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Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof

A technology of YS-185 and marine red yeast, applied in the field of marine microorganisms, can solve problems such as no research reports, and achieve good stability and high anti-lipid peroxidation properties

Active Publication Date: 2010-05-12
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the strains mentioned in some literatures can produce higher astaxanthin content, such as Phodotorala rubra, Rhodotorula glutins, Flavobacterium spp C F-60 etc., but no detailed research report

Method used

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  • Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof
  • Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof
  • Astaxanthin-producing ocean rhodotorula YS-185 and method for producing astaxanthin thereof

Examples

Experimental program
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Embodiment 1

[0046] Marine Rhodotorula YS-185 culture preparation

[0047] Inoculate a lawn of Rhodotorula marine yeast YS-185 into a 250mL Erlenmeyer flask containing 30ml of seed medium, and culture it on a shaker at 20°C and 200r / min for 18h to obtain a seed solution. Inoculate in a 500mL Erlenmeyer flask with 90mL of seed liquid, and culture it on a shaker at 20°C and 200r / min for 18 hours to obtain a secondary seed liquid, then inoculate it into a fermenter with a 5% inoculation amount (10L tank contains 6L fermentation culture Base) was cultured at 20°C, and the dissolved oxygen was maintained at 45±2% by mass, the initial concentration of glucose was 3.0% by mass, and the pH was 4.8-5.0 by constant pH glucose-ammonia water feeding. The dry cell weight was 60.5mg / ml, and the astaxanthin yield was 66.5μg / ml.

[0048] Preparation and purification of crude pigment

[0049] Take the yeast cell powder prepared above, add dimethyl sulfoxide (20ml dimethyl sulfoxide per gram of bacterial ...

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Abstract

The invention relates to ocean rhodotorula YS-185 bred with high yield of astaxanthin, which is obtained from ocean, wherein the rhodotorula is preserved in CCTCC of Wuhan, China with the preservation number of CCTCC NO: M207077; the invention also relates to a method for producing astaxanthin by fermenting and cultivating the ocean rhodotorula YS-185. The astaxanthin produced by the ocean rhodotorula YS-185 is natural astaxanthin and can be widely applied to high-quality cosmetics, food and feed additives.

Description

technical field [0001] The invention relates to the field of marine microorganisms, in particular to a high-yield astaxanthin marine rhodotorula YS-185 and a method for producing astaxanthin. Background technique [0002] Microorganisms are important members of marine organisms, and the metabolic types of marine microorganisms are extremely diverse, and they can grow and reproduce at an astonishing rate under artificial control conditions, and the provenance of marine microorganisms is extremely rich, with many unknowns and great variability, so it is easy to select High-yielding strains are bred, so marine microorganisms are a new source of obtaining unknown strains and their high-yielding active substances in the world today, especially providing a broad way for sustainable development of the food industry and the pharmaceutical industry. However, due to the unique environment of the ocean, including high salinity , high pressure, low nutrition, low temperature, etc. have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P23/00C12R1/645
Inventor 孙谧刘均忠郝建华王跃军王伟
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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