Composite microecological preparation and application thereof

A technology of compound microecology and preparation, applied in application, microorganism, microorganism-based method, etc., can solve the problems of unscientific compatibility of microecological preparations, single effect of microecological preparations, and narrow application range, so as to improve the anti-stress of animals. ability, improve the quality of farmed animal meat, and improve the effect of drug efficacy

Pending Publication Date: 2022-01-07
武安市益微益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The probiotics of a single strain have a single effect and a narrow application range in the actual application process, and the compatibility of the compound probiotics also has unscientific problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Compound probiotics

[0029] The compound microecological preparation of this embodiment is composed of Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans; wherein the ratio between Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans is 1:1:2, The total number of viable bacteria is ≥1010CFU / g, and the compound microecological preparation is added to the basic diet at a ratio of 0.1%.

[0030] (2) Experimental animals and basic diet

[0031]59,000 350-day-old Hailan brown commercial layer hens, the basic dietary formula is: 65.0% corn, 23.5% soybean meal, 1% soybean oil, 8% stone powder, 1.2% calcium hydrogen phosphate, 0.3% salt, 1% premixed Feed: Each kilogram of feed contains: VA 16mg, VD3 5mg, VE 20mg, VK3 3mg, VB1 4.6mg, VB2 10.5mg, VB6 8.6mg, VB12 2mg, protein powder 390mg, secondary powder 5473.55mg, calcium pantothenate 14.5mg, smoke Amide 40.2mg, folic acid 2.3mg, biotin 10mg, manganese sulfate 251.57mg, zinc sulfate 202.9mg, iro...

Embodiment 2

[0039] (1) Compound probiotics

[0040] The compound microecological preparation of this embodiment is composed of Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans; wherein the ratio between Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans is 1:2:2, The total number of viable bacteria is ≥1010CFU / g, and the compound microecological preparation is added to the basic diet at a ratio of 0.1%.

[0041] (2) Experimental animals and basic diet

[0042] 500 28-day-old weaned and healthy Du Long Sanyuan hybrid weaned piglets, with an average initial weight of 8kg, and a test period of 28 days. The basic diet formula was: corn 57%, soybean meal 17%, puffed soybean 10%, whey 5% flour, 3% wheat bran, 3% imported fish meal, 1% soybean oil, 4% premix: per kilogram compound feed: VA 300-400 KIU, VD3 50-250 KIU, VE500 IU, VK375 mg, VB135 mg , VB2100 mg, VB675 mg, VB120.5 mg, niacin 1000 mg, pantothenic acid 350 mg, folic acid 20 mg, biotin 1.1 mg, choline ch...

Embodiment 3

[0050] (1) Compound probiotics

[0051] The composite microecological preparation of this embodiment is composed of Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans; wherein the ratio between Phaffia rhodozyme, Clostridium butyricum, and Bacillus coagulans is 2:3:2, The total number of viable bacteria ≥ 1010CFU / g.

[0052] (2) Addition amount and fermentation conditions

[0053] The compound microecological preparation is mixed with compound feed or feed raw materials at a ratio of 0.3%, and then 700kg of water is evenly sprinkled on the feed, put into a plastic bag with a breathing valve, and fermented in a room above 20°C for 5 Fermentation is completed in 1 day. When fermentation starts, the breathing bag will bulge. After the fermentation is completed, the bulge will disappear.

[0054] (3) Fermentation effect

[0055] After the compound feed or feed material is fermented by the compound microecological preparation, the pH drops below 4.0, the content of...

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PUM

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Abstract

The invention discloses a composite microecological preparation and an application thereof. The composite microecological preparation comprises saccharomycetes, acid-producing bacteria and bacillus, wherein the viable count ratio of the saccharomycetes to the acid-producing bacteria to the bacillus is (1-3): (1-4): (1-2), the effective viable count of each gram of the composite microecological preparation is greater than or equal to 1010 CFU/g, the saccharomycetes are selected from at least one of saccharomyces cerevisiae, phaffia rhodozyma, rhodotorula mucilaginosa and candida utilis, the acid-producing bacteria are selected from at least one of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, clostridium butyricum and lactobacillus rhamnosus, and the bacillus is selected from at least one of bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens and bacillus megatherium. According to the composite microecological preparation and the application thereof, the intestinal health level of animals can be improved, the growth of the animals can be promoted, the anti-stress capability of the animals can be improved, the immune system of the animals can be activated, and diseases can be reduced.

Description

technical field [0001] The invention relates to the field of probiotics, in particular to a composite probiotic and its application. Background technique [0002] The probiotics of a single strain have a single effect and a narrow application range in the actual application process, and the compatibility of the compound probiotics also has unscientific problems. Contents of the invention [0003] The main purpose of the present invention is to provide a compound microecological preparation and its application, which can effectively solve the problems in the background technology. [0004] In order to achieve the above object, the technical scheme that the present invention takes is: [0005] A compound microecological preparation, including yeast, acid-producing bacteria, and bacillus, the ratio of viable bacteria of yeast, acid-producing bacteria, and bacillus is (1-3): (1-4): (1-2) . [0006] Preferably, the effective number of viable bacteria per gram of the compos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20A23K10/12A23K10/30C12R1/865C12R1/72C12R1/645C12R1/25C12R1/225C12R1/145C12R1/125C12R1/10C12R1/11C12R1/01
CPCC12N1/16C12N1/20A23K10/12A23K10/30A23V2400/175A23V2400/169Y02P60/87
Inventor 张军胡婷李晓明
Owner 武安市益微益生物科技有限公司
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