Rapid identification method for resistance of field tobacco alternaria alternata to three types of germicides

Abstract

The invention discloses a rapid identification method for resistance of field tobacco alternaria alternata to three types of germicides, namely dicarboximide type germicide, phenylpyrrole type germicide and aromatic hydrocarbon type germicide, based on a culture-independent and PCR (Polymerase Chain Reaction) amplification technology, belonging to the fields of plant pathology and molecular biology. In the method, tobacco alternaria alternata genome DNA (Deoxyribonucleic Acid) in a tobacco alternaria alternata scab tissue block is extracted and is used as a template, and six groups of amino acid repeating regions, 92 in total, at an end N of bi-component histidine kinase are rapidly amplified by applying a specific primer. Whether the drug resistance of the tobacco alternaria alternata to the dicarboximide type germicide, the phenylpyrrole type germicide and the aromatic hydrocarbon type germicide is generated is determined by comparing gene sequences. The method has the advantages of rapidness, easiness, convenience, accuracy and the like and has a broad application prospect in resistance monitoring of the tobacco alternaria alternata.

Description

technical field

[0001] The invention relates to a method for rapid identification of resistance to three types of fungicides of the field tobacco red spot fungus, terephthalimides, pyrrolebenzenes and aromatic hydrocarbons, based on culture-free and PCR amplification techniques, belonging to plant pathology and molecular field of biology. Background technique

[0002] Alternaria longifera ( Alternaria longipes ) infection caused by field tobacco red spot disease is an important tobacco fungal disease. Dicarboximide, Phenylpyrrole, and Aromatic hydrocarbon fungicides are widely used in the control of many groups of plant pathogenic fungi because of their broad bactericidal spectrum and remarkable control effect. . However, with the prolongation of the use time and the increase of the application amount of these three types of fungicides, many plant pathogenic fungi (including A. longipes ) have serious cross-resistance to the three types of fungicides, which has brought ...