Method for preparing peptide used for active feed by bacteria and enzyme synergistic fermentation bean pulp

A technology of synergistic fermentation and feed peptide, applied in the application, animal feed, animal feed, etc., can solve the problems of the digestibility of soybean meal material and the decrease of animal absorption rate.

Active Publication Date: 2013-03-06
浙江诚元生物技术有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The existence of these anti-nutritional factors either inhibits the production of some digestive enzymes in animals, or complexes with nutrients to form indigestible components, which reduces the digestibility of soybean meal materials and the absorption rate of animals.

Method used

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  • Method for preparing peptide used for active feed by bacteria and enzyme synergistic fermentation bean pulp

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Experimental program
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Embodiment 1

[0031] Embodiment 1: A method for preparing active feed peptides by fermenting soybean meal with enzymes, comprising the following steps:

[0032] A) A total of 10kg of compound enzymes used for the preparation of collaborative fermentation. The compound enzyme includes neutral protease, acid protease, alkaline protease, non-starch polysaccharide enzyme, pectinase and xylanase. Among them, neutral protease, acid protease and alkaline protease are secreted by Bacillus or Aspergillus niger. Meanwhile, the parts by weight of each component are: 15 parts of neutral protease, 10 parts of acid protease, 8 parts of alkaline protease, 18 parts of non-starch polysaccharide enzyme, 6 parts of pectinase and 3 parts of xylanase. During the preparation, the compound enzyme was activated by warm water at 40oC for 1 hour. During the activation, stir well to make all kinds of enzymes mix evenly and fully dissolve.

[0033] B) Prepare 10kg of composite strains for collaborative fermentation...

Embodiment 2

[0042] Example 2: The method of enzyme synergistic fermentation of soybean meal to prepare active feed peptides, the only difference from Example 1 is:

[0043] In step A), in parts by weight, the parts by weight of the components in the compound enzyme used for the synergistic fermentation are: 20 parts of neutral protease, 15 parts of acid protease, 12 parts of alkaline protease, 24 parts of non-starch polysaccharide enzyme, fruit 11 parts of gluease, 8 parts of xylanase. During the preparation, the compound enzyme was activated by warm water at 45oC for 1.5h. During the activation, stir well to make all kinds of enzymes mix evenly and fully dissolve.

[0044]In step B), in parts by weight, the parts by weight of the components of the composite bacteria used for the synergistic fermentation are: 20 parts of Bacillus lentus, 30 parts of Candida utilis, and 30 parts of Lactobacillus fermentum. During the preparation, each bacterial strain of the composite bacterial strain is...

Embodiment 3

[0053] Example 3: The method of enzyme synergistic fermentation of soybean meal to prepare active feed peptides, the only difference from Example 1 is:

[0054] In step A), in parts by weight, the parts by weight of the components in the compound enzyme used for the synergistic fermentation are: 23 parts of neutral protease, 18 parts of acid protease, 16 parts of alkaline protease, 26 parts of non-starch polysaccharide enzyme, fruit 13 parts of gluease, 2 parts of xylanase. During preparation, the complex enzyme was activated by warm water at a temperature of 50°C for 2 hours. During the activation, stir well to make all kinds of enzymes mix evenly and fully dissolve.

[0055] In step B), in parts by weight, the parts by weight of the components of the composite bacteria used for the synergistic fermentation are: 27 parts of Bacillus lentus, 36 parts of Candida utilis, and 35 parts of Lactobacillus fermentum. During the preparation, each bacterial strain of the composite bac...

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Abstract

The invention discloses a method for preparing peptide used for the active feed by the bacteria and enzyme synergistic fermentation bean pulp. The method comprises the following steps of: preparing complex enzyme used for synergistic fermentation; preparing complex strain used for the synergistic fermentation; mixing and adding the complex enzyme and the complex strain into the bean pulp; blending the bean pulp with warm water; carrying out grading temperature-control fermentation on the bean pulp containing water; and post-processing materials formed after fermentation to obtain a finished product. Compared with the prior art, the method has the advantages that trypsin inhibitor is almost removed, big-molecule protein in the bean pulp is effectively degraded, and the actions of the microorganism and the enzyme are furthest cooperated.

Description

technical field [0001] The invention relates to the technical field of microbial enzymatic hydrolysis and fermentation, in particular to a method for preparing active feed peptides by synergistically fermenting soybean meal with bacteria and enzymes. Background technique [0002] As a large agricultural country, the aquaculture industry occupies an important position in my country's agriculture, and feed is an important foundation for the aquaculture industry. Usually, the content and quality of protein in feed are important criteria for evaluating its quality, and it is also directly related to the health and production level of animals. Feed protein raw materials can be divided into two categories: animal protein raw materials and vegetable protein raw materials. Among them, animal protein raw materials include fish meal, meat and bone meal, and plasma protein powder. Because the amino acid composition of animal protein is similar to the amino acids needed for the growth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14
Inventor 蔡祥敏方乐杨玉蓉蔡发展唐坚
Owner 浙江诚元生物技术有限公司
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