Method for treating sewage by virtue of immobilized chlorella and rhodotorula benthica and application
A technology of immobilizing small balls and marine red yeast, which is applied in the field of environmental governance, can solve the problems of biological treatment of nitrogen and phosphorus nutrient concentration in aquaculture sewage, such as the difficulty of biological treatment, large amount of sewage, and small microalgae cells, so as to achieve less microbial loss , high algae cell density and strong poison resistance
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Embodiment 1
[0065] Example 1: Effects of different algae inoculation ratios on the removal of pollutants in sewage
[0066] (1) Preparation of immobilized chlorella and marine rhodotorula
[0067] Seawater Chlorella (Chlorella vulgaris) is immobilized after being cultivated in F / 2 medium to logarithmic growth phase; specifically, Chlorella vulgaris is inoculated in F / 2 medium and cultivated in a light incubator, The temperature is controlled at 25°C, the rotation speed is 150rpm, and the light intensity is 100μmol·m -2 ·s -1 , the light time is 24h, the light source is an incandescent lamp, cultivated to OD 680 = about 0.6, the dry weight of chlorella cells is about 2g / L.
[0068] The stock solution formula of F / 2 medium is as follows: NaNO 3 75mg / L, NaH 2 PO 4 ·H 2 O 5mg / L, Na 2 SiO 3 9H 2 O30mg / L, trace element solution 1mL / L, vitamin solution 0.5mL / L. The trace element solution formula is as follows: CuSO 4 ·5H 2 O (0.98g / 100mL) 1mL, ZnSO 4 ·7H 2 O (2.20g / 100mL) 1mL, Co...
Embodiment 2
[0086] Example 2: Study on the stability of the symbiosis system of immobilized chlorella and suspended marine rhodotorula in the removal of aquaculture sewage pollutants
[0087] After exploring in Example 1, it was determined that the inoculum ratio of immobilized Chlorella and suspended marine Rhodotorula was 5:1. Example 2 explored the stability and sustainability of the algae-bacteria symbiosis system under the semi-continuous experiment under this condition.
[0088] The mixture of immobilized seawater chlorella and suspended marine rhodotorula was inoculated into the aquaculture sewage at a ratio of 5:1. The concentration of immobilized chlorella in the sewage was controlled at 0.5g / L, and the semi-continuous experiment was repeated. Batch experiments, the specific steps are as follows:
[0089] The mixture of immobilized seawater chlorella and suspended marine rhodotorula was inoculated into the aquaculture sewage at a ratio of 5:1, and treated in a light incubator, t...
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