Method of producing active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal

A technology for chrysanthemum meal and aquatic products, which is applied in the field of chrysanthemum meal fermentation to produce active peptide powder for aquatic products, livestock and poultry, which can solve the problems of excessive use of antibiotics and low protein utilization, and achieve the effects of improved growth performance, low price and convenient application

Inactive Publication Date: 2017-10-24
辽宁威兰生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in aquatic livestock and poultry farming, there are two major problems faced by produc

Method used

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  • Method of producing active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal
  • Method of producing active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal
  • Method of producing active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Preparation of arginine concentrated bacterial liquid dry powder:

[0080] Arginine bacteria producing strains are inoculated into medium containing glucose, peptone, ammonium sulfate and sodium glutamate and fermented for 64 hours to obtain arginine fermentation liquid. Put 16 tons of arginine fermentation broth into a 20-ton container and let it stand. Add sodium polyacrylate, a flocculant with a final concentration of 250ppm, to the arginine fermentation broth, adjust the pH value to 6.5 with sulfuric acid with a mass fraction of 40%, and react at a temperature of 50°C for a reaction time of 80min. Then, use a pressure of 3MPa to Put the reacted arginine fermentation hydraulic pressure into the filter press for 60 minutes to obtain arginine concentrated bacteria liquid wet powder with a water content of 35%, and dry it into 10% arginine concentrated bacteria Dry powder.

[0081] (2) Preparation of Bacillus subtilis seed liquid:

[0082] 1) Single colony isolation: a...

Embodiment 2

[0108] The chrysanthemum dregs obtained in Example 1 were fermented to produce active peptide powder for aquatic livestock and poultry in the application test in the culture of Penaeus vannamei. The design of the test scheme is shown in Table 1:

[0109] Table 1. Experimental design of active peptide powder for aquatic livestock and poultry production by fermentation of chrysanthemum meal

[0110] project

test 1

test 2

Test 3

control

Dosage

B+3％

B+4％

B+5％

bait (B)

[0111] The results of the application test of chrysanthemum meal fermentation to produce active peptide powder for aquatic livestock and poultry in Penaeus vannamei are shown in Table 2:

[0112] Table 2. Effects of active peptide powder for aquatic livestock and poultry produced by fermentation of chrysanthemum meal on weight gain, survival rate and feed return rate of Penaeus vannamei (30 days)

[0113]

[0114] *Water temperature 26°C; *Culture pool water are...

Embodiment 3

[0119] The chrysanthemum dregs obtained in Example 1 were fermented to produce active peptide powder for aquatic livestock and poultry in the application test of grouper breeding. The design of the test scheme is shown in Table 3:

[0120] Table 3. Experimental design of active peptide powder for aquatic livestock and poultry production by fermentation of chrysanthemum meal

[0121] project

test 1

test 2

Test 3

control

Dosage

B+3％

B+4％

B+5％

bait (B)

[0122] The application test results of chrysanthemum meal fermentation to produce active peptide powder for aquatic livestock and poultry in grouper breeding are shown in Table 4:

[0123] Table 4. Effects of active peptide powder for aquatic livestock and poultry produced by fermentation of chrysanthemum meal on weight gain, survival rate and feed return rate of grouper (30 days)

[0124]

[0125] *The control group used normal bait (B)

[0126]As can be seen from Table 4, u...

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Abstract

The invention relates to a method of producing an active peptide powder for aquatic products, livestock and poultry through fermentation of chrysanthemum meal. The method includes the steps of: 1) with the chrysanthemum meal, soybean meal, arginine concentrated bacterial slurry dried powder and corn steep liquor dry powder as raw materials and bacillus subtilis, arginine producing bacteria, rhodotorula benthica and lactobacillus plantarum as initial bacterial strains, respectively performing liquid seed cultivation and fermenting cultivation; 2) uniformly mixing a solid and a liquid according to ratio, placing the mixture on a fermentation bed and feeding sterilized air to perform aerobic solid fermentation cultivation; and 3) drying, crushing and sieving the product to obtain the active peptide powder for aquatic products, livestock and poultry. The active peptide powder has certain source of raw materials for fermentation, wherein the chrysanthemum meal, the arginine concentrated bacterial slurry dried powder and the corn syrup powder are low in cost and can produce a large amount of fermentation products. The active peptide powder is added to feeds for aquaculture and livestock and poultry breeding, thereby improving growth capability, improving product quality and also improving breeding environment. The active peptide powder is a green, environment-friendly and functional feed additive used for the aquatic products, livestock and poultry.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing active peptide powder for aquatic livestock and poultry by fermenting chrysanthemum dregs. Background technique [0002] Chrysanthemum meal, also known as chrysanthemum powder, is a by-product obtained by extracting lutein from marigold flowers grown in Northeast China, Inner Mongolia, Xinjiang and other places. It is green and fibrous, and its properties are similar to alfalfa powder. It is widely used in In livestock, poultry, fish and other feeds, it can replace bran and bran raw materials in various feed ratios, and is a very good green and health-care feed material. [0003] Main components of chrysanthemum meal: protein content 12%, ashes content 8%, moisture content 14%. Chrysanthemum meal is a pigment feed that can improve the skin color of animals and the color of eggshells, and is rich in various vitamins such as VC and VB. [000...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K10/30A23K20/142C12N1/20C12N1/16C12P1/02C12P1/04C12R1/125C12R1/645C12R1/25C12R1/15
CPCA23K10/12A23K10/30A23K10/37A23K20/142C12N1/16C12N1/20C12P1/02C12P1/04Y02P60/87
Inventor 刘志国李铁军张建华习凤安战晓燕薛德林
Owner 辽宁威兰生物技术有限责任公司
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