Processing method and application of fermented feed for aquatic products based on hermetia illucens
A technology of fermented feed and processing method, which is applied in application, animal feed, animal feed, etc., can solve the problems of black soldier fly storage and transportation difficulties, lower digestion and absorption rate of the body, and lower growth performance of the body, so as to achieve good feed effect, Improve digestibility and enhance the body's immunity
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Embodiment 1
[0037] (1) Activation culture of bacillus, yeast and lactic acid bacteria:
[0038] A, first configure the culture medium of bacillus, saccharomyces and lactic acid bacteria;
[0039] Bacillus liquid and solid medium: tryptone 10g, sodium chloride 10g, yeast powder 5g, distilled water 1L, pH natural, sterilized at 121°C for 20min, the composition of the Bacillus solid medium is the same as that of the liquid medium; the difference is that 15g of agar is added .
[0040] Yeast liquid and solid medium: 200g potatoes boiled and filtered through 8 layers of gauze, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, distilled water to 1L, pH natural, sterilized at 121°C for 20min; The composition of the solid medium is the same as that of the liquid medium, the difference is that 15g of agar is added;
[0041] Lactic acid bacteria liquid and solid medium: beef extract 10g, tryptone 10g, glucose 20g, yeast powder 5g, dipotassium hydrogen phosphate 2g, diammoniu...
Embodiment 2
[0051] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;
[0052] (2) Configure the anaerobic fermentation medium, and the weight percentages of each component are respectively: black soldier fly homogenate 55% (water content 70%); bran 11.2%; rice bran 20%; secondary powder 13%; acidity Protease 0.8%, mixed uniformly to obtain anaerobic fermentation medium.
[0053] (3) Inoculate the fermented seed liquid of Bacillus natto, the fermented seed liquid of Saccharomyces cerevisiae, the fermented seed liquid of Lactobacillus plantarum, the seed liquid of Lactobacillus fermentum and the seed liquid of Lactobacillus rhamnosus after the activation culture of step (1) into the fermentation culture Base, the inoculum of Bacillus natto and Saccharomyces cerevisiae is 1.2% of the fermentation medium weight, the total number of live bacteria of Bacillus natto and Saccharomyces cerevisiae is 2.3 × 10 9 cfu / mL, the ino...
Embodiment 3
[0058] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;
[0059] (2) Configure the anaerobic fermentation medium, and the weight percentages of each component are respectively: black soldier fly homogenate 48% (water content 70%); bran 20%; rice bran 14.7%; secondary powder 16%; acidity Protease 1.3%, mixed uniformly to obtain anaerobic fermentation medium.
[0060] (3) Inoculate the fermented seed liquid of Bacillus natto, the fermented seed liquid of Saccharomyces cerevisiae, the fermented seed liquid of Lactobacillus plantarum, the seed liquid of Bacillus coagulans and the seed liquid of Lactobacillus paracasei after the activation culture of step (1) into the fermentation medium , the inoculum of Bacillus natto and Saccharomyces cerevisiae is 1% of the weight of the fermentation medium, and the total number of live bacteria of Bacillus natto and Saccharomyces cerevisiae is 2.6 × 10 9 cfu / mL, the inoc...
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