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Preparation method of soybean polypeptide protein feed

A protein feed and soybean polypeptide technology, which is applied in the field of preparation of polypeptide protein raw materials, can solve the problems of high protein content of macromolecules, poor taste of fermented soybean meal, and difficulty in animal absorption, and achieves simple production process, low solubility viscosity, and improved productivity. Effect

Inactive Publication Date: 2016-12-07
上海邦成生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent solves the technical problems of incomplete fermentation and low nutritional value of soybean meal to a certain extent, the fermented soybean meal prepared by it has a poor taste and high protein content, which is not easy for animals to absorb

Method used

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Effect test

Embodiment 1

[0031] A method for preparing a soybean polypeptide protein feed, the method comprising the following process steps: take 300kg of soybean meal, ultrafinely pulverize and mix evenly, add 70kg of water as a mixing material, and add 0.9kg of sucrose, then cook and sterilize, after sterilization When the temperature of the fermentation substrate is lowered to 30°C, add the mixed fermentation culture liquid and stir evenly. During the mixing process, adjust the water content of the material to 40%, and first introduce ambient air into the fermentation tank to establish the required conditions, aerobic Ferment for 6 hours, followed by anaerobic fermentation for 48 hours, and the fermentation temperature is controlled at 32°C. After the fermentation, the temperature of the fermentation substrate was raised to 50°C, and 0.15 kg of exogenous enzyme was added to carry out the hydrolysis reaction; after the hydrolysis, the material was heated at 80°C for 30 minutes, dried at low temperat...

Embodiment 2

[0034] A preparation method of soybean polypeptide protein feed, the method comprising the following process steps: take 500kg of soybean meal, superfinely pulverize and mix evenly, add 100kg of water as a mixing material, and add 2.5kg of sucrose, then cook and sterilize, after sterilization The temperature of the fermentation substrate dropped to 40°C, and the mixed bacterial solution was added, and stirred evenly. During the mixing process, the water content of the material was adjusted to 50%, and the ambient air was first introduced into the fermenter to establish the required conditions, and the aerobic fermentation was carried out for 8 hours. , followed by anaerobic fermentation for 40 hours, and the fermentation temperature was controlled at 32°C. After the fermentation, the temperature of the fermentation substrate was raised to 50°C, and 0.5 kg of exogenous enzyme was added to carry out the hydrolysis reaction; after the hydrolysis, the material was heated at 80°C fo...

Embodiment 3

[0037] A preparation method of soybean polypeptide protein feed, the method comprising the following process steps: take 200kg of soybean meal, superfinely pulverize and mix evenly, add 50kg of water as a mixing material, and add 0.2kg of sucrose, then cook and sterilize, after sterilization When the temperature of the fermentation substrate is lowered to 30°C, add the mixed bacterial liquid and stir evenly. During the mixing process, adjust the water content of the material to 60%, and first pass ambient air into the fermenter to establish the required conditions. Aerobic fermentation for 10 hours , followed by anaerobic fermentation for 48 hours, and the fermentation temperature was controlled at 32°C. After the fermentation, the temperature of the fermentation substrate was raised to 50°C, and 0.16 kg of exogenous enzyme was added to carry out the hydrolysis reaction; after the hydrolysis, the material was heated at 80°C for 30 minutes, dried at low temperature and crushed t...

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Abstract

The invention relates to a preparation method of a soybean polypeptide protein feed. The method comprises the following steps: 1, screening bacterial strains; 2, preparing a seed liquid; 3, preparing a fermentation culture solution: preparing a mixed fermentation culture solution including Bacillus subtilis, microzyme and lactic acid bacteria; and 4, carrying out composite bacterium enzyme fermentation: fermenting a fermentation substrate (soybean meal) in the mixed fermentation culture solution, hydrolyzing the fermented substrate with an exogenous enzyme, drying above obtained hydrolysis product, and crushing the dried hydrolysis product to obtain the required soybean polypeptide protein feed. Compared with the prior art, the method has the advantages of simple process and easy realization of large-scale industrial production; and the prepared soybean polypeptide protein feed has the advantages of no bitter taste or peculiar smell, good mouthfeel, small dissolving viscosity, high content of small peptides, and good quality.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide protein raw material, in particular to a preparation method of a soybean polypeptide protein feed. Background technique [0002] Soybean meal is the most commonly used vegetable protein raw material in animal diets, but because of the presence of various anti-nutritional factors in soybean meal, such as soybean antigenic protein and trypsin inhibitor, the application of soybean meal in young animal diets has been greatly reduced. Therefore, it is necessary to process the soybean meal to remove the anti-nutritional factors in the soybean meal and improve the nutritional quality of the soybean meal. At present, there are various processing techniques for soybean meal. In China, solid-state fermentation is the main method. Bacteria, yeast, and lactic acid bacteria are mostly used as one or a combination of several types of bacteria. The fermentation substrate can be directly added to the ferme...

Claims

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Application Information

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IPC IPC(8): A23K1/00C12P21/06
Inventor 郑锐郑云峰陆克文
Owner 上海邦成生物工程有限公司
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