Method for counting plant pathogenic oomycetes zoospores

Abstract

The invention relates to the fields of microorganisms and phytopathology, and particularly discloses a method for counting plant pathogenic oomycetes zoospores. According to the invention, the cameraphase parameter is set, so that the two morphological characteristics of the zoospores, namely blue spores and orange spores, can be clearly observed, thereby realizing the counting of the zoospores.According to the method, the zoospores and the resting spores can be counted simultaneously, so that the related experiments of the oomycetes zoospores are facilitated, and the accuracy and the precision of experimental results are improved. The method disclosed by the invention is simple and reliable, and all microscopes with imaging equipment can be suitable for counting oomycetes zoospores, sothat an evaluation index is provided for evaluating the severity of plant diseases and the pesticide effect of the bactericide.

Description

technical field [0001] The invention relates to the field of microorganisms and plant pathology, in particular to a counting method / statistical method of plant pathogenic oomycete zoospores. Background technique [0002] Phytopathogenic oomycetes infect plants by releasing zoospores with flagella into the soil water, which are attracted by the host root exudates to swim to new host plants, then stop forming restospores, and germinate to produce germ tubes to infect plants. Due to the hydrophilic nature of phytopathogenic oomycetes, zoospores have become an important medium for oomycetes to infect plants. In view of this, the number of oomycete zoospores has become an important indicator for the severity of plant diseases and the evaluation of fungicides. However, zoospores have a strong ability to move. Therefore, how to count the zoospores of oomycetes in water and obtain the ratio of zoospores to restospores is a technical problem to be solved urgently in the research of o...

AI Technical Summary

Problems solved by technology

Yet this invention has the following disadvantages: (1) is only suitable for catching spores or pollen in the air, and cannot be applied to spores in water or soil, and the scope of use is relatively limited

(2) Unable to accurately count spores

Yet, there are following two problems in this invention: (1) higher requirement is arranged for operating time

If the operation time is long, zoospore flagella will fall off and form immobile resting spores, which will cause greater interference to the process of leaf discs attracting zoospores, thus affecting the accuracy of results

(2) This method needs to prepare host plants and prepare leaf discs in advance, the operation process is relatively cumbersome, and the versatility is not high, and the requirements for soybean varieties are relatively strict

At present, many studies have focused on the inhibitory effect of plant tissue extracts or chemical agents on the swimming process of oomycete zoospores, so as to find the means of prevention and control of plant pathogenic oomycetes. However, due to the rapid swimming of oomycete zoospores At present, the number of zoospores of oomycetes under the microscope is estimated, and it is difficult to accurately count the zoospores and restospores after plant tissue extracts or chemical agents treat Phytophthora zoospore suspensions. Ratio, so that the inhibition experiment of chemical substances on the swimming process of oomycete zoospores cannot be accurately quantified

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