours and taking the cultured liquid as a fermentation seed liquid; secondly, inoculating the fermentation seed liquid in the first step into a No.1 fermentation tank for stirring and carrying out aerobic fermentation culture for 16-20 hours; and thirdly, subcultivating the fermentation seed liquid in the No.1 fermentation tank to a No.2 fermentation tank at the volume ratio of 4-7.5 percent for stirring and carrying out aerobic fermentation culture for 8 hours, adding a carbon source and a nitrogen source at the uniform speed and culturing for 26-32 hours. According to the production method for culturing the rhodotorula benthica by adopting a culture medium formula and liquid subsurface ventilation and carbon and nitrogen source feeding, the fermentation time is shortened, the fermentation level of the rhodotorula benthica is greatly improved, the rhodotorula benthica is fermented and cultured in a 10 ton tank for 26-32 hours, the content of viable organisms in the fermented liquid reaches 50-70 hundred million / ml and the production benefit is effectively increased.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing marine red yeast by submerged fermentation of high-density liquid. Background technique [0002] Marine Rhodotorula is a single-celled eukaryotic organism that naturally exists in the ocean. The bacteria are round or oval, and the colonies are bright red. It is rich in protein, polysaccharides, unsaturated fatty acids, vitamins, digestive enzymes and Animal larval growth hormone and astaxanthin-based carotenoids and other nutrients are ideal biological bait for aquatic animal larvae. In addition, marine red yeast is widely present on the surface and digestive tract of marine fish, crabs, shrimps and other aquatic animals, and plays an important role in the health of marine organisms. [0003] CN1146290A (Application No.: 95112038.7) patent document discloses a method of separating marine red yeast from nature, carrying out a feeding liquid...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12R1/645
Inventor 马相杰刘雪连潘春丽李宁张玳华邓微微赵雅楠
Owner 福建大北农华有水产科技集团有限公司
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