Processing method and application of fermented feed for aquatic products based on hermetia illucens

A technology of fermented feed and processing method, which is applied in application, animal feed, animal feed, etc., can solve the problems of black soldier fly storage and transportation difficulties, lower digestion and absorption rate of the body, and lower growth performance of the body, so as to achieve good feed effect, Improve digestibility and enhance the body's immunity

Pending Publication Date: 2021-08-31
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Black soldier fly powder drying and crushing equipment requires large investment and high energy consumption. High temperature treatment will destroy protein and high ash content, resulting in lower digestion and absorption rate of the body; in addition, feeding black soldier fly fresh worms whole or chopped, these are not denatured The protein may affect the digestion and absorption rate and reduce the growth performance of the body. Some small aquatic animals have small mouths and cannot directly eat the black soldier fly body. At the same time, the black soldier fly body has the risk of carrying viruses and bacteria. For different aquatic animals, black soldier fly The appropriate amount of adding fresh soldier flies varies; black soldier flies have different life cycles, black soldier fly larvae can be used for aquatic feeding, black soldier fly pupae or adults are difficult to use, so black soldier fly storage and transportation There are difficulties

Method used

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  • Processing method and application of fermented feed for aquatic products based on hermetia illucens
  • Processing method and application of fermented feed for aquatic products based on hermetia illucens
  • Processing method and application of fermented feed for aquatic products based on hermetia illucens

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Activation culture of bacillus, yeast and lactic acid bacteria:

[0038] A, first configure the culture medium of bacillus, saccharomyces and lactic acid bacteria;

[0039] Bacillus liquid and solid medium: tryptone 10g, sodium chloride 10g, yeast powder 5g, distilled water 1L, pH natural, sterilized at 121°C for 20min, the composition of the Bacillus solid medium is the same as that of the liquid medium; the difference is that 15g of agar is added .

[0040] Yeast liquid and solid medium: 200g potatoes boiled and filtered through 8 layers of gauze, glucose 20g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, distilled water to 1L, pH natural, sterilized at 121°C for 20min; The composition of the solid medium is the same as that of the liquid medium, the difference is that 15g of agar is added;

[0041] Lactic acid bacteria liquid and solid medium: beef extract 10g, tryptone 10g, glucose 20g, yeast powder 5g, dipotassium hydrogen phosphate 2g, diammoniu...

Embodiment 2

[0051] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;

[0052] (2) Configure the anaerobic fermentation medium, and the weight percentages of each component are respectively: black soldier fly homogenate 55% (water content 70%); bran 11.2%; rice bran 20%; secondary powder 13%; acidity Protease 0.8%, mixed uniformly to obtain anaerobic fermentation medium.

[0053] (3) Inoculate the fermented seed liquid of Bacillus natto, the fermented seed liquid of Saccharomyces cerevisiae, the fermented seed liquid of Lactobacillus plantarum, the seed liquid of Lactobacillus fermentum and the seed liquid of Lactobacillus rhamnosus after the activation culture of step (1) into the fermentation culture Base, the inoculum of Bacillus natto and Saccharomyces cerevisiae is 1.2% of the fermentation medium weight, the total number of live bacteria of Bacillus natto and Saccharomyces cerevisiae is 2.3 × 10 9 cfu / mL, the ino...

Embodiment 3

[0058] (1) the activation culture of bacillus, saccharomycete and lactic acid bacteria, concrete operation is with embodiment 1;

[0059] (2) Configure the anaerobic fermentation medium, and the weight percentages of each component are respectively: black soldier fly homogenate 48% (water content 70%); bran 20%; rice bran 14.7%; secondary powder 16%; acidity Protease 1.3%, mixed uniformly to obtain anaerobic fermentation medium.

[0060] (3) Inoculate the fermented seed liquid of Bacillus natto, the fermented seed liquid of Saccharomyces cerevisiae, the fermented seed liquid of Lactobacillus plantarum, the seed liquid of Bacillus coagulans and the seed liquid of Lactobacillus paracasei after the activation culture of step (1) into the fermentation medium , the inoculum of Bacillus natto and Saccharomyces cerevisiae is 1% of the weight of the fermentation medium, and the total number of live bacteria of Bacillus natto and Saccharomyces cerevisiae is 2.6 × 10 9 cfu / mL, the inoc...

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Abstract

The invention belongs to the field of biological feed, and specifically relates to a processing method and application of fermented feed for aquatic products based on hermetia illucens. The method comprises the following steps: activating a strain to obtain a bacillus fermentation seed solution, a saccharomycetes fermentation seed solution and a lactic acid bacteria fermentation seed solution; mincing and homogenizing fresh hermetia illucens larvae, and then mixing with bran, rice bran and wheat middling, adding acid protease, conducting mixing to obtain a fermentation culture medium, inoculating the bacillus fermentation seed solution, the saccharomycetes fermentation seed solution and the lactic acid bacteria fermentation seed solution, conducting anaerobic fermentation under the closed condition, and then obtaining a finished product. According to the present invention, fresh hermetia illucens are adopted for bacterium-enzyme synergistic fermentation, fermentation is thorough, and the finished product is high in small peptide content and rich in organic acid and has antibacterial activity; and meanwhile, the storage time is long, use is convenient, and good economic benefits are achieved.

Description

technical field [0001] The invention belongs to the field of biological feed, and in particular relates to a processing method and application of fermented feed for aquatic products based on black soldier flies. Background technique [0002] Aquafeed accounts for 40% to 60% of the cost of aquaculture. Fishmeal is one of the most important feed protein sources in aquaculture feed. More than 2 / 3 of fishmeal is used as aquafeed in the world, and it reaches about 60% in China. With the rapid development of the aquaculture industry, the problem of global fishmeal resource shortage is becoming more and more severe, and aquafeed relies on fishmeal and fish oil extracted from bait fish, resulting in an increase in the cost of aquaculture and seriously restricting the sustainable development of the aquaculture industry. Black soldier fly is a kind of insect of Diptera Soldier Fly family. It is widely distributed. Its larvae are rich in essential amino acids, among which the content o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/12A23K10/20A23K10/37A23K10/30A23K10/14
CPCA23K50/80A23K10/12A23K10/20A23K10/37A23K10/30A23K10/14Y02P60/87Y02A40/818
Inventor 陈华友康凌宇卢晨曦冯桃李柯仪倪忠高恶斌崔凤杰
Owner JIANGSU UNIV
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