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Method for producing feed through enzyme-linked micro-ecologic solid state fermentation of vinasse

A solid-state fermentation and micro-ecological technology, which is applied in the forming or processing of animal feed, animal feed, animal feed, etc., can solve the problems of less active microorganisms, difficult to degrade cellulose, and low feed utilization rate, and achieve simple operation and increased efficiency. The effect of large breeding volume and reduced production cost

Inactive Publication Date: 2017-04-19
WUHAN SUNHY BIOLOGICAL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a new method of enzyme-linked micro-ecological solid-state fermentation of distiller's grains to improve the utilization of distiller's grains in order to solve the problems of difficult degradation of cellulose, less active microorganisms, poor palatability, etc. during the solid-state fermentation of distiller's grains. rate, increase its added value, reduce production costs, and obtain highly nutritious biological feed

Method used

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Experimental program
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Effect test

Embodiment 1

[0033] 1. Initial fermentation

[0034] 1. Dehydration pretreatment of fresh distiller's grains

[0035] The fresh grains with a moisture content of about 20% to 90% are dried until the moisture content is about 10% to 25%, which is used for nutrient ratio and fermentation cultivation.

[0036] 2. Pre-activation of distiller's grain compost before fermentation

[0037] Since vinasse is a by-product of brewing, the content of reducing sugar is reduced, that is, there are less carbon sources that microorganisms can easily use. Therefore, about 2%-15% of wheat bran and meal are added to the pretreated and dehydrated dregs as a supplementary carbon source, and mixed to make fermentation raw materials. The protein content of the final fermentation raw materials is 20%-35%. Moisture 5% to 60%.

[0038] 3. Preparation of working solution

[0039] Mix Bacillus subtilis, yeast and lactic acid bacteria with a certain proportion of molasses to form a working solution. The proportion...

Embodiment 2

[0057] Take 1000g of fresh distiller's grains (with a water content of about 70%), 10g of meal, 10g of wheat bran, 6g of yeast and enzyme mixture, and ferment the mixed microorganisms at 28-37°C for 24 hours, and mix them with the initial fermentation raw materials, and the water content is about 40%. ~52%, placed in a single-hole breathing bag at 40-45°C for 36-48 hours of enzymatic hydrolysis and fermentation, which is the finished product of the initial fermentation; take part of the finished product of the initial fermentation, mix it into the pre-mixed fermentation raw materials in equal proportions, and add 0.1-0.5% probiotics Mix the compound enzyme and place it in a single-hole breathing bag at 40-45°C to continue enzymatic hydrolysis and fermentation for 12-24 hours. After the fermentation is completed, directly pack the wet material into the bag.

[0058] Detect the number of active probiotics in the final fermented product, such as yeast reaching 8.5×10 9 CFU / g, Bac...

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Abstract

The invention discloses a method for producing feed through enzyme-linked micro-ecologic solid state fermentation of vinasse. The method comprises the following steps: 1, carrying out primary fermentation: drying fresh vinasse until the water content is 10-25%, adding wheat bran and a meal component, mixing the dried vinasse, the wheat bran and the meal component to form a fermentation raw material, inoculating the fermentation raw material with a working fluid, adding a probiotic compound enzyme, placing the obtained mixture in a single-hole breathing bag, and carrying out enzymatic hydrolysis fermentation to obtain the biological feed; and 2, carrying out semi-conservative continuous fermentation: taking a part of the biological feed to substitute the working fluid in step 1, and repeating the step 1 to continuously obtain the biological feed. The proper animal probiotic strain and the compound enzyme are selected, and the semi-conservative continuous fermentation is carried out, so nutrients and precursors are supplemented, and metabolized harmful substances are diluted, thereby continuous synthesis of the above product and further degradation of anti-nutrition factors are facilitated, the nutrition values of the vinasse are greatly improved, the added values of the vinasse are increased, the storage and long-distance transportation of the feed are convenient, and the method has good social and economic benefits and environmental protection meaning.

Description

technical field [0001] The invention relates to the technical field of recovery and utilization of distiller's grains, in particular to an enzyme-linked micro-ecological solid-state fermentation method for distiller's grains to produce feed. Background technique [0002] As a big wine-making country, our country is rich in distiller's grain resources. According to statistics, the annual output of distiller's grains is about 7 million tons (in terms of dry matter). At present, the utilization rate of distiller's grains is not high, and there are two restrictive factors that limit the full utilization of distiller's grains resources: one is the high acidity of fresh distiller's grains (pH is about 4), high water content (60% to 65%), and it is easy to spoil and deteriorate, which is not conducive to Preservation and long-distance transportation can only be used locally; the second is that a large amount of rice husk has been added to the fermented grains during the liquor mak...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/38A23K10/37A23K10/14A23K10/10A23K10/18A23K10/33A23K40/00
CPCY02P60/87
Inventor 毛玲陶敏周樱詹志春
Owner WUHAN SUNHY BIOLOGICAL
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