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Feed additive product

A technology of feed additive and marine red yeast, which is applied in animal feed, animal feed, application, etc., can solve the problems of low absorption rate of pigs, pollute the environment, and high excretion rate, and achieve good compatibility, low environmental pollution, and low excretion rate Effect

Inactive Publication Date: 2015-11-11
天津天绿健科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many problems in adding this inorganic copper sulfate to the feed, such as low absorption rate of pigs, poor compatibility with other components in the diet, high excretion rate, and environmental pollution.

Method used

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  • Feed additive product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Acquisition of marine Rhodotorula yeast WYN1 and domestication of copper tolerance

[0035] (1) Fresh sea shrimp, starfish, seaweed and other biological samples collected from the Yellow Sea are placed in sterile YEPD medium, and red or pink round colonies are picked for further purification and cultivation. According to the accumulation of carotenoids in each strain, the strains with high carotenoid production were screened. Among them, the marine rhodotorula WYN1 with higher carotenoid production has oval cells without pseudohyphae; it forms red colonies on the YEPD medium prepared in seawater, with smooth surface and neat edges; it does not produce eggs on the sporulation medium Cystic spores, multilateral budding, no sporulation; no fermentation of sugar, no formation of starch-like compounds, and no inositol synthesis. Based on the above characteristics, it was preliminarily determined to belong to the genus Rhodotorula. Keep ramps in the refrigerator....

Embodiment 2

[0052] Example 2: (Comparative Test) Fermentation Conditions for High-yielding Carotenoids of Rhodotorula marina WYN1

[0053] Fermentation medium composition: glucose 2.5%, peptone 2.5%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.15%, copper content 300mg / L (copper sulfate configuration), sodium chloride 20%, the rest is water; the initial pH is 8 , the inoculum amount was 10%, the shaker speed was 180r / min, and after culturing at 28°C for 24 hours, the temperature was adjusted to 30°C, the pH was adjusted to 5, and the nitrogen source was added once, that is, peptone was added according to the addition amount of 2.5%, and continued Ferment for 24h. The marine Rhodotorula WYN1 was fermented and cultured by the above method, and its biomass, carotenoid production and intracellular copper content were 27.52 g / L, 25.12 mg / L and 6240 μg / g dry bacteria, respectively.

Embodiment 3

[0054] Embodiment 3: feed additive preparation

[0055] The composition of a kind of feed additive is as follows:

[0056] 15% fructooligosaccharides, 10% propolis, 25% Ligustrum lucidum extract, 15% fermented culture of Rhodotorula lucidum, 35% solid fermented culture of Ganoderma lucidum; the preservation number of Rhodotorula marina is CCTCCNo: M2014592, and the above ratios are for mass percentage.

[0057] FOS is a commercially available product.

[0058] The preparation method of ganoderma lucidum solid fermentation culture is as follows:

[0059] (1) Activation culture of slant strains: transfer the preserved ganoderma lucidum slant strains to the slant medium, and cultivate at 25°C for about 110 hours until the mycelia cover the slope;

[0060] (2) Liquid first-level seed culture: pick a piece of the above-mentioned Ganoderma lucidum slant strain and insert it into a 500-milliliter shaker flask equipped with 100 milliliters of medium to carry out first-level seed cu...

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Abstract

The invention provides a feed additive product. The feed additive product is composed of the following components: 10%-15% of fructo-oligosaccharide, 10%-20% of propolis, 10%-25% of glossy privet fruit extract, 15%-30% of ocean rhodotorula fermentation culture and 30%-50% of ganoderma lucidum solid fermentation culture, wherein the preservation number of the ocean rhodotorula fermentation culture is CCTCC No:M 2014592, and the ratios are mass percent. According to the feed additive product, ocean rhodotorula can produce carotenoid with high yield and also can gather copper; the carotenoid yield and copper content of a yeast are effectively improved through a static cultivation method; the ocean rhodotorula is applied to a feed as a feed additive and can provide the carotenoid, group-B vitamins and copper ions; the ocean rhodotorula can replace inorganic copper ions used in conventional feed due to the efficient copper gathering capability; the product has good stability, good compatibility with other components in the feed, high absorption rate, low excretion rate and small environmental pollution, can be used for improving the skin complexions and hair colors of animals, and enhancing the nutritive value of the feed.

Description

technical field [0001] The invention belongs to the field of feed, in particular to a feed additive product and a preparation method thereof. Background technique [0002] In the process of modern livestock and poultry farming, in order to prevent and treat animal diseases, excessive antibiotic products are often used to ensure the health of animals, but the excessive use of antibiotics often leads to the decline in the quality of animal meat products and their products and the enhancement of antibiotic resistance, resulting in transmission through the food chain. Human health is also severely affected by the problem of antibiotics. [0003] Some natural substances derived from nature not only have good nutritional properties, but also have anti-virus, anti-inflammatory, anti-oxidation, and regulation of immune functions, etc., and have broad application prospects. [0004] The main components of propolis are flavonoids, phenols, lactones, coumarin, aldehydes and ketones, a...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/14A23K1/00
Inventor 李建树
Owner 天津天绿健科技有限公司
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