7 results about "Histidine kinase" patented technology

The invention provides a metabolic engineering transformation method of high-yield coenzyme Q10 of rhodobacter sphaeroides and application of transformed engineering bacteria. The method comprises the following steps: knocking out geranyl and geranyl pyrophosphate synthase gene crtE participating in a carotenoid synthesis route; performing overexpression on a farnesyl pyrophosphate synthase gene ispA; a histidine kinase coding gene prrB is mutated, and the mutant is introduced. According to the invention, a combined regulation strategy suitable for targeted regulation is found, the metabolic flux synthesized by the coenzyme Q10 is accurately optimized, and the capability of producing the coenzyme Q10 by the rhodobacter sphaeroides is greatly improved.

The present invention relates to the field of genetic engineering technology, and specifically discloses a histidine kinase mutant, a recombinant microorganism containing the same, and applications thereof. The histidine kinase mutant provided by the present invention, using the amino acid sequence of the wild-type histidine kinase as a reference sequence, contains a mutation in which glutamic acid at position 53 is replaced by aspartic acid, or a mutation in which glutamic acid at position 58 is replaced by another amino acid. Specifically, the mutation contains a substitution of glutamic acid at position 58 by aspartic acid or arginine. Recombinant microorganisms containing the mutants of the present invention have a stronger ability to produce amino acids, especially lysine, and provide a new method for increasing amino acid yield through fermentation.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention relates to histidine kinase PhoG capable of inducing by utilizing the change of phosphate concentration in an external environment and application, a nucleotide sequence of a phoG gene for coding histidine kinase is shown as SEQ ID NO.1, and application of histidine kinase PhoG in regulating and controlling the biosynthesis of a phosphonic acid natural product phospholactam A. When the change range of phosphate concentration is 0.3 mM to 5.1 mM, the histidine kinase PhoG can be used for regulating and controlling the biosynthesis of the phosphonic acid natural product phospholactam A. The histidine kinase PhoG can be used for inducing the change of phosphate concentration in the external environment. The histidine kinase PhoG can be used for regulating and controlling the biological synthesis of the phosphonic acid natural product phospsolactam A. The invention discloses a natural product yield regulation and control strategy based on environmental sensing, provides a novel sensing element for activating the expression of silent gene clusters and improving the yield of phosphonic acid natural products containing phosphonic acid groups / phosphinic acid groups, and has good application prospects and industrial development potential.

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.