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Method for producing carotenoid through fermentation

A technology of carotene and fermentation medium, which is applied in the field of fermentation and production of carotenoids, to achieve the effects of preventing bacterial contamination, realizing high yield, improving proliferation ability and survival rate of viable bacteria

Inactive Publication Date: 2016-07-20
BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem solved by the present invention is to overcome the defects of the existing fermentation preparation of carotenoids, and use the leftovers of canned yellow peaches as the main raw material to obtain fruit juice with high carotenoid content through microwave solidification, freezing and crushing, and biological enzymatic hydrolysis , after adjusting the components to obtain the fermentation medium, inoculate the marine red yeast with high carotenoid production into it for staged inoculation and variable temperature fermentation, so as to maximize the proliferation of marine red yeast, and the fermentation liquid is homogenized by ultra-high pressure, vacuum centrifuged, and demulsified After separation, the emulsion is obtained, and the emulsion is extracted, separated and purified to obtain the finished carotenoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for producing carotenoids by fermenting yellow peach skin, comprising the steps of:

[0044] 1) Juice preparation: first put the yellow peach skin into a microwave dryer at a power of 4kW, a material layer thickness of 3cm, and dry at 110°C for 3min, then soak it in a sericin peptide solution with a mass percentage of 12% for 12min, and dry it at -20 After freezing at ℃ for 30 minutes, crush immediately. The thickness of the frozen material layer is 4 cm, and the particle size of the crushed product is 0.4 mm. Then, water twice the amount of the crushed product is added, and the pH value is adjusted to 4.5 with lactic acid. Time 400μs, pulse frequency 250Hz under the condition of high-voltage pulse electric field treatment for 25min; then microwave irradiation extraction at room temperature under the condition of power 200W for 18min, and at the same time conduct ultrasonic-assisted extraction under the condition of power 250W and frequency 35KHz; add 2.0% of th...

Embodiment 2

[0058] A method for producing carotenoids by fermenting defective yellow peach pulp, comprising the steps of:

[0059] 1) Juice preparation: first slice the defective yellow peach pulp, put it into a microwave dryer with a power of 3kW, a material layer thickness of 2cm, and dry at 105°C for 2 minutes, and then soak it in a sericin peptide solution with a mass percentage of 10% for 10 minutes , After freezing at -18°C for 20 minutes, crush immediately. The thickness of the frozen material layer is 3 cm, and the particle size of the crushed product is 0.3 mm. Then add water that is 1 times the amount of the crushed product, and adjust the pH value to 3.5 with lactic acid. / cm, pulse time 300μs, pulse frequency 200Hz under the conditions of high-voltage pulse electric field treatment for 20min; then microwave irradiation extraction at room temperature under the condition of power 150W for 15min, and ultrasonic-assisted extraction under the condition of power 200W and frequency 30...

Embodiment 3

[0073] A method for producing carotenoids by fermentation, comprising the steps of:

[0074] 1) Juice preparation: First, slice the residual yellow peach pulp into slices, then evenly mix it with the yellow peach skin at a mass ratio of 3:1, put it into a microwave dryer at a power of 5kW, a material layer thickness of 4cm, and dry at 115°C for 4min, then Soak in sericin peptide solution with a mass percentage of 14% for 15 minutes, freeze at -22°C for 40 minutes, and then crush immediately. The thickness of the frozen material layer is 5 cm, and the particle size of the crushed product is 0.5 mm. Lactic acid adjusts the pH value to 5.5, and conducts high-voltage pulse electric field treatment at room temperature under the conditions of electric field strength 35kV / cm, pulse time 500μs, and pulse frequency 300Hz for 30min; Under the conditions of power 300W and frequency 40KHz, ultrasonic-assisted extraction is carried out; mixed enzymes with 2.5% of the quality of the extract...

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Abstract

The invention discloses a method for producing carotenoid through fermentation. The method comprises the following steps: by taking yellow peach cannery trimmings as a main raw material, carrying out microwave fixation, frozen-breaking and biological enzymolysis to obtain fruit juice with relatively high carotenoid content, adjusting components to obtain a fermentation medium, inoculating oceanic red yeast producing the carotenoid with high yield into the fermentation medium, carrying out fractional inoculation and temperature shift fermentation so that maximization of proliferation of oceanic red yeast is realized, carrying out ultrahigh pressure homogenization, vacuum centrifugation and demulsification separation on fermentation broth to obtain emulsion, and extracting, separating and purifying the emulsion to obtain the carotenoid finished product, wherein the cell biomass of the fermentation broth is 38-52g / L, and the yield of the carotenoid is 47-61mg / L. The preparation method is simple, short in period, high in efficiency, energy-saving and environment-friendly, the whole course adopts a low temperature biologic processing technology, the production cost is reduced, the hidden environmental pollution danger is eliminated, and a solid foundation is laid for sustainable development of the technical field of deep processing of yellow peaches, so that the method disclosed by the invention has positive economic and social development significance.

Description

technical field [0001] The invention relates to the preparation of carotenoids, in particular to a method for producing carotenoids by fermentation. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or plant extraction. The chemical synthesis of carotenoids is not only te...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 李建树
Owner BEIJING KEHUITONG WISDOM SCI & TECH CO LTD
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