Method for rapid separation of phytophthora capsici and special culture medium thereof

Abstract

The invention provides a method for rapid separation of phytophthora capsici and a special culture medium thereof, and belongs to the technical field of phytopathology research. The separation method comprises the steps of sick sample treatment, disinfection, culture medium preparation, and the like. The sick sample is selected from a freshly-picked sick stem, and a sick sample tissue of 9-11 cm is taken with a juncture of the sick part and the healthy part of the sick sample as a center; the tissue is cleaned with tap water, and is dried in the air to remove surface moisture; the dried tissue is uniformly sprayed with 75% alcohol, then soaked with a 1% sodium hypochlorite solution, and cleaned with sterile water; after drying in the air, the disinfected large block of the sick sample is cut into small blocks on an aseptic operating floor; and separation culture is performed with special culture medium. With the method of the invention, the separation rate of phytophthora capsici reaches 81.99% on average, which is increased by 32.6% when compared with the separation rate of a routine (small block disinfection treatment, small block separation) treatment method on average; the separation time is only 31.6 minutes on average; the separation time is shortened by 50.1% on average; and the operations are simple and practical.

Description

technical field [0001] The invention belongs to the technical field of phytopathology, and in particular relates to a quick separation pepper ( Capsicum annuum Linn) Phytophthora ( Phytophthora capsici Leonian) method and its special medium. Background technique [0002] Capsicum blight is the most important fungal disease of capsicum. It can occur throughout the growth cycle of capsicum. In addition to harming the roots, stems, branches, leaves and fruits of capsicum, it also harms tomato, eggplant, cucumber, melon and other crops. Causes a large area of ​​dead plants. The general field dead plant rate is 20% to 30%, and the serious one is over 90%. It can cause devastating losses and occurs in various planting areas. It is especially rampant in high temperature and high humidity areas. The area is seriously harmed, and the production reduction rate is as high as 60%, which seriously affects the economic benefits of farmers and the development of the pepper industry. ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the technical problems of low separation rate and time-consuming and labor-intensive technical problems in the isolation and cultivation process of Phytophthora capsici, and its purpose is to provide an economical, simple, fast, and high separation rate rapid Method for isolating Phytophthora capsici and special culture medium with high efficiency for isolation and cultivation of Phytophthora capsici

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