Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapid separation of phytophthora capsici and special culture medium thereof

A technique for separation and culture of Phytophthora capsicum, applied in the field of phytopathology, can solve the problems of low separation rate, labor and time consuming, etc., and achieve the effects of shortening separation time and improving separation and culture rate

Inactive Publication Date: 2012-03-28
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the technical problems of low separation rate and time-consuming and labor-intensive technical problems in the isolation and cultivation process of Phytophthora capsici, and its purpose is to provide an economical, simple, fast, and high separation rate rapid Method for isolating Phytophthora capsici and special culture medium with high efficiency for isolation and cultivation of Phytophthora capsici

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapid separation of phytophthora capsici and special culture medium thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Collection and numbering of disease samples

[0022] In this embodiment, 10 disease samples were taken, and the sampling place was Chengjiang County, Yuxi City, Yunnan Province, and the variety was local pepper. The numbers are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10; the disease-like materials are all fresh diseased stems, and each disease-like treatment is repeated 3 times, with 4 dishes for each repetition, and 5 dishes for each dish. Block disease sample, a total of 60, that is, the total number of inoculation points is 60.

[0023] (2) Cutting out diseased samples: Take the numbered diseased stems, take the junction of the diseased part and the healthy part of the diseased part as the center line, cut out the diseased samples with a total length of 9-11 cm in the diseased and healthy parts, and make the diseased part in the diseased part The length of the internal tissue and the length of the healthy internal tissue each account for 1 / 2 of the length of the diseased sam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
separationaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for rapid separation of phytophthora capsici and a special culture medium thereof, and belongs to the technical field of phytopathology research. The separation method comprises the steps of sick sample treatment, disinfection, culture medium preparation, and the like. The sick sample is selected from a freshly-picked sick stem, and a sick sample tissue of 9-11 cm is taken with a juncture of the sick part and the healthy part of the sick sample as a center; the tissue is cleaned with tap water, and is dried in the air to remove surface moisture; the dried tissue is uniformly sprayed with 75% alcohol, then soaked with a 1% sodium hypochlorite solution, and cleaned with sterile water; after drying in the air, the disinfected large block of the sick sample is cut into small blocks on an aseptic operating floor; and separation culture is performed with special culture medium. With the method of the invention, the separation rate of phytophthora capsici reaches 81.99% on average, which is increased by 32.6% when compared with the separation rate of a routine (small block disinfection treatment, small block separation) treatment method on average; the separation time is only 31.6 minutes on average; the separation time is shortened by 50.1% on average; and the operations are simple and practical.

Description

technical field [0001] The invention belongs to the technical field of phytopathology, and in particular relates to a quick separation pepper ( Capsicum annuum Linn) Phytophthora ( Phytophthora capsici Leonian) method and its special medium. Background technique [0002] Capsicum blight is the most important fungal disease of capsicum. It can occur throughout the growth cycle of capsicum. In addition to harming the roots, stems, branches, leaves and fruits of capsicum, it also harms tomato, eggplant, cucumber, melon and other crops. Causes a large area of ​​dead plants. The general field dead plant rate is 20% to 30%, and the serious one is over 90%. It can cause devastating losses and occurs in various planting areas. It is especially rampant in high temperature and high humidity areas. The area is seriously harmed, and the production reduction rate is as high as 60%, which seriously affects the economic benefits of farmers and the development of the pepper industry. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12R1/645
Inventor 杨明英曹继芬赵志坚李向东孙道旺
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com