Material supplementing method for reducing viscosity of gentamicin C1a fermentation liquid

A technology of gentamicin and fermentation liquid, which is applied in the field of fermentation engineering, can solve the problems of not being able to relieve glucose repression, and achieve the effects of improving the mixing transfer effect, simple and effective process, and reducing viscosity

Inactive Publication Date: 2017-04-26
HENAN RENHUA BIOTECH CO LTD
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AI Technical Summary

Problems solved by technology

For example, conventional carbon supplementation uses a single component of glucose. In the literature reports on the carbon metabolism of gentamicin, high concentrations of glucose have a strong inhibitory effect on the synthesis of gentamicin, but although glucose has an inhibitory effect on the synthesis of antibiotics, But at the

Method used

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  • Material supplementing method for reducing viscosity of gentamicin C1a fermentation liquid
  • Material supplementing method for reducing viscosity of gentamicin C1a fermentation liquid
  • Material supplementing method for reducing viscosity of gentamicin C1a fermentation liquid

Examples

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Example Embodiment

[0025] Example 1

[0026] Primary seed culture: at 2m 3 The seed tank is fed according to the composition ratio in Table 1. Sterilize the first-level seed culture medium, cool it, use sterile air to maintain the pressure, and then inoculate the 1L cultured shake flask seed liquid into the first-level seed tank for cultivation with a differential pressure gauge. The tank pressure is 0.04~0.08Mpa during the process. ; Tank temperature 33~38℃; air flow rate 0.7:1; rotation speed is 150rpm; pH value 6.0-8.0; cultivation time 45h. The concentration of the cultured first-level seed liquid is 21%, the pH is 7.0, and there is no contamination by bacteria.

[0027] Secondary seed culture: at 15m 3 Feed the medium according to the composition ratio in Table 1. Sterilize the secondary seed culture medium first, cool it, use sterile air to maintain the pressure, and then transfer all the cultured primary seed liquid into the secondary seed tube for cultivation. During the process, tank pres...

Example Embodiment

[0037] Example 2

[0038] The first-level seed culture, the second-level seed culture and the fermentation culture process are the same as in Example 1. In the feed control stage, the carbon sources used for the carbon supplement are: glucose 40%, maltose 5%, and D-xylose 2.0%.

[0039] The nitrogen sources used for nitrogen supplementation are: 15% urea, 20% ammonium sulfate, 1.5% corn steep liquor and 1% yeast extract powder.

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Abstract

The invention relates to a material supplementing method for reducing viscosity of a gentamicin C1a fermentation liquid, and belongs to the field of fermentation engineering, in the whole fermentation process only control of supplementing of two materials of carbon and nitrogen is performed in the method, reducing sugar concentration in a fermentation pot is controlled in 1-20g / L, when the reducing sugar concentration is lower than 5g / L, a continuous flow adding method is used for supplementing the carbon, when the reducing sugar concentration is higher than 20g / L, the supplementing of the carbon is stopped, the rate for the supplementing of the carbon is adjusted according to real-time off-line determined reducing sugar concentration and fermentation liquid viscosity; amino nitrogen in the fermentation pot is controlled in 20-60mg / mL, when the amino nitrogen concentration is lower than 30mg / mL, the continuous flow adding method is used for supplementing the nitrogen, when the amino nitrogen concentration is higher than 60mg / mL, the supplementing of the nitrogen is stopped, and the rate for the supplementing of the nitrogen is adjusted according to real-time off-line determined amino nitrogen concentration and fermentation liquid viscosity. The method is simple in process, can obviously reduce the viscosity of the fermentation liquid and improve the oxygen transfer, and has an important effect on increasing of the yield of gentamicin.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular relates to the fermentation production of gentamicin, in particular to a feeding method for reducing the viscosity of gentamicin C1a fermentation liquid. Background technique [0002] As an important aminoglycoside antibiotic, gentamicin C1a is the mother nucleus of a semi-synthetic new drug ictimicin, which is the first in China and has independent intellectual property rights. The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure and similar physical and chemical properties of each component, the extraction cost remains high, and the high cost in the market The price of purity C1a remains high. [0003] Due to the many multi-component gentamicin fermentation processes, the current single-component gentamicin C1a fermentation process mainly draws o...

Claims

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Application Information

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IPC IPC(8): C12P19/48
Inventor 程国政
Owner HENAN RENHUA BIOTECH CO LTD
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