Fermentation technology for industrially producing L-hydroxyproline through fermentation method

A technology of hydroxyproline and fermentation technology, applied in the field of microbial fermentation technology, can solve the problems of L-hydroxyproline fermentation level, production cost three waste discharge and the like, and achieves the advantages of reducing production cost, avoiding three waste pollution and improving fermentation level. Effect

Inactive Publication Date: 2016-04-20
TIANJIN JINGYE FINE CHEM
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the production of L-hydroxyproline by microbial fermentation is still in the laboratory stage, and a mature industrial production process has not yet been formed. However, the advanced industrial production process, especially the fermentation process, has a great impact on the fermentation level of L-hydroxyproline, Production cost and three waste emissions have a crucial impact

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0017] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0018] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0, Z...

example 2

[0023] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in a liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0024] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0025] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0,...

example 3

[0030] The fermentation process of industrial production of L-hydroxyproline by fermentation method, firstly, the strains are cultivated in a liquid medium to obtain mature seeds, and then transferred to the fermentation medium to ferment and metabolize to produce L-hydroxyproline;

[0031] Among them, the liquid seed medium aqueous solution formula (g / L) is: glucose 40, yeast powder 7.5, ammonium sulfate 2.0, potassium dihydrogen phosphate 7.5, potassium chloride 1.9, FeSO 4 ·7H 2 O0.28, MgSO 4 ·7H 2 O0.32, trace elements (ppm): MnSO 4 ·H 2 O0.5, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.2, CuSO 4 ·7H 2 O0.5, ZnSO 4 ·7H 2 O0.6, VB120, defoamer 100, AMP50.

[0032] The fermentation medium formula (g / L) is as follows: glucose 20, yeast powder 5.0, ammonium sulfate 10, potassium dihydrogen phosphate 5.0, potassium chloride 1.9, MgSO 4 ·7H 2 O0.32, FeSO 4 ·7H 2 O0.75, trace elements (ppm): MnSO 4 ·H 2 O1.0, CoCl 2 ·6H 2 O1.4, NiSO 4 ·6H 2 O1.4, CuSO 4 ·7H 2 O1.0,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
Login to view more

Abstract

Provided is a fermentation technology for industrially producing L-hydroxyproline through a fermentation method. The fermentation technology comprises the following steps that 1, a strain is cultured in a liquid culture medium, and a mature seed is obtained; 2, the mature seed is moved into the fermentation culture medium in a fermentation tank for fermentation and metabolism, and the L-hydroxyproline is produced. The fermentation technology is applied to production of the L-hydroxyproline through microbial fermentation, the fermentation level is improved, the production cost is reduced, and three-waste pollution is avoided.

Description

technical field [0001] The invention relates to a microbial fermentation process, in particular to a fermentation process for the industrial production of L-hydroxyproline by a fermentation method. Background technique [0002] Hydroxyproline (Hyp) is an imino acid, which is the product of L-proline hydroxylation, and its molecular formula is C 5 h 9 NO 3 . Hydroxyproline is white flaky crystal or crystalline powder, slightly sweet, with a melting point of 274°C, easily soluble in water and slightly soluble in ethanol. Trans-4-hydroxyproline is more common in nature, and trans-4-hydroxyproline was first found in animal collagen. Trans-4-hydroxyproline is widely used in medicine, chemical industry, animal feed, nutrition and beauty industry, etc. [0003] At present, the methods for producing hydroxyproline reported at home and abroad mainly include proteolytic extraction, chemical synthesis, microbial fermentation and enzymatic conversion. Among them, proteolytic extrac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/24
CPCC12P13/24
Inventor 林俊辉赵体金彭久合
Owner TIANJIN JINGYE FINE CHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap