Oceanic red yeast solid-fermentation method
A technology of marine red yeast and solid fermentation, applied in the direction of fungi, etc., can solve the problems of high cost of liquid fermentation and low bacterial content
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[0013] (1) Inoculate the marine Rhodotorula sp. strains in YPD liquid medium, and shake culture for 48 hours as the fermentation seed liquid; the culture temperature is 30°C, and the rotation speed is 150rpm;
[0014] (2) Inoculate the activated fermentation seed liquid in step (1) into the fermentor for 48 hours with stirring and aeration fermentation; the culture medium is: glucose 15g / L, peptone 10 g / L, yeast powder 10 g / L, magnesium sulfate 0.5 g / L, pH 5.8, the rest is seawater, sterilize 15min under 0.1MPa. Cultivation conditions: temperature of 30 degrees; stirring speed of 180rpm / min, aeration rate of 2m3 / min;
[0015] (3) 40% bran, 30% soybean meal, 15% lotus root starch, 15% corn starch are configured as a solid medium; sucrose 5g / L, peptone 5g / L, yeast powder 2 g / L, magnesium sulfate 0.5 g / L, Add to seawater and configure it as a liquid medium; mix the solid medium and liquid medium at a weight ratio of 1:1 and configure it as a solid medium; load the solid medium into a...
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