Preparation method of high-activity ocean rhodotorula glutinis powder

A marine red yeast, high-activity technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of easy pollution, long production cycle, no activity, etc., and achieve fast growth rate and bacterial damage. The effect of small, high active ratios

Inactive Publication Date: 2010-06-23
DALIAN RES & DESIGN INST OF CHEM IND
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent application CN1146290A discloses a "fresh single-cell marine red yeast ecological bait and its production method", the invention involves a marine red yeast isolated from nature, a feeding liquid fermentation, post-processing to make liquid products The method has the disadvantages of long production cycle of marine red yeast, low cell concentration of fermented marine red yeast, liquid products need to be refrigerated, etc., which are not conducive to large-scale producti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The marine red yeast strains stored on the PYG nutrient agar slant were retransmitted to the slant and cultured at 30°C for 24h, then the slant strains were eluted and inoculated in 10L PYG nutrient solution at 30°C for 24h, and then connected to 300L marine red yeast In the base seed pot, aerate and cultivate at 30°C for 24 hours to obtain the production seed liquid.

[0031] Take 1200L of fresh beer yeast slurry with 15% solid content, heat up the jacket to 50℃, add neutral protease to hydrolyze for 2h, add 5000L tap water to cool, filter through plate and frame filter press to obtain 6000L filtrate, add 3% glucose , Potassium dihydrogen phosphate 0.3%, sodium chloride 0.5%, adjust the pH to 6.5. Sterilize the medium in a 10t fermentor at 121°C for 30 minutes, insert 300L of red yeast seed liquid, and cultivate at 30-32°C for 20 hours with aeration to obtain 3 billion / mL fresh marine red yeast fermentation broth. The cultured marine red yeast was subjected to ultrafiltr...

Embodiment 2

[0034] After cultivating the newly introduced sloping surface of the marine red yeast strain at 30℃ for 24h, the slant strain was eluted and inoculated in 10L PYG nutrient solution at 30℃ for 24h, and then connected to the seed tank containing 300L marine red yeast production medium. Cultivation with aeration at ℃ for 20h to obtain production seed liquid.

[0035] Take 300kg of dry brewer's yeast powder, add 900kg of water and stir evenly to obtain 25% brewer's yeast slurry. The jacket is heated to 40℃, hydrolyzed by alkaline protease for 1.5h, 6000L of tap water is cooled and diluted, and filtered through a plate and frame filter press. 6600L of filtrate, add glucose 4%, potassium dihydrogen phosphate 0.35%, sodium chloride 0.5%, and adjust the pH to 6.0. Sterilize in a 10t fermentor at 121°C for 30 minutes, and cultivate at 30-32°C for 20 hours with aeration to obtain 3.5 billion / mL marine red yeast fermentation broth. The cultured marine red yeast was subjected to ultrafiltra...

Embodiment 3

[0038] After cultivating the new sloping surface of the marine red yeast strain at 30°C for 24h, the sloping surface strains are eluted and inoculated in 10L PYG nutrient solution at 30°C for 24h, and then connected to the seed tank containing 300L marine red yeast production medium , 28 ℃ ventilated culture for 24 hours to obtain production seed liquid.

[0039] Take 350kg of dry brewer's yeast powder, add 1100kg of water and stir evenly to obtain brewer's yeast slurry, heat the jacket to 40℃, add alkaline protease to hydrolyze for 1.5h, add 5000L of tap water to cool and dilute, filter by plate and frame filter to obtain 5600L filter For the clear solution, add 5% glucose, 0.4% potassium dihydrogen phosphate, and 0.5% sodium chloride to adjust the pH to 7.0. Sterilize in a 10t fermenter at 115°C for 60 minutes, and cultivate at 28-30°C for 24 hours with aeration to obtain 4 billion / mL marine red yeast fermentation broth. The cultured marine red yeast was subjected to ultrafilt...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a new method for preparing high-activity ocean rhodotorula glutinis powder. The method comprises the following steps: beer yeast is taken as a raw material and hydrolyzed by hydrolase to be a basal medium after filtering; after ocean rhodotorula glutinis is inoculated at deep fluid for aerobic culture, and an ultrafiltration membrane is utilized in time to concentrate fermentation liquor and collect rhodotorula glutinis thallus; and the concentrated rhodotorula glutinis is mixed evenly with excipient to form wet cenobium, and then the wet cenobium are transmitted to expansion drying equipment by a screw stem for instantaneous drying at low temperature to obtain the rhodotorula glutinis dry powder of which the rhodotorula glutinis thallus reaches 10-20 billion/g, and the viable yeast content reaches above 60%. The rhodotorula glutinis prepared by the preparation method of the invention has high growth speed, high density of yeast as per unit volume and short incubation time; the ultrafiltration membrane is adopted to concentrate culture solution, which has small damage to thallus and easily keeps high activity of the concentrated rhodotorula glutinis; granulation is not needed after the concentrated rhodotorula glutinis solution and the excipient are mixed; and the obtained wet cenobium can directly enter the expansion drying equipment to for instantaneous drying at low temperature, thus ensuring high activity ratio of the rhodotorula glutinis powder in the whole production technology process.

Description

Technical field [0001] The invention relates to a preparation method of marine red yeast. Specifically, it relates to a method for preparing highly active marine red yeast powder with short culture period and high active cell ratio. Background technique [0002] Marine red yeast (Rhodotorula sp.) is a Phaffia rhodozyma strain isolated from marine soil. The bacterial body is rich in protein, glycogen, unsaturated fatty acid, animal larval growth hormone, and carotenoids based on astaxanthin. (It has a strong ability to scavenge free radicals, promote antibody production, enhance host immunity, and bright red color). Therefore, marine red yeast is a potential astaxanthin-producing strain and a very good biological feed for aquatic animal larvae. Marine red yeast can also resurrect, grow and reproduce. The growth and metabolism of red yeast can purify harmful substances such as ammonia nitrogen and hydrogen sulfide in the water. Active marine red yeast is used in the healthy and la...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/16C12R1/645C12R1/865
Inventor 温朗聪张宾红
Owner DALIAN RES & DESIGN INST OF CHEM IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap