Method for solid-state fermenting of soybean meal
A technology of solid-state fermentation and soybean meal, applied in the field of protein source fermentation, can solve the problems of reducing production cost, odor of soybean meal, increasing drying time, etc., and achieves the effects of simple production process equipment, improvement of environmental pollution, and promotion of digestion and absorption.
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Embodiment 1
[0021] A method for solid-state fermentation of soybean meal adopts the following steps:
[0022] (1) Selection of strains: select B. subtilis (B. subtilis) with strong protease production ability, lactic acid bacteria (L. plantarum) and yeast (S. cerevisiae) with strong acid production ability,
[0023] Bacillus subtilis was purchased from the China Industrial Microbial Culture Collection and Management Center, address: Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing, the strain number is CICC NO.10071, and the lactic acid bacteria were purchased from the China Industrial Microbial Culture Collection and Management Center , The strain number is CICC NO.6009, the yeast is purchased from the China Industrial Microorganism Collection and Management Center, and the strain number is CICC NO.1421;
[0024] (2) Preparation of seed liquid:
[0025] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glyceri...
Embodiment 2
[0030] A method for solid-state fermentation of soybean meal adopts the following steps:
[0031] (1) The selection of strains is the same as in Example 1;
[0032] (2) Preparation of seed liquid:
[0033] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glycerin tube to the corresponding seed culture medium, in which the Bacillus subtilis is inoculated into the LB medium, cultured at 37℃, 180rpm for 24h, and the yeast is inoculated Into the potato culture medium, cultivate for 24h at 30℃, 200rpm, inoculate the lactic acid bacteria into the MRS medium, culture at 37℃ for 24h,
[0034] (2-2) Preparation of secondary seed liquid: inoculate the above-mentioned primary seeds into a seed fermenter, inoculate lactic acid bacteria into a 10L glass bottle, and ferment at 37°C for 24h; inoculate yeast into a 15L fermentor at 200 rpm / min, ventilation 1m 3 / h, keep positive pressure and ferment for 24h at 30℃; inoculate Bacillus ...
Embodiment 3
[0038] A method for solid-state fermentation of soybean meal adopts the following steps:
[0039] (1) The selection of strains is the same as in Example 1;
[0040] (2) Preparation of seed liquid:
[0041] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glycerin tube to the corresponding seed culture medium, in which the Bacillus subtilis is inoculated into the LB medium, cultured at 37℃, 180rpm for 24h, and the yeast is inoculated Into the potato culture medium, cultivate at 30℃, 200rpm for 30h, inoculate the lactic acid bacteria into MRS culture medium, culture at 37℃ for 30h,
[0042] (2-2) Preparation of secondary seed liquid: inoculate the above-mentioned primary seeds into a seed fermenter, inoculate lactic acid bacteria into a 10L glass bottle, and ferment at 37°C for 30h; inoculate yeast into a 15L fermentor at 200 rpm / min, ventilation 1m 3 / h, keep positive pressure, ferment for 30h at 30℃; inoculate Bacillus...
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