Method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application

A technology of immobilizing small balls and marine red yeast, which is applied in the field of environmental governance, can solve the problems of biological treatment of nitrogen and phosphorus nutrient concentration in aquaculture sewage, such as the difficulty of biological treatment, large amount of sewage, and small microalgae cells, so as to achieve less microbial loss , high algae cell density and strong poison resistance

Inactive Publication Date: 2020-11-24
GUANGZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the collection of microalgae after sewage treatment becomes a thorny issue due to the small size of microalgal cells
In addition, aquaculture sewage is characterized by low pollutant concentration, but the large amount of sewage and low concentration of nitrogen and phosphorus nutrients make the biological treatment of aquaculture sewage more difficult

Method used

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  • Method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application
  • Method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application
  • Method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Effects of different algae inoculation ratios on the removal of pollutants in sewage

[0066] (1) Preparation of immobilized chlorella and marine rhodotorula

[0067] Seawater Chlorella (Chlorella vulgaris) is immobilized after being cultivated in F / 2 medium to logarithmic growth phase; specifically, Chlorella vulgaris is inoculated in F / 2 medium and cultivated in a light incubator, The temperature is controlled at 25°C, the rotation speed is 150rpm, and the light intensity is 100μmol·m -2 ·s -1 , the light time is 24h, the light source is an incandescent lamp, cultivated to OD 680 = about 0.6, the dry weight of chlorella cells is about 2g / L.

[0068] The stock solution formula of F / 2 medium is as follows: NaNO 3 75mg / L, NaH 2 PO 4 ·H 2 O 5mg / L, Na 2 SiO 3 9H 2 O30mg / L, trace element solution 1mL / L, vitamin solution 0.5mL / L. The trace element solution formula is as follows: CuSO 4 ·5H 2 O (0.98g / 100mL) 1mL, ZnSO 4 ·7H 2 O (2.20g / 100mL) 1mL, Co...

Embodiment 2

[0086] Example 2: Study on the stability of the symbiosis system of immobilized chlorella and suspended marine rhodotorula in the removal of aquaculture sewage pollutants

[0087] After exploring in Example 1, it was determined that the inoculum ratio of immobilized Chlorella and suspended marine Rhodotorula was 5:1. Example 2 explored the stability and sustainability of the algae-bacteria symbiosis system under the semi-continuous experiment under this condition.

[0088] The mixture of immobilized seawater chlorella and suspended marine rhodotorula was inoculated into the aquaculture sewage at a ratio of 5:1. The concentration of immobilized chlorella in the sewage was controlled at 0.5g / L, and the semi-continuous experiment was repeated. Batch experiments, the specific steps are as follows:

[0089] The mixture of immobilized seawater chlorella and suspended marine rhodotorula was inoculated into the aquaculture sewage at a ratio of 5:1, and treated in a light incubator, t...

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Abstract

The invention provides a method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application, and belongs to the technical field of environmental governance. The immobilized chlorella and the rhodotorula benthica are commonly inoculated into the sewage, so as to treat the sewage. A symbiotic system of the immobilized chlorella and the rhodotorula benthica (inoculation ratio is 5 to 1) has relatively good removal effects to pollutants in cultivating sewage, wherein nitrate is completely removed within three cycles, the removal rate of ammonium nitrogen is 74.57%-86.19%, the removal rate of nitrite is 95% or above, the removal rate of nitrite is 90.68%-100%, the removal rate of COD is 92%-100%, and the data presents that an alga-bacteria symbiotic system has relatively good stability and sustainability.

Description

technical field [0001] The invention belongs to the technical field of environmental treatment, and in particular relates to a method and application for treating sewage by using immobilized chlorella and marine red yeast. Background technique [0002] Aquaculture has grown rapidly over the past 30 years, with total global fish production increasing from 13.2 million tonnes in 1990 to nearly 80 million tonnes in 2016. However, during the rapid development of mariculture, the discharge of aquaculture wastewater has also increased sharply. If there is no effective treatment method, it will lead to serious eutrophication of natural water bodies and bring damage to the ecological environment. In aquaculture waters, excess nitrogen-containing compounds can negatively affect aquaculture systems, especially NH, which is toxic to aquatic organisms 4 + and NO 2 - . Although conventional biological and physicochemical wastewater treatment systems can effectively remove pollutants...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C02F3/34C12N11/10C12N11/04C12N1/16C12R1/89C12R1/645C02F101/10C02F101/16C02F101/30
CPCC02F3/325C02F3/347C02F2101/105C02F2101/16C02F2101/30C12N1/16C12N11/04C12N11/10
Inventor 舒琥罗智展
Owner GUANGZHOU UNIVERSITY
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