88results about How to "High content of small peptides" patented technology

The invention discloses a feed additive by mixedly fermenting shrimp heads and vegetable feed proteins and a processing method thereof. The method comprises the following steps of: performing squeezing and crushing, pulping with water, and filtration and separation on the shrimp heads to separate out shrimp shells so as to obtain a shrimp paste raw material; mixing the shrimp paste and the vegetable feed proteins according to a proportion, adding bacteria for solid fermentation, and drying and crushing to prepare fermented feed protein powder; and drying the separated shrimp shells to prepare raw materials of chitosan and shrimp shell powder. The method has the advantages of simple process, small equipment investment and low cost; and the feed additive has the advantages of full aroma of the fermented feed protein powder, good palatability, high utilization rate of proteins, rich beneficial functional nutrients such as astaxanthin, small peptide, probiotics and the like, wide application to aquatic and livestock and poultry feeds, improvement of survival rate, good animal growth effect, good feed attraction to aquatic animals, capacity of replacing fish meal in the livestock and poultry feeds, capacity of greatly replacing fish meal in high-grade aquatic feeds, remarkable reduction of feed cost, contribution to environmental protection, power saving, efficiency improvement, and remarkable social and economic benefits.

The invention discloses fish soluble feed protein and a processing method thereof. The processing method comprises the following steps of: mixing fish soluble and bean pulp, adding a strain into the mixture for solid-state fermentation, directly mixing the obtained product without drying and other raw materials in a formula and taking the mixed products as a feed for pigs, birds and cattle, or drying and crushing the obtained product to prepare the fish soluble feed protein used for protein raw materials in the feed industry and breeding industry after the fermentation is finished. The method has the advantages of simple process, low equipment investment and low cost; and the fish soluble feed protein is strong in fragrance and good in food attraction, improves protein content, is high in small peptide content and easy to digest and absorb, eliminates anti-nutritional factor in the bean pulp, is rich in various beneficial functional nutrient components such as probiotic and the like, plays active roles in improving weight gain and feed conversion rate, strengthening the immune function of organisms, improving production performance and the like, can greatly replace fish meal in livestock and poultry feeds and aquatic feeds, remarkably reduce feed cost, turn the fish soluble into the value from the waste, and is favorable for environmental protection as well as energy conservation and synergism.

The invention provides a complex bacterium for bean pulp fermentation. The complex bacterium is composed of bacillus subtilis, bacillus coagulans, aspergillus niger, aspergillus oryzae, lactobacillus plantarum and saccharomyces cerevisiae. The complex bacterium provided by the invention is obtained through repeated scientific experiment and reasonable combination based on physiological metabolism feature of the microorganism, the complex bacterium is vigorous in growth metabolism, is capable of secreting a plurality of enzymes, acids and other metabolites, and is capable of efficiently decomposing macromolecule protein, anti-nutritional factors and sensitization factors in the bean pulp, obviously increasing content of soluble protein, small peptide, organic acid, protease and vitamin and the number of probiotics and antibacterial peptide, improving the digestion utilization rate, inhibiting the growth of pathogenic bacteria and reducing the use of antibiotic; and meanwhile, the complex bacterium has acid fragrance of fermentation and is good in food attractant effect. The bacteria strains forming the complex bacterium are included in a feed additive variety catalogue (2013) issued by the department of agriculture, the complex bacterium is safe and reliable, and can be directly used for the industrial production for the bean pulp fermentation.

The invention relates to a preparation method of a soybean polypeptide protein feed. The method comprises the following steps: 1, screening bacterial strains; 2, preparing a seed liquid; 3, preparing a fermentation culture solution: preparing a mixed fermentation culture solution including Bacillus subtilis, microzyme and lactic acid bacteria; and 4, carrying out composite bacterium enzyme fermentation: fermenting a fermentation substrate (soybean meal) in the mixed fermentation culture solution, hydrolyzing the fermented substrate with an exogenous enzyme, drying above obtained hydrolysis product, and crushing the dried hydrolysis product to obtain the required soybean polypeptide protein feed. Compared with the prior art, the method has the advantages of simple process and easy realization of large-scale industrial production; and the prepared soybean polypeptide protein feed has the advantages of no bitter taste or peculiar smell, good mouthfeel, small dissolving viscosity, high content of small peptides, and good quality.

The invention discloses a method for producing a bean pulp feed additive. The method comprises the following steps: inoculating bacillus subtilis (Bacillus subtilis) CGMCC No.7690 into a seed medium and cultivating, so as to prepare a seed solution; inoculating the seed solution into a liquid fermentation medium, and gradually carrying out 10-fold amplified fermentation for 2-4 times, so as to obtain a liquid fermented bacterium solution; inoculating the liquid fermented bacterium solution into sterilized bean pulp; carrying out solid fermentation by virtue of a fermentation bed, controlling the temperature of the fermentation bed at 25-60 DEG C, the throughput at 0.1-10 L/min, and the fermentation time at 24-96 hours; reversing the materials for 1-5 times during fermentation, so as to obtain the bean pulp feed additive. Single-strain liquid-solid combined fermentation is carried out by adopting the bacillus subtilis; in the obtained bean pulp feed additive, the small peptide content and the crude protein content are significantly increased, and anti-nutritional factors are greatly degraded.

The invention relates to a method for producing small peptide amino acid microelement chelate by way of the acid hydrolysis of protein. Animal protein or vegetable protein is adopted as raw material, and is hydrolyzed by hydrochloric acid. Hydrolysate is subjected to rough filtration and microfiltration, filtrate is deacidified by a nanofiltration membrane separation system, and the deacidified liquor is purified by way of ultrafiltration separation. Obtained small peptide and amino acid, the molecular weight of which is less than 1000 Dalton, are chelated with microelements, so that the small peptide amino acid microelement chelate is produced. The method is suitable for industrial production, the production cycle is short, the product yield is high, the three wastes cannot be produced in the process of production, and the product has good chemical stability, is rich in nutrition, can be easily digested and absorbed, can resist interference, is nonirritant, has the effect of improving the immunity of body, and is an ideal food nutrition enhancer and feed additive. The invention has a positive and profound significance on promoting the development of feed industry and breeding industry, enhancing the safety of poultry, livestock and aquatic products and protecting the health of human being.

The invention relates to the field of livestock feed, and discloses a fermented feed containing rapeseed meal, and a preparation method of the fermented feed. The preparation method comprises the steps of carrying out solid state fermentation on fermentation raw materials including the rapeseed meal, compound microbial strain, sodium hydroxide and water, and then drying to obtain the fermented feed containing the rapeseed meal. According to the preparation method, lactobacillus plantarum, bacillus subtilis, bacillus licheniformis and saccharomyces cerevisiae are taken as microbial strains for the solid state fermentation of the rapeseed meal, and the aim of remarkably improving the contents of protein and small peptide of the rapeseed meal can be realized in the environment of solid state fermentation in virtue of metabolic activity and interaction of all microorganisms, so that the application of the rapeseed meal in the feed can be further expanded.

The invention relates to a foraging feed of a special compound feed for breeding stichopus japonicus and a preparation method of the foraging feed. The technical problems of poor disease control, high cost and the like in conventional breeding of the stichopus japonicus are solved. The foraging feed is prepared through the following steps of adding mixed microbe fluid to the following raw materials in parts by weight of 7-13 parts of soybean meal, 6-9 parts of sweet potato residues, 5-8 parts of corn steep liquor, 5-10 parts of sea mud, 3-5 parts of bran, 8-10 parts of soybean protein concentrate, 5-8 parts of yeast protein, 6-10 parts of wheat flour, 3-6 parts of shrimp powder, 3-5 parts of spirulina powder, 5-10 parts of sea spinach powder, 0.5-1.6 parts of sodium alginate, 0.5-1 part of bentonite, 4-8 parts of betaine, 0.5-4 parts of immune polysaccharide, 0.3-0.5 part of taurine, 0.5-2 parts of honeysuckle flowers, 1-3 parts of radix astragali, 0.5-1 part of gypsum, 0.8-2 parts of haws, 1-3 parts of licorice roots, 1-3 parts of activated carbon, 0.5-1.0 part of calcium propionate, 3-5 parts of complex vitamins, 0.5 part of complex enzymes and the like, and performing fermentation. The foraging feed is prepared through the following steps of mixing the raw materials, performing crushing, performing screening, performing fermentation, performing drying and the like. The foraging feed of the special compound feed is widely applied to breeding of the stichopus japonicus.

The invention provides a preparation method of a liquid feed. The preparation method comprises the following steps of performing crushing; performing enzymolysis: performing enzymolysis treatment on raw materials needing to be fermented, through high-temperature amylase, so as to obtain feed liquid after enzymolysis; performing fermentation: performing anaerobic fermentation treatment on the feedliquid through lactic acid bacteria, neutral protease and acid protease so as to obtain fermented feed liquid; performing mixing: adding surplus raw materials to the fermented feed liquid so as to obtain mixed feed liquid; and performing acid adjustment: detecting the pH value of the mixed feed liquid, and after the pH value conforms to requirements, obtaining the liquid feed. The liquid feed prepared by the preparation method disclosed by the invention is rich in lactic acid, small peptide, complex enzymes, immunoglobulin and the like, has the characteristics of being balanced in nutrition, good in palatability, high in digestibility and the like, and can effectively alleviate weaning stress syndromes of piglets, so that the growth properties of weaned piglets can be improved, and the mortality rate of the weaned piglets can be reduced. The invention also discloses an application of the liquid feed. The liquid feed is mainly applied to the weaned piglets, so that the growth propertiesof the piglets are better, and the survival rate of the piglets is higher.

The invention discloses bacillus amyloliquefaciens which is named DHN04 and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No:60376. The bacillus amyloliquefaciens can efficiently secrete neutral protease, has obvious effect on degrading antigen protein of soybean meal and increasing the content of small peptide of fermented soybean meal, and is apotential soybean meal fermentation strain and a microecological preparation. The invention also provides a soybean meal fermentation method. By means of fermented seed liquid of the bacillus amyloliquefaciens, soybean globulin and beta-associated soybean globulin can be subjected to enzymatic hydrolysis effectively, and thus the proportion of the small peptide is greatly increased.

The invention provides a method for producing fish meal by using enzymatic hydrolysis and fermentation, comprising the steps of S1, adding complex enzyme to deoiled fish for enzymatic hydrolysis; S2, adding a compound bacterium to the material enzymatically hydrolyzed in S1 for semi-solid mixed fermentation, wherein the complex enzyme in S1 is of papain, neutral protease and bromelain, and the compound bacterium in S2 is prepared by culturing Lactobacillus acidophilus, Bacillus subtilis and Saccharomyces cerevisiae respectively into seed solutions, centrifuging the seed solutions sequentially, and mixing the bacteria acquired by centrifuging, the addition of the complex enzyme is 0.5-3%, pH in the enzymatic hydrolysis is adjusted to 5-6, enzymatic hydrolysis temperature is 45-55 DEG C, and enzymatic time is 40-180 min. The method for converting fish meal is simple, the fish meal has high content of small and medium peptides, and protein availability is high. In addition, by fermenting, mass beneficial microbes and complex metabolites can be imparted to the fish meal, and the quality of the fish meal can be effectively improved.

The invention relates to a peptide protein feed which includes: feed cake meal, fermented bacterial liquid, mannase, xylanase, cellulase, dextranase, molasses and water. A preparation method includes the steps of: (S1) preparing lactic acid bacteria liquid and yeast liquid; (S2) performing stacking fermentation to the raw materials; and (S3) drying and crushing the fermented raw materials and packaging the raw materials to obtain the peptide protein feed. The peptide protein feed is reduced in production cost, is effectively increased in contents of small peptides and organic acids and degradation quantity of anti-nutritional factors, is improved in palatability and is increased in intake, can reduce stinky smell of feeding animals and reduce defecation, improves digestion and absorption, increases production benefit and is green and environment-friendly.

The invention belongs to the field of biological feed, and specifically relates to a processing method and application of fermented feed for aquatic products based on hermetia illucens. The method comprises the following steps: activating a strain to obtain a bacillus fermentation seed solution, a saccharomycetes fermentation seed solution and a lactic acid bacteria fermentation seed solution; mincing and homogenizing fresh hermetia illucens larvae, and then mixing with bran, rice bran and wheat middling, adding acid protease, conducting mixing to obtain a fermentation culture medium, inoculating the bacillus fermentation seed solution, the saccharomycetes fermentation seed solution and the lactic acid bacteria fermentation seed solution, conducting anaerobic fermentation under the closed condition, and then obtaining a finished product. According to the present invention, fresh hermetia illucens are adopted for bacterium-enzyme synergistic fermentation, fermentation is thorough, and the finished product is high in small peptide content and rich in organic acid and has antibacterial activity; and meanwhile, the storage time is long, use is convenient, and good economic benefits are achieved.

The invention discloses a composite microecological preparation and an application thereof. The composite microecological preparation comprises saccharomycetes, acid-producing bacteria and bacillus, wherein the viable count ratio of the saccharomycetes to the acid-producing bacteria to the bacillus is (1-3): (1-4): (1-2), the effective viable count of each gram of the composite microecological preparation is greater than or equal to 1010 CFU/g, the saccharomycetes are selected from at least one of saccharomyces cerevisiae, phaffia rhodozyma, rhodotorula mucilaginosa and candida utilis, the acid-producing bacteria are selected from at least one of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, clostridium butyricum and lactobacillus rhamnosus, and the bacillus is selected from at least one of bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus amyloliquefaciens and bacillus megatherium. According to the composite microecological preparation and the application thereof, the intestinal health level of animals can be improved, the growth of the animals can be promoted, the anti-stress capability of the animals can be improved, the immune system of the animals can be activated, and diseases can be reduced.

The invention discloses fermented melon seeds rich in small peptides and a processing method thereof. Fermentation is carried out by adding clear water, complex probiotics and glucose to hulled dry melon seeds, and then the melon seeds are dried to prepare the fermented melon seeds. The complex probiotics are composed of bacillus subtilis, brewers' yeast, aspergillus niger and bifidobacteria. The processing method of the fermented melon seeds comprises the following steps: washing fresh melon seeds, and drying until water content is below 10% so as to prepare hulled dry melon seeds; adding the complex probiotics and glucose into clear water and uniformly stirring; adding the hulled dry melon seeds into a product in the step B and uniformly stirring; sealing and fermenting at 28-32 DEG C for 2-7 days; taking out the melon seeds, and drying at 50-75 DEG C until water content is below 10%; and carrying out sealed packaging. According to the invention, content of small peptides in melon seeds can be remarkably raised, antinutritional factors are eliminated, fat and energy are reduced, toxins are decreased, and lots of probiotics are generated. The invention provides a brand-new approach to development of new melon seed products.

The invention discloses a method for producing feed through enzyme-linked micro-ecologic solid state fermentation of vinasse. The method comprises the following steps: 1, carrying out primary fermentation: drying fresh vinasse until the water content is 10-25%, adding wheat bran and a meal component, mixing the dried vinasse, the wheat bran and the meal component to form a fermentation raw material, inoculating the fermentation raw material with a working fluid, adding a probiotic compound enzyme, placing the obtained mixture in a single-hole breathing bag, and carrying out enzymatic hydrolysis fermentation to obtain the biological feed; and 2, carrying out semi-conservative continuous fermentation: taking a part of the biological feed to substitute the working fluid in step 1, and repeating the step 1 to continuously obtain the biological feed. The proper animal probiotic strain and the compound enzyme are selected, and the semi-conservative continuous fermentation is carried out, so nutrients and precursors are supplemented, and metabolized harmful substances are diluted, thereby continuous synthesis of the above product and further degradation of anti-nutrition factors are facilitated, the nutrition values of the vinasse are greatly improved, the added values of the vinasse are increased, the storage and long-distance transportation of the feed are convenient, and the method has good social and economic benefits and environmental protection meaning.

The invention relates to a selenium-enriched August melon and fruit powder product and a production process thereof. The selenium-enriched August melon and fruit powder product is obtained through preparation of enzymatic hydrolysis liquid of August melon, fermentation of blueberry pomace, extraction of blueberry pigment, mixing and spray drying . The invention reduces the viscosity of August melon and fruit powder, increases the content of organic selenium and amino acid in the fruit powder, and improves the taste and color of the fruit powder. Through the action of microorganisms, it can effectively remove green and astringent substances, reduce the damage of microorganisms to pigments, and ensure the content of blueberry anthocyanins to the greatest extent. Through the joint action of enzymatic degradation and yeast fermentation, the content of small peptides in the fruit powder is greatly increased, and the characteristics of yeast autolysis are fully utilized to make the fruit powder contain a large amount of antioxidant substances.

The invention discloses a fish cream fermented soybean pulp and a production method and an application thereof. The method provided by the invention comprises the following steps of (1) preparing soybean pulp, concentrated fish soluble which is subjected to field preservation treatment and contains 50-75wt% of moisture, and pineapple juice which is subjected to field preservation treatment; (2) mixing the raw materials according to the ratio that 200-500kg of concentrated fish cream is used for each ton of soybean pulp, and 100-200kg of pineapple juice is used for each ton of soybean pulp and adding yeast seeds in the mixture; and (3) performing solid fermentation. The product prepared by the method provided by the invention is particularly suitable for the special nutrition demands of young farmed animals, the product is good in palatability, high in digestibility, and the product further has the natural lactation promotion function, thus, the product can improve the milk quality of suckling animals.