Addition agent for maintaining activity of probiotics, compound probiotics and preparation method of compound probiotics

A technology of composite probiotics and additives, applied in the direction of bacteria, applications, and lactobacilli used in food preparation, can solve the problems of improving the activity of probiotics and rarely considering the protective effect of additives and probiotics, so as to promote proliferation and growth, Low cost, thickening effect

Active Publication Date: 2021-06-04
广州莱可福生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Downstream enterprises of probiotics (manufacturers of various dosage forms of probiotics) have been unable to increase the activity of probiotics from the source. Generally, the inactivation of probiotics is reduced by improving the processing technology, and the use of auxiliary materials improves the transport of probiotics through the upper and lower digestive tracts. Survival rate of probiotics, but little consideration is given to the protective effect of additives on probiotics during production, transportation and product shelf life, because no additives with this effect have been found so far

Method used

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  • Addition agent for maintaining activity of probiotics, compound probiotics and preparation method of compound probiotics
  • Addition agent for maintaining activity of probiotics, compound probiotics and preparation method of compound probiotics

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Embodiment 1

[0038] (1) The present invention is a combination of whey protein and inulin selected through a large number of experiments. The freeze-dried powder obtained by other combinations has poor dispersibility in water, or poor dispersibility in part. When brewing, it sinks to the bottom and is not easy to drink, and the powdery feeling will be more obvious. The preparation method of compound probiotics is described in detail below: additive for preparing polywhey protein-inulin freeze-dried powder: dissolving whey protein powder in water to form 6% (w / v specific unit such as g / 100mL×100%) whey protein solution; inulin was dissolved in water to form a 20% (w / v, supra) solution. Stir and heat the whey protein solution at 90°C for 20 minutes, add an appropriate amount of inulin solution to the heated polymerized whey protein solution in a hot state, so that the ratio of whey protein to inulin is 3:1, and the polymerized whey protein The protein-inulin solution continued to stir and h...

Embodiment 2

[0044] (1) Prepare polywhey protein-inulin freeze-dried powder: whey protein powder is dissolved in water to form 15% (w / v) whey protein solution; inulin is dissolved in water to form 20% (w / v) )The solution. Stir and heat the whey protein solution at 70°C for 10 minutes, add an appropriate amount of inulin solution to the heated polymerized whey protein solution in a hot state, so that the ratio of whey protein to inulin is 6:1, and the polymerized whey protein -Continue stirring and heating the inulin solution for 10 minutes, then cool to room temperature to obtain a weak gel. Freeze the weak gel at -60°C for 18 hours, then freeze-dry and crush at low temperature to a particle size of 0.1 mm to obtain the polywhey protein-inulin freeze-dried powder.

[0045] (2) According to parts by weight, take 5 parts of Bifidobacterium longum bacteria powder (the live bacteria content is 5×10 10 CFU / g), 5 parts of Lactobacillus acidophilus powder (the live bacteria content is 1×10 11 ...

Embodiment 3

[0050] (1) Prepare polywhey protein-inulin freeze-dried powder: whey protein powder is dissolved in water to form 10% (w / v) whey protein solution; inulin is dissolved in water to form 20% (w / v) )The solution. Stir and heat the whey protein solution at 80°C for 15 minutes, add an appropriate amount of inulin solution to the heated polymerized whey protein solution in a hot state, so that the ratio of whey protein to inulin is 4:1, and the polymerized whey protein -Continue stirring and heating the inulin solution for 15 minutes, then cool to room temperature to obtain a weak gel. Freeze the weak gel at -40°C for 24 hours, then freeze-dry and crush at low temperature to a particle size of 0.3 mm to obtain the polywhey protein-inulin freeze-dried powder.

[0051] (2) According to parts by weight, weigh Bifidobacterium animalis, Bifidobacterium adolescentis, Bifidobacterium infantis, Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus rhamnosus compound lactic acid bact...

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Abstract

The invention provides an addition agent for maintaining activity of probiotics, compound probiotics and a preparation method of compound probiotics. The addition agent is obtained by performing heat treatment, freezing treatment and freeze drying treatment on whey protein and inulin. The compound probiotics are prepared from, by weight in parts, 10-30 parts of probiotic powder, 40-60 parts of the addition agent and 10-50 parts of water-soluble dietary fiber. Through the provided addition agent, the activity of probiotics can be better maintained during production and transportation and within product preservation periods. When the provided compound probiotics pass through gastrointestinal tracts, the powerful adsorption capacity of polymerized whey protein-inulin freeze-dried powder can wrap the probiotic powder, so that the resistance of probiotics to stomach acid and bile can be realized; and simulated gastric juice and intestinal juice tolerance tests and simulated bile salt tolerance tests show that the probiotics can maintain higher viable bacteria survival rates, and the viable bacteria survival rates can reach more than 90%.

Description

technical field [0001] The invention belongs to the technical field of food additives, and in particular relates to a compound probiotic and a preparation method thereof. Background technique [0002] At present, it is increasingly recognized that probiotics play a key role in human health. Probiotics are generally considered to be active microorganisms that have positive benefits to the host (such as animals or humans) after ingestion, and can improve the micro-ecological balance of the human body. With the increasingly widespread application of probiotics, they are used in various dosage forms, such as powders, granules, granules, capsules, liquid milk, beverages, tablets and oral liquids, etc., while oral intake is currently the most important way to supplement probiotics. The most common method. Production includes strain selection, production environment, selection of excipients, production process, packaging, etc., for sale on the shelf, and after eating, it passes t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L33/21A23P10/47
CPCA23L33/135A23L33/21A23P10/47A23V2002/00A23V2400/125A23V2400/113A23V2400/175A23V2400/513A23V2400/515A23V2400/529A23V2400/533A23V2200/3204A23V2200/3202A23V2250/54252A23V2250/5062A23V2250/606A23V2250/638A23V2250/6412A23V2250/5114A23V2250/632A23V2250/602
Inventor 钟伟杰张献苏芳萍黄冬明
Owner 广州莱可福生物科技有限公司
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