Production method for microbial germ powder

Abstract

The invention discloses a production method for microbial germ powder, which has the technical steps that: the heat treatment is carried out on proper production seeds after primary seed culture at the treatment temperature being 60 to 90 DEG C for 5 to 60 minutes, the heat treatment is carried out after secondary seed culture, the heat treatment and the cold treatment are carried out after tertiary seed culture, then, the materials are inoculated into a liquid culture medium and is cultured for 16 to 36 hours in a fermentation tank, a liquid culture medium and/or alkali are/is supplemented into the fermentation tank in real time according to the real-time data dynamically detected in the fermentation culture process, germ sledge is obtained after fermentation liquid is separated, the germ sledge is uniformly mixed with absorbing agents, and germ powder is obtained after the crushing and the sieving. The method has the advantages that the viable count of the fermentation liquid is greatly improved, the fermentation factor and the yield are high, the energy consumption is low, the cost is low, the product quality standard is improved, microbes realize the synchronously growth in the fermentation culture process of the seeds after the heat treatment and cold treatment, the viable count of the obtained germ powder is high, the spore mature speed is high, the spore conversion rate is high, the fermentation level is high, and the viable germ yield and the survival rate are high.

Description

technical field [0001] The invention relates to a microorganism cultivation method, in particular to a treatment method suitable for fermentation cultivation of bacillus and drying of bacteria sludge. [0002] Background technique [0003] In the field of microorganisms, it often involves the cultivation and proliferation of strains, so that the strains can be multiplied in a suitable medium under certain temperature conditions to expand the number, so as to meet the requirements of the strains in the fields of human medicine, veterinary medicine, feed, breeding, etc. use needs. Especially for Bacillus, such as Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Clostridium butyricum, etc., it is necessary to ferment and proliferate in the fermenter to significantly increase the number of viable bacteria. [0004] According to the microbial life cycle theory, the microbial growth process can be divided into: adaptation period, logarithmic growth period, equilibriu...

AI Technical Summary

Problems solved by technology

[0008] The present invention mainly adopts the traditional batch low-density fermentation method for microbial fermentation in the prior art, and adopts freeze-drying to the bacteria sludge obtained by centrifugal separation of the fermentation broth, the concentration of bacteria in the fermentation broth is low, and the number of viable bacteria in the bacteria powder is not high. Low fermentation coefficient, low output, high energy consumption, high cost and other deficiencies, a production method of microbial powder is provided; this method can greatly increase the number of viable bacteria in the fermentation broth of Bacillus (such as Clostridium butyricum) and greatly increase the fermentation coefficient and product quality, greatly shorten the production cycle, greatly reduce energy consumption and production costs, and reduce environmental damage