High-efficiency pig feed additive
A feed additive and compound polysaccharide technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of low absorption rate, high excretion rate and poor compatibility of pigs, and achieve good compatibility, low excretion rate and little environmental pollution. Effect
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Embodiment 1
[0032] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance
[0033] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and culture. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator.
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Embodiment 2
[0050] Example 2: (Comparison Test) Research on Fermentation Conditions of High-yielding Carotenoids of Rhodotorula marina WYN1
[0051] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , the inoculum amount was 10%, the shaker speed was 180r / min, and after culturing at 28°C for 24 hours, the temperature was adjusted to 30°C, the pH was adjusted to 5, and the nitrogen source was added once, that is, peptone was added according to the addition amount of 2.5%, and continued Ferment for 24h. The marine Rhodotorula WYN1 was fermented and cultured by the above method, and its biomass, carotenoid production and intracellular copper content were 27.52 g / L, 25.12 mg / L and 6240 μg / g dry bacteria, respectively.
Embodiment 3
[0052] Embodiment 3: feed additive and its preparation
[0053] The parts by weight of the feed additive are composed as follows:
[0054] Compound polysaccharide extract 10, marine red yeast fermented culture 15, epimedium extract 6, licorice 5, hawthorn 5, honeysuckle 2, dandelion 3; the marine red yeast preservation number is CCTCCNO: M2014592.
[0055] The preparation method of described complex polysaccharide extract is as follows:
[0056] Dry and crush the raw materials astragalus, wolfberry, and alfalfa with a mass ratio of 4:2:1, pass through a 60-mesh sieve, add boiling water 8 times the total weight of the crushed matter, extract twice for 30 minutes each time, filter and mix the extracts, and naturally cool to room temperature , adding absolute ethanol to the extract solution to make the ethanol concentration in the mixture reach 70%, airtightly refrigerating and precipitating at 10°C for 8 hours, drying the precipitate in vacuum, pulverizing and passing through a...
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