Low-carbon carotenoid production method

A carotene and biological demulsifier technology is applied in the field of low-carbon production of carotenoids to achieve the effects of reducing the loss of active substances, improving the proliferation ability, the survival rate of viable bacteria, and improving the extraction rate

Inactive Publication Date: 2016-06-08
邵素英
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem solved by the present invention is to overcome the defects of the existing fermentation preparation of carotenoids, and use the leftovers of canned yellow peaches as the main raw material to obtain fruit juice with high carotenoid content through microwave solidification, freezing and crushing, and biological enzymatic hydrolysis , after adjusting the components to obtain the fermentation medium, inoculate the marine red yeast with high carotenoid production into it for staged inoculation and variable temperature fermentation, so as to maximize the proliferation of marine red yeast, and the fermentation liquid is homogenized by ultra-high pressure, vacuum centrifuged, and demulsified After separation, the emulsion is obtained, and the emulsion is extracted, separated and purified to obtain the finished carotenoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A method for producing carotenoids by fermenting yellow peach skin, comprising the steps of:

[0044] 1) Juice preparation: first put the yellow peach skin into a microwave dryer at a power of 4kW, a material layer thickness of 3cm, and dry at 110°C for 3min, then soak it in a sericin peptide solution with a mass percentage of 12% for 12min, and dry it at -20 After freezing at ℃ for 30 minutes, crush immediately. The thickness of the frozen material layer is 4 cm, and the particle size of the crushed product is 0.4 mm. Then, water twice the amount of the crushed product is added, and the pH value is adjusted to 4.5 with lactic acid. Time 400μs, pulse frequency 250Hz under the conditions of high-voltage pulse electric field treatment for 25min; then microwave irradiation extraction at room temperature under the condition of power 200W for 18min, and at the same time conduct ultrasonic-assisted extraction under the condition of power 250W and frequency 35KHz; add 4% of the...

Embodiment 2

[0058] A method for producing carotenoids by fermenting defective yellow peach pulp, comprising the steps of:

[0059] 1) Juice preparation: first slice the defective yellow peach pulp, put it into a microwave dryer with a power of 3kW, a material layer thickness of 2cm, and dry at 105°C for 2 minutes, and then soak it in a sericin peptide solution with a mass percentage of 10% for 10 minutes , After freezing at -18°C for 20 minutes, crush immediately. The thickness of the frozen material layer is 3 cm, and the particle size of the crushed product is 0.3 mm. Then add water that is 1 times the amount of the crushed product, and adjust the pH value to 3.5 with lactic acid. / cm, pulse time 300μs, pulse frequency 200Hz under the conditions of high-voltage pulse electric field treatment for 20min; then microwave irradiation extraction at room temperature under the condition of power 150W for 15min, and ultrasonic-assisted extraction under the condition of power 200W and frequency 30...

Embodiment 3

[0073] A method for low-carbon production of carotenoids, comprising the steps of:

[0074] 1) Juice preparation: First, slice the residual yellow peach pulp into slices, then evenly mix it with the yellow peach skin at a mass ratio of 3:1, put it into a microwave dryer at a power of 5kW, a material layer thickness of 4cm, and dry at 115°C for 4min, then Soak in sericin peptide solution with a mass percentage of 14% for 15 minutes, freeze at -22°C for 40 minutes, and then crush immediately. The thickness of the frozen material layer is 5 cm, and the particle size of the crushed product is 0.5 mm. Lactic acid adjusts the pH value to 5.5, and conducts high-voltage pulse electric field treatment at room temperature under the conditions of electric field strength 35kV / cm, pulse time 500μs, and pulse frequency 300Hz for 30min; Under the conditions of power 300W and frequency 40KHz, ultrasonic-assisted extraction is carried out; mixed enzymes with 5% of the mass of the extract are a...

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Abstract

The invention discloses a low-carbon carotenoid production method. Trimmings of canned yellow peaches are taken as a main raw material and subjected to microwave color fixing, freezing, breaking and biological enzymolysis, juice with higher carotenoid content is obtained, a fermentation medium is obtained after component adjustment, Rhodotorula benthica with high carotenoid yield is inoculated to the fermentation medium for graded inoculation and temperature-shift fermentation, the Rhodotorula benthica is enabled to be proliferated to the greatest extent, fermentation broth is subjected to ultrahigh-pressure homogenization, vacuum centrifugation, demulsification and separation, an emulsion is obtained, and the finished product carotenoid is obtained after extraction, separation and purification of the emulsion, wherein the cell biomass of the fermentation broth is 38-52 g / L, and the yield of the carotenoid is 47-61 mg / L. The production method is simple, short in cycle, high in efficiency, energy-saving and environment-friendly, adopts low-temperature and bio-processing technologies in the whole process, reduces the production cost, eliminates hidden danger of environmental pollution, lays a firm foundation for sustainable development in the technical field of deep processing of the canned yellow peaches and has positive economic and social development significance.

Description

technical field [0001] The invention relates to the preparation of carotenoids, in particular to a low-carbon method for producing carotenoids. Background technique [0002] Carotenoids are a general term for a group of fat-soluble pigments with antioxidant properties. As a class of important biologically active substances in humans and animals, carotenoids have important functions such as enhancing the body's immunity, anti-radiation, anti-tumor, and treating photosensitivity diseases. , It has a wide range of applications in food, cosmetics, medicine and feed industries. At present, carotenoids have been recognized as Class A nutritional pigments by international organizations such as FAO, European Community and WHO, and in recent years, the industrialization development of carotenoids is gradually taking shape. [0003] The industrial production method of carotenoids can be chemical synthesis or plant extraction. The chemical synthesis of carotenoids is not only technic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 邵素英
Owner 邵素英
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