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Feed-use high-activity lactobacillus solid preparation and preparation method thereof

A lactic acid bacteria, high activity technology, applied in the field of feed high activity lactic acid bacteria solid preparation and its preparation, microbial feed additives, can solve the problems of poor repeatability and stability, reduced biological activity, single preparation formula, etc., to achieve stable morphological structure , The effect of reducing the ratio of feed to meat and the number of intestinal villi

Active Publication Date: 2013-11-20
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easy to be inactivated during transportation, use and storage, thereby reducing the biological activity, resulting in poor repeatability and stability of the effect of the preparation
[0005] (2) Stability issues during feed processing
[0007] (4) The preparation formula is single and the versatility is not strong, thus limiting the application of the same process or method in lactic acid bacteria preparations prepared by non-specified strains

Method used

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  • Feed-use high-activity lactobacillus solid preparation and preparation method thereof
  • Feed-use high-activity lactobacillus solid preparation and preparation method thereof
  • Feed-use high-activity lactobacillus solid preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Preparation of plant extracts

[0042] Dry 1 to 10 parts of eucommia leaves, tangerine peel and licorice at 40°C to 55°C until the moisture content is ≤10%, crush them to 60 to 80 mesh, extract 10 to 50 parts of absolute ethanol for 1 to 3 hours, and filter. Rotary evaporation to obtain the extract (density 0.7 ~ 1.5g / cm 3 ), by adding 10 to 200 parts of water.

[0043] (2) Preparation of MRS medium and optimal medium

[0044] The preparation of MRS medium: take peptone 1% (W / V), glucose 2% (W / V), beef extract 1% (W / V), anhydrous sodium acetate 0.5% (W / V), yeast extract Ointment 0.5% (W / V), Tween 800.1ml, Dipotassium hydrogen phosphate 0.2% (W / V), Diammonium hydrogen citrate 0.2% (W / V), Magnesium sulfate heptahydrate 0.058% (W / V) , manganese sulfate monohydrate 0.025% (W / V), water 100ml, adjust the pH to 7.0±0.2; use moist heat sterilization, the temperature is 115 ℃, and the sterilization time is 30min.

[0045] The preparation of the preferred medium: 1-4% (W / ...

Embodiment 2

[0074] (1) Preparation of LB medium and preparation of optimal medium

[0075] Preparation of LB medium: take tryptone 1% (W / V), yeast extract 0.5% (W / V), sodium chloride 1% (W / V), water 100ml, pH7.0±0.2; Sterilization, 121°C, 20min.

[0076] The preparation of the preferred medium: 1-4% (W / V) of sucrose, 1-3% (W / V) of corn steep liquor, 0.4-2% (W / V) of yeast extract, 0.06-0.1% (W / V) of magnesium chloride ), manganese sulfate 0.04-0.2% (W / V), calcium carbonate 0.1-0.4% (W / V), sodium chloride 0.2-0.6% (W / V), water 100ml, pH7.0±0.2; Sterilize, the temperature is 115°C, and the sterilization time is 30min.

[0077] (2) Preparation of bacteria slime

[0078] a. Enterococcus faecalis (ATCC19433) was selected, and the strain was cultured in LB medium at 35°C for 8 hours to obtain a first-grade seed liquid;

[0079] b. Put the primary seed liquid into the seed tank according to the inoculum amount of 2% to expand the cultivation, and cultivate it in LB medium for 6 hours under mi...

Embodiment 3

[0109] (1) Prepare culture medium

[0110] Prepare LB medium: take tryptone 1% (W / V), yeast extract 0.5% (W / V), sodium chloride 1% (W / V), water 100ml, pH7.0±0.2; Sterilization, 121°C, 20min.

[0111] Prepare optimal medium: sucrose 1-4% (W / V), corn steep liquor 1-3% (W / V), yeast extract 0.4-2% (W / V), magnesium chloride 0.06-0.1% (W / V) , manganese sulfate 0.04-0.2% (W / V), calcium carbonate 0.1-0.4% (W / V), sodium chloride 0.2-0.6% (W / V), water 100ml, pH7.0±0.2; sterilization method : Moist heat sterilization, 115°C, 30min.

[0112] (2) Preparation of bacteria slime

[0113] a. Select Lactobacillus lactis (ATCC12315), Bacillus bulgaricus (ATCC11842) and Enterococcus lactis (ATCC19435), and culture the strains in LB medium under microaerobic conditions (ie 0.3mg / L dissolved oxygen) at 35°C 8h, obtain the primary seed liquid of three kinds of bacteria;

[0114] b. Put the first-level seed liquid of the three bacteria into the seed tank according to the inoculum amount of 2% to...

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Abstract

The invention discloses a feed-use high-activity lactobacillus solid preparation and a preparation method thereof. The feed-use high-activity lactobacillus solid preparation comprises growing beneficial lactobacillus bacterium mud, a protection formula and a basic formula. The invention also provides the preparation method of the feed-use high-activity lactobacillus solid preparation, lactobacillus can be protected through synergistic effects of components in the protection formula and the basic formula, and the living bacterium survival rate in the whole process can be improved. The lactobacillus in the prepared feed-use high-activity lactobacillus solid preparation has improved high temperature and high humidity resistances, has a high survival rate in a simulated intestinal fluid, and a substantial improved animal application effect.

Description

technical field [0001] The invention relates to a microbial feed additive, in particular to a solid preparation of highly active lactic acid bacteria for feeding and a preparation method thereof, belonging to the field of microbial preparations. Background technique [0002] As one of the substitutes of antibiotics, probiotics have been widely used in the field of feed additives. In animals, microorganisms produce energy from undigested chyme, stimulate the intestinal immune system, or provide essential vitamins to regulate the intestinal microbiome and its metabolic activity. Although probiotic preparations have gradually developed into the most promising alternative to antibiotics, probiotics, especially lactic acid bacteria with intestinal adhesion, are easily inactivated during production, processing, storage and use, and are harmful to gastric acid, bile The resistance to salt etc. is poor, therefore, the research and development of the probiotic preparation technology...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16
Inventor 韩伟张晓琳王大为汪洋
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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