Preparation method of high-activity ocean rhodotorula glutinis powder

Abstract

The invention relates to a new method for preparing high-activity ocean rhodotorula glutinis powder. The method comprises the following steps: beer yeast is taken as a raw material and hydrolyzed by hydrolase to be a basal medium after filtering; after ocean rhodotorula glutinis is inoculated at deep fluid for aerobic culture, and an ultrafiltration membrane is utilized in time to concentrate fermentation liquor and collect rhodotorula glutinis thallus; and the concentrated rhodotorula glutinis is mixed evenly with excipient to form wet cenobium, and then the wet cenobium are transmitted to expansion drying equipment by a screw stem for instantaneous drying at low temperature to obtain the rhodotorula glutinis dry powder of which the rhodotorula glutinis thallus reaches 10-20 billion / g, and the viable yeast content reaches above 60%. The rhodotorula glutinis prepared by the preparation method of the invention has high growth speed, high density of yeast as per unit volume and short incubation time; the ultrafiltration membrane is adopted to concentrate culture solution, which has small damage to thallus and easily keeps high activity of the concentrated rhodotorula glutinis; granulation is not needed after the concentrated rhodotorula glutinis solution and the excipient are mixed; and the obtained wet cenobium can directly enter the expansion drying equipment to for instantaneous drying at low temperature, thus ensuring high activity ratio of the rhodotorula glutinis powder in the whole production technology process.

Description

Technical field [0001] The invention relates to a preparation method of marine red yeast. Specifically, it relates to a method for preparing highly active marine red yeast powder with short culture period and high active cell ratio. Background technique [0002] Marine red yeast (Rhodotorula sp.) is a Phaffia rhodozyma strain isolated from marine soil. The bacterial body is rich in protein, glycogen, unsaturated fatty acid, animal larval growth hormone, and carotenoids based on astaxanthin. (It has a strong ability to scavenge free radicals, promote antibody production, enhance host immunity, and bright red color). Therefore, marine red yeast is a potential astaxanthin-producing strain and a very good biological feed for aquatic animal larvae. Marine red yeast can also resurrect, grow and reproduce. The growth and metabolism of red yeast can purify harmful substances such as ammonia nitrogen and hydrogen sulfide in the water. Active marine red yeast is used in the healthy and la...

AI Technical Summary

Problems solved by technology

[0003] Chinese patent application CN1146290A discloses a "fresh single-cell marine red yeast ecological bait and its production method", the invention involves a marine red yeast isolated from nature, a feeding liquid fermentation, post-processing to make liquid products The method has the disadvantages of long production cycle of marine red yeast, low cell concentration of fermented marine red yeast, liquid products need to be refrigerated, etc., which are not conducive to large-scale production, long-term storage and long-distance transportation

This method has a long cultivation cycle and needs 6 to 8 days. Long-term aeration cultivation is easy to contaminate, and the solid red yeast produced by this method is an inactive cell.

Chinese patent ZL02139178.5 discloses "A Starch Adsorption Fluidized Bed Drying Production Method for Solid Marine Red Yeast", the solid red yeast produced by this method has a living cell ratio of only 15% to 30%, and the active cell ratio is low The problem