Method for solid-state fermenting of soybean meal

A technology of solid-state fermentation and soybean meal, applied in the field of protein source fermentation, can solve the problems of reducing production cost, odor of soybean meal, increasing drying time, etc., and achieves the effects of simple production process equipment, improvement of environmental pollution, and promotion of digestion and absorption.

Inactive Publication Date: 2016-12-07
上海邦成生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to reports, the research field of microbial fermentation of soybean meal is often to use a protease-producing strain to simply degrade the crude protein in soybean meal into small peptides. Disadvantages such as low flavor and less probiotics
[0003] Chinese patent CN104054902A discloses a process for producing fermented soybean meal by solid-state fermentation of mixed strains. The self-made breeding medium can be used to breed and cultivate four strains at the same time, and then ferment the obtained bacterial liquid on soybean meal. The process of breeding and fermentation Less, easy to operate and achieve large-scale production, the fermented soybean meal has low water content, loose and not sticky, short drying time, which reduces production costs, and drying at a lower temperature can keep the probiotics in it biologically active, However, the drying temperature of the fermentation in this patent is 55°C, which will greatly increase the drying time, thereby increasing the production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for solid-state fermentation of soybean meal adopts the following steps:

[0022] (1) Selection of strains: select B. subtilis (B. subtilis) with strong protease production ability, lactic acid bacteria (L. plantarum) and yeast (S. cerevisiae) with strong acid production ability,

[0023] Bacillus subtilis was purchased from the China Industrial Microbial Culture Collection and Management Center, address: Building 6, No. 24, Jiuxianqiao Middle Road, Chaoyang District, Beijing, the strain number is CICC NO.10071, and the lactic acid bacteria were purchased from the China Industrial Microbial Culture Collection and Management Center , The strain number is CICC NO.6009, the yeast is purchased from the China Industrial Microorganism Collection and Management Center, and the strain number is CICC NO.1421;

[0024] (2) Preparation of seed liquid:

[0025] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glyceri...

Embodiment 2

[0030] A method for solid-state fermentation of soybean meal adopts the following steps:

[0031] (1) The selection of strains is the same as in Example 1;

[0032] (2) Preparation of seed liquid:

[0033] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glycerin tube to the corresponding seed culture medium, in which the Bacillus subtilis is inoculated into the LB medium, cultured at 37℃, 180rpm for 24h, and the yeast is inoculated Into the potato culture medium, cultivate for 24h at 30℃, 200rpm, inoculate the lactic acid bacteria into the MRS medium, culture at 37℃ for 24h,

[0034] (2-2) Preparation of secondary seed liquid: inoculate the above-mentioned primary seeds into a seed fermenter, inoculate lactic acid bacteria into a 10L glass bottle, and ferment at 37°C for 24h; inoculate yeast into a 15L fermentor at 200 rpm / min, ventilation 1m 3 / h, keep positive pressure and ferment for 24h at 30℃; inoculate Bacillus ...

Embodiment 3

[0038] A method for solid-state fermentation of soybean meal adopts the following steps:

[0039] (1) The selection of strains is the same as in Example 1;

[0040] (2) Preparation of seed liquid:

[0041] (2-1) Preparation of the first-level seed solution: inoculate the bacteria preserved in the inclined plane or the glycerin tube to the corresponding seed culture medium, in which the Bacillus subtilis is inoculated into the LB medium, cultured at 37℃, 180rpm for 24h, and the yeast is inoculated Into the potato culture medium, cultivate at 30℃, 200rpm for 30h, inoculate the lactic acid bacteria into MRS culture medium, culture at 37℃ for 30h,

[0042] (2-2) Preparation of secondary seed liquid: inoculate the above-mentioned primary seeds into a seed fermenter, inoculate lactic acid bacteria into a 10L glass bottle, and ferment at 37°C for 30h; inoculate yeast into a 15L fermentor at 200 rpm / min, ventilation 1m 3 / h, keep positive pressure, ferment for 30h at 30℃; inoculate Bacillus...

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PUM

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Abstract

The invention relates to a method for solid-state fermenting of soybean meal. The method comprises four steps such as strain selection, seed liquid preparation, fermentation broth preparation and solid-state fermentation. Compared with the method in the prior art, the method of the present invention has the following characteristics that solid-state fermentation is performed on soybean meal by combining Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus plantarum; during the fermentation process, the Bacillus subtilis secretes protease to degrade crude protein into small peptides, a large amount of the Saccharomyces cerevisiae grow to synthesize bacterial protein, and a large amount of the Lactobacillus plantarum breed to secrete organic acids so as to improves the flavor of the fermented soybean meal, improve the nutritive value, promote the digestion and absorption of the nutrients; and according to the fermented product, the feeding quality of the fermented soybean meal and the efficiency of the fermented soybean meal are substantially improved, and the characteristics of remarkable economy, remarkable environmental protection and high efficiency are provided.

Description

Technical field [0001] The invention relates to a protein source fermentation method, in particular to a method for solid-state fermentation of soybean meal. Background technique [0002] Soybean meal is a high-quality protein source that is currently used by the livestock industry. However, soybean meal contains a large amount of proteinogen and anti-nutritional factors, which seriously affect the digestion and absorption of soybean meal protein by animals. By hydrolyzing soybean meal protein into soybean meal peptides, it can greatly improve the animal's absorption and utilization rate. At present, soybean meal processing and production methods include enzymatic hydrolysis, physical and chemical methods, and fermentation methods. Enzymatic hydrolysis uses the characteristics of enzymatic directional decomposition to decompose macromolecular proteins into small peptides, but the peptides produced by enzymatic hydrolysis are more bitter and have poor palatability; physical and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/00
Inventor 郑锐郑云峰陆克文
Owner 上海邦成生物工程有限公司
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