Method for enhancing petroleum pollution removing effect of seawater
A CNPC and seawater technology, which is applied in the field of improving the removal effect of oil pollution in seawater, can solve problems such as water environment and aquatic organism damage, and achieve the effects of reducing treatment costs, safety in production process, and improving removal rate.
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Embodiment 1
[0027] In this example, high biosurfactant-producing strains and high-efficiency oil-degrading strains were respectively inserted into liquid medium for fermentation and activation. Take 1 mL of the fermentation broth of high-yielding biosurfactant bacteria and high-efficiency petroleum-degrading bacteria, respectively, and insert them into 90 mL of liquid medium, and carry out mixed culture in a shaker for 48 hours. Then add 1 g of chitin and 2 g of mannitol extracted from shrimp shells, and continue culturing for 24 hours. After the mixed bacterial solution was frozen for 3 days and dried for 3 days, 2.0 g of bacterial powder was made.
Embodiment 2
[0029] In this example, 0.55 g of chitin extracted from shrimp shells, 20 mL of high-production biosurfactant bacterial liquid, and 20 mL of high-efficiency petroleum-degrading bacterial liquid were used to prepare 1.0 g of bacterial powder. Simulate 100mL of seawater polluted by oil, the initial concentration of oil is 1700mg / L, and the salinity of seawater is 3%. Sprinkle 1.0g of bacterial powder into a conical flask filled with 100mL of simulated oil-polluted seawater, then put the conical flask into a shaker, the temperature of the shaker is controlled at 25°C, and the time for bacterial powder to treat oil-polluted seawater is 1d . Small test results: the degradation rate of total petroleum hydrocarbons in simulated seawater is about 50%, and the concentration of remaining total petroleum hydrocarbons in seawater is 845mg / L, which is much higher than the second grade in the "Discharge Standard for Oily Wastewater from Offshore Oil Development Industry" standard.
Embodiment 3
[0031] In this example, 0.55 g of chitin extracted from shrimp shells, 20 mL of high-production biosurfactant bacterial liquid, and 20 mL of high-efficiency petroleum-degrading bacterial liquid were used to prepare 1.0 g of bacterial powder. Simulate 100mL of seawater polluted by oil, the initial concentration of oil is 1700mg / L, and the salinity of seawater is 3%. Sprinkle 1.0g of bacteria powder into a conical flask filled with 100mL of simulated oil-polluted seawater, then put the conical flask into a shaker, the temperature of the shaker is controlled at 25°C, and the time for the bacteria powder to treat oil-polluted seawater is 2 days . Small test results: the degradation rate of total petroleum hydrocarbons in simulated seawater is about 88%, and the concentration of remaining total petroleum hydrocarbons in seawater is 200mg / L, which is higher than the secondary standard in the "Discharge Standards for Oily Wastewater of Offshore Oil Development Industry".
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