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Production method of amino acid bioactive peptide for aquatic products

A production method, amino acid technology, applied in microorganism-based methods, biochemical equipment and methods, applications, etc., can solve problems such as water shortage, protein shortage, and brand shortage.

Inactive Publication Date: 2016-08-17
闫大凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the problems faced by the aquaculture and feed industry in our country are: lack of land, water, protein and brand

Method used

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  • Production method of amino acid bioactive peptide for aquatic products
  • Production method of amino acid bioactive peptide for aquatic products
  • Production method of amino acid bioactive peptide for aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] (1) Preparation of mint paste: Preparation of mit paste:

[0091] Clean the purchased fresh fish to remove impurities, pour it into a fish paste grinder, grind it into a paste, pour the paste fish paste into a closed container, and steam it for aging and sterilization. The technical parameters of the aging process are: aging pressure: 0.5-0.8MPa, aging time: 15-18 minutes, after the pressure is released, fish oil and fish paste are separated, and after separation, the fish paste is centrifuged online in a centrifuge, and the centrifugal speed is : 1000-1200 rpm, the large particles at the bottom are fish meal, and the upper part is fish essence paste. The water content of milt extract is 30%-40%; (2) Preparation of Bacillus subtilis seed liquid:

[0092] 1) Single colony isolation: spread the activated Bacillus subtilis slant strain on a petri dish filled with newly prepared glucose beef extract peptone medium, and culture it in a constant temperature incubator at 30°C...

Embodiment 2

[0137] Amino acid active peptides (test group 2) and amino acid active peptides (control group) obtained in embodiment 1 are used in special aquatic feeds to replace fish meal and soybean meal in half amount in grouper, and observe and measure the effect of test on feed rate of return ( FCR), survival rate and the influence of weight gain rate effect, experimental design and test result are shown in Table 4 and Table 5:

[0138] Table 4. Experimental design of half-quantity replacement of fish meal and soybean meal with amino acid active peptides used in aquatic products in grouper

[0139]

[0140] *Basic ingredient amount: The amount of protein is not included in the ingredients. Basic protein amount: including fish meal and soybean meal;

[0141] Table 5. Effects of semi-replacing fish meal and soybean meal with amino acid active peptides in aquatic products on weight gain and survival rate of grouper (30 days)

[0142]

[0143]

[0144]As can be seen from Table ...

Embodiment 3

[0146] The amino acid active peptide for aquatic products obtained in Example 1 is used in special aquatic feeds to replace fish meal and soybean meal in half, and the application test is carried out in Penaeus vannamei, and the effects of the determination test on feed reward ratio (FCR), survival rate and weight gain rate are observed. The impact of the test design and test results are shown in Table 6, Table 7 and Table 8, Table 9, Table 10:

[0147] Table 6. Experimental design of half-quantity replacement of fish meal and soybean meal with amino acid active peptides for aquatic products on Penaeus vannamei

[0148]

[0149] *Basic ingredient amount: The amount of protein is not included in the ingredients. Basic protein amount: including fish meal and soybean meal;

[0150] Table 7. Effects of special aquatic feed containing amino acid active peptides on the survival rate and weight gain of Penaeus vannamei (30 days)

[0151]

[0152] *Water temperature 26°C;

[...

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Abstract

The invention relates to a microbial fermentation technology and a production technology of a special aquatic feed, in particular to a production method for producing an amino acid bioactive peptide by a microbial fermentation method. Milt paste, soybean meal, peanut meal and dried corn steep liquor powder are used as fermentation raw materials; bacillus subtilis, bacillus natto, saccharomyces cerevisiae, rhodotorula benthica and lactobacillus acidophilus are used as microbial fermentation communities; five microorganism liquid seeds respectively adopt seed cultivation of a shaking flask and fermentation cultivation of a fermentation tank. After solid and liquid are uniformly mixed according to a proportion, the mixture is placed on a fermentation bed and is subjected to aerobic solid fermentation by filling in sterile air; then by drying, grinding and sieving, the amino acid bioactive peptide for the aquatic products is obtained. The production method has the characteristics of wide raw material source, mature fermentation process, high protein content of the product, reasonable amino acid component, high peptide conversion rate, more enzyme products, good attractant effect and the like. When the amino acid bioactive peptide is applied to aquatic livestock such as marine and freshwater fishes, shrimps, crabs, spectacular fish stock and the like, diseases can be prevented and treated, a survival rate and an immunity are improved, a growth speed and quality are improved, the breeding environment also can be improved, and water quality is purified; the amino acid bioactive peptide for the aquatic products is a green functional feed additive.

Description

technical field [0001] The invention relates to microbial fermentation technology and production technology of special aquatic feed, in particular to an amino acid active peptide for aquatic products produced by microbial fermentation. Background technique [0002] my country is the largest aquaculture country in the world, and its aquaculture production accounts for more than 70% of the world's total. In 2015, the total output of aquatic products in my country reached 66 million tons, including 56 million tons of aquaculture, 30 million tons of freshwater fish and 26 million tons of seawater fish. With the change of people's dietary structure, the consumption of aquatic products is increasing rapidly, and the position of aquaculture in global consumption is becoming more and more important. At present, my country's aquatic feed industry has entered a period of rapid and steady development. The total output of farmed fish and shrimp in my country is about 26 million tons, ...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P21/00A23K20/147C12R1/125C12R1/07C12R1/865C12R1/645C12R1/23
CPCC12P39/00C12P21/00
Inventor 闫大凤葛明巧
Owner 闫大凤
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