Method for preparing Rhodotorula benthica fermentation feed
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A technology for marine red yeast and fermented feed, which is applied in animal feed, animal feed, application and other directions, can solve problems that have not yet been seen, and achieve the effects of low fermentation cost, improved nutritional value, and improved digestion and absorption rate.
Inactive Publication Date: 2009-05-20
OCEAN UNIV OF CHINA
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Problems solved by technology
At present, there have been reports on the use of yeast, lactic acid bacteria, and Bacillus subtilis to ferment individually or in combination to remove anti-nutritional factors in soybean meal. See a report on the use of marine rhodotorula to ferment soybean meal to produce feed
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Embodiment 1
[0009] After crushing soybean meal, peanut meal and bran, mix the three evenly according to the weight ratio of 17:2:1 to make 1000g of soybean meal solid fermentation base material; in addition, 5g of yeast extract, KH 2 PO 4 0.2g, NaCl 1g, (NH 4 ) 2 SO 4 3.5g, 40g of sucrose and water are mixed to make 1000g nutritional supplement, and the pH value is adjusted to 6.0 with 1M HCl aqueous solution; the above-mentioned solid fermentation base material is mixed into the nutritional supplement, and the two are mixed evenly according to the weight ratio of 1:1.2, Make a solid fermentation medium, put it into a polyethylene plastic bag, tie the mouth of the bag tightly, cook at 100°C for 8 minutes, and cool.
[0010] When inoculating, first prepare Rhodotorula viscosus seed solution: first-level slant culture adopts potato medium, and after cultivating at 32°C for 24 hours, inoculate second-level marine yeast liquid medium, which consists of 2% glucose, 1% peptone, Yeast extra...
Embodiment 2
[0015] Crush soybean meal and peanut meal, and mix them evenly according to the weight ratio of 19:1 to make 1000g of soybean meal solid fermentation base material; in addition, 5g of yeast extract, KH 2 PO 4 0.1g, NaCl 1.5g, (NH 4 ) 2 SO 4 1.5g, 30g of sucrose and water are mixed to make 1000g nutritional supplement, and the pH value is adjusted to 6.0 with 1M HCl aqueous solution; the above-mentioned solid fermentation base material is mixed into the nutritional supplement, and the two are mixed evenly according to the weight ratio of 1:1.1, Prepare soybean meal solid fermentation medium, cook at 100°C for 8 minutes, and cool.
[0016] When inoculating, inoculate the seed solutions of Rhodotorula rubrum and Pediococcus lactis cultured to the logarithmic phase in the above-mentioned soybean meal solid fermentation medium at a ratio of 10% and 4%, respectively, and spread them on a tray with a thickness of 4cm after stirring evenly. Inside, cover tightly with a double-la...
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Abstract
The invention relates to a method for preparing ocean rhodotorula fermentation feed. The method comprises the preparation of solid fermentation base stock of soybean meal, the mixing of a nutrition replenishing agent and the preparation of a solid fermentation medium. The feed is characterized in that ocean rhodotorula and a lactobacillus strain are inoculated in the solid fermentation medium and are subjected to solid fermentation to obtain the ocean rhodotorula fermentation feed. The method has the advantages: 1. macromolecular protein in the soybean meal is decomposed into small molecular peptide which improves the digestive absorption rate of the protein and further balances the composition of amino acid through microbial transformation; 2. the fermentation feed is replenished with astaxanthin and other biological active substances introduced by the metabolism of rhodotorula glutinis, thereby improving the nutritive value of the feed; 3. lactobacillus in the fermentation feed has positive function on improving a microecological flora of intestinal tracts of bred animal; and 4. the method has low fermentation cost, low fermentation condition and equipment requirement, simple operation of process flow and small investment and is suitable for small-scale production of breeding households and feed processing factories.
Description
technical field [0001] The invention relates to a feed, in particular to a preparation method of a marine red yeast fermented feed. Background technique [0002] In recent years, with the development of my country's aquaculture industry, the demand for high-protein feed has increased year by year. The crude protein content of soybean meal is as high as 43% to 48%, and it has a relatively balanced amino acid composition, especially lysine that is easily lacking in other plant feeds, and has an excellent animal absorption rate, so it is favored by feed companies and farmers. It has become an indispensable protein feed with the largest proportion in the feed ratio. However, there are many anti-nutritional factors including trypsin inhibitors in soybean meal, which greatly affect the feeding value of soybean meal. Using biological fermentation to process soybean meal and then use it as bait for farmed animals can achieve the purpose of removing anti-nutritional factors without...
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