The invention relates to the technical field of mushroom strain fermentation, in particular to a fermentation method of mushroom liquid strains with the high activity and the high stability. The method comprises the steps that 1, preserved mushroom inclining strains are inoculated on a plating medium to be activated, advantageous hyphae are selected as a mother strain and inoculated in a shake flask containing a primary liquid culture medium, standing is performed under the temperature of 23+ / -2 DEG C for 36-48 h, the shake flask obtained after standing is placed into a constant temperature shaking table, oscillation culture is performed for 60 h, and the primary fermentation strain is obtained; 2, a fermentation tank is disinfected under the steam pressure of 0.16-0.18 Mpa and the temperature of 121 DEG C for 90-120 min, then, a secondary liquid culture medium is put into the fermentation tank, and sterilization is performed under the steam pressure of 0.15-0.16 Mpa and the temperature of 110 DEG C for 60 min; 3, after the temperature inside the fermentation tank is lowered to 28 DEG C or lower, the primary fermentation strain is inoculated into the fermentation tank, oxygen is additionally supplied, the tank pressure is 0.12-0.13 Mpa, and constant temperature culture is performed for 96 h; the fermentation method of mushroom liquid strains has the advantages that the technology is simple, operation is convenient, and the fermentation period is short, and has the good application prospect.