Method for enhancing petroleum pollution removing effect of seawater

A CNPC and seawater technology, which is applied in the field of improving the removal effect of oil pollution in seawater, can solve problems such as water environment and aquatic organism damage, and achieve the effects of reducing treatment costs, safety in production process, and improving removal rate.

Inactive Publication Date: 2012-05-02
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the increasingly serious marine oil pollution and the near-destructive damage caused by chemical methods to the water environment and aquatic organisms, the purpose of the present invention is to provide a safe, convenient, low-consumption, and non-secondary to the water environment. Method for improving the effect of oil pollution removal in seawater

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In this example, high biosurfactant-producing strains and high-efficiency oil-degrading strains were respectively inserted into liquid medium for fermentation and activation. Take 1 mL of the fermentation broth of high-yielding biosurfactant bacteria and high-efficiency petroleum-degrading bacteria, respectively, and insert them into 90 mL of liquid medium, and carry out mixed culture in a shaker for 48 hours. Then add 1 g of chitin and 2 g of mannitol extracted from shrimp shells, and continue culturing for 24 hours. After the mixed bacterial solution was frozen for 3 days and dried for 3 days, 2.0 g of bacterial powder was made.

Embodiment 2

[0029] In this example, 0.55 g of chitin extracted from shrimp shells, 20 mL of high-production biosurfactant bacterial liquid, and 20 mL of high-efficiency petroleum-degrading bacterial liquid were used to prepare 1.0 g of bacterial powder. Simulate 100mL of seawater polluted by oil, the initial concentration of oil is 1700mg / L, and the salinity of seawater is 3%. Sprinkle 1.0g of bacterial powder into a conical flask filled with 100mL of simulated oil-polluted seawater, then put the conical flask into a shaker, the temperature of the shaker is controlled at 25°C, and the time for bacterial powder to treat oil-polluted seawater is 1d . Small test results: the degradation rate of total petroleum hydrocarbons in simulated seawater is about 50%, and the concentration of remaining total petroleum hydrocarbons in seawater is 845mg / L, which is much higher than the second grade in the "Discharge Standard for Oily Wastewater from Offshore Oil Development Industry" standard.

Embodiment 3

[0031] In this example, 0.55 g of chitin extracted from shrimp shells, 20 mL of high-production biosurfactant bacterial liquid, and 20 mL of high-efficiency petroleum-degrading bacterial liquid were used to prepare 1.0 g of bacterial powder. Simulate 100mL of seawater polluted by oil, the initial concentration of oil is 1700mg / L, and the salinity of seawater is 3%. Sprinkle 1.0g of bacteria powder into a conical flask filled with 100mL of simulated oil-polluted seawater, then put the conical flask into a shaker, the temperature of the shaker is controlled at 25°C, and the time for the bacteria powder to treat oil-polluted seawater is 2 days . Small test results: the degradation rate of total petroleum hydrocarbons in simulated seawater is about 88%, and the concentration of remaining total petroleum hydrocarbons in seawater is 200mg / L, which is higher than the secondary standard in the "Discharge Standards for Oily Wastewater of Offshore Oil Development Industry".

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Abstract

The invention belongs to the technical field of environmental pollution, in particular to a method for enhancing the petroleum pollution removing effect of seawater. The method comprises the following steps of: culturing high-yield biological surfactant bacteria and efficient petroleum degrading bacteria cultured and screened in a biological trickling filter in a mixed way by taking chitosan as a carrier, and performing freeze drying to obtain a microbial agent (bacterial powder); and treating petroleum pollution simulation seawater with the bacterial powder, wherein three days later, the initial concentration is 1,700 mg/L, the salinity is 3 percent, the removing rate of total petroleum hydrocarbon in the simulation seawater is over 95 percent, and the total petroleum hydrocarbon concentration in the seawater is lower than the primary standard in the Effluent Standards for Oil-Bearing Waste Water from Offshore Petroleum Development Industry. In the method, chitosan is taken as a carrier, so that microorganisms better float on the sea level and are tightly contacted with pollutants on the surface of seawater, and the petroleum removing rate is increased; moreover, the treatment cost of oil stains is reduced greatly; and the production process of the bacterial powder is safe, the application method is simple, and secondary environmental pollution is prevented.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for improving the effect of removing oil pollution in seawater. Background technique [0002] With the continuous and rapid development of my country's economy, the dependence on oil has been increasing. Sea transportation is the main channel for my country's oil imports. At the same time, factors such as offshore oil exploitation have caused more and more serious oil pollution in the ocean. The environmental protection of the ocean is becoming the focus of attention of all countries in the world. [0003] The biodegradation of petroleum pollutants refers to a controlled or spontaneous process that uses biological metabolic activities to catalyze the degradation of oil pollutants, thereby removing or eliminating environmental petroleum pollution. Compared with physical and chemical methods, the biological method has great advantages, and has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N11/10C02F103/08C12R1/44C12R1/07
Inventor 李洪静余焱蒋晓璇张志坚董文博
Owner FUDAN UNIV
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