Method for fermenting phodotorula benthica culture
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A marine red yeast, a fermentation method technology, applied in the field of microbial culture fermentation, to achieve the effect of simple method process, increased weight gain speed, and improved reproductive performance
Active Publication Date: 2010-12-15
GUANGZHOU PROSYN BIOLOGICAL TECH CO LTD
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There is no report on the production process such as the wall breaking process of th
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Embodiment 1
[0025] 1. Strain: the yeast described in the present invention is marine rhodotorula. In the steps of strain isolation, screening and purification, the specific characteristics of fat-soluble brilliant red pigment can be shown in seawater or semi-seawater specific Rhodotorula medium, so as to determine the strain used.
[0026] 2. Culture medium: glucose 3.0%, peptone 1.0%, yeast extract 1.0%, ammonium sulfate 0.5, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, agar 2%, the balance is sea water or Half sea water. pH 6.8, cultured for 48 hours, it was confirmed that the strains could keep growing well in the above culture medium.
[0027] 3. Incline medium and culture conditions:
[0028] Glucose 3.0%, peptone 1.0%, yeast extract 1.0%, dipotassium hydrogen phosphate 0.1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%, agar 2.0%, the balance is seawater or semi-seawater, pH 6.8, extinguished at 115°C Bacteria fo...
Embodiment 3
[0040] The marine red yeast culture produced by the present invention is added 1 kg per ton of suckling pig full-price feed, feed is fed to suckling pigs 10 days after weaning, and the feed intake is measured in groups. As a result, using the product group of the present invention, the daily intake of suckling pigs Food intake increased by more than 30 grams compared with the control group.
[0041] Product assay result of the present invention:
[0042] Measured according to the national standard method, carotenoid content ≥ 20 μg / kg; yeast cell wall polysaccharide ≥ 100 mg / kg, crude protein content 10% to 50%; aflatoxin < 30 μg / kg.
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Abstract
The invention provides a method for fermenting a phodotorula benthica culture, which comprises the following steps of: (1) separating, screening and purifying phodotorula benthica strains; (2) performing liquid fermentation propagation and conversion on the purified phodotorula benthica strains; (3) performing solid aerobic fermentation on the phodotorula benthica strains subjected to the liquid fermentation propagation and conversion and performing solid anaerobic fermentated phodotorula benthica wall breakage; and (4) performing low-temperature drying. The method has the characteristics of simple production process, short period, low cost, stable product quality and the like. The product prepared by the method of the invention functions well in food appetite increasing, increasing of intestinal beneficial bacteria and antioxidation, and can be used for feeding livestock, poultry and aquatic fur-bearing animals as feedstuff or a feed additive.
Description
technical field [0001] The invention relates to a fermentation method of microbial culture, in particular to a production method of marine red yeast culture. Background technique [0002] Marine red yeast (Phodotorula benthica) is a kind of natural fungus with strong stress resistance existing in the ocean. The bacteria are round or oval, and the colonies are red and bright. It is rich in bioactive substances such as protein, amino acid, vitamin, unsaturated fatty acid, glycogen, carotenoid, astaxanthin and various unknown nutritional factors. Marine Rhodotorula has strong antioxidant capacity and is known as "super vitamin E". It has the functions of increasing food, benefiting the intestines and resisting diseases, and is mostly used in aquaculture. How to reduce the production cost of marine rhodotorula so that it can be more widely used in the breeding of livestock, poultry and aquatic animals has important practical significance. [0003] The Chinese patent applicati...
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