33 results about "Histidine kinase" patented technology

Compounds that competitively inhibit binding of CSP to S. mutans histidine kinase are provided. The compounds are preferably a peptide or an antibody, and are preferably a derivative of [SEQ ID NO:2], a fragment of [SEQ ID NO:2] or a derivative of a fragment of [SEQ ID NO:2]. Methods of making these compounds and their use for inhibiting the growth of S. mutans, for inhibiting dental caries, and for improving dental health are also disclosed.

A method of screening a compound for anti-fungal properties comprising the steps of exposing a test compound to a fungal histidine kinase, and determining whether kinase activity is inhibited, wherein inhibition of kinase activity indicates that the compound has anti-fungal properties is disclosed.

The invention discloses a method for improving a nisin induction efficiency in a lactic acid bacteria NICE expression system. According to the method disclosed by the invention, the sensing activity of nisin is enhanced by conducting mutation transformation on receptor histidine kinase NisK of nisin molecules of an inducer. Experiments prove that through nisin induction of a same concentration, the expression amount of reporter protein is obviously increased and the nisin induction efficiency is remarkably enhanced after the NisK mutation transformation; therefore, the method disclosed by the invention has a quite important significance for improving the expression amount of heterologous protein, declining the concentration of the inducer and reducing the complexity of constructing the system by virtue of the NICE system.

The invention discloses a protein for regulating Cronobacter sakazakii's resistance to high stress, its encoding gene and application. The gene has the sequence shown in SEQ ID NO:2. The invention uses genetic engineering technology to knock out the high pressure resistance gene CpxA of Cronobacter sakazakii, so that the expression of histidine kinase in it is significantly reduced, thereby significantly reducing the integrity of the cell membrane of Cronobacter sakazakii, so that the sakazakii The anti-stress performance of Cronobacter sakazakii is greatly weakened, which can not only effectively inhibit the formation of Cronobacter sakazakii biofilm, but also achieve better killing effect of Cronobacter sakazakii under lower pressure. There are broad application prospects in other fields.

The invention discloses a Clostridium modified by a kinase gene with a phosphorylation function and its application; the method is a method for improving the production of butanol through the gene modification of a kinase with a phosphorylation function, specifically knocking out phosphorylation in Clostridium The function obtains the kinase (including histidine kinase, serine kinase, threonine kinase) gene, thereby regulating the downstream gene expression. After knocking out or overexpressing the kinase gene with phosphorylation function in the present invention, the butanol output of the mutant strain has a significant change compared with the wild strain, and some kinases participate in spore formation and cell morphology changes, so that the Clostridium cells maintain a high vitality state, Thereby improving the butanol synthesis capacity of cells. The Clostridium modified by the phosphorylation kinase gene of the present invention has a good application prospect in the production of butanol.

Provided herein are microorganisms engineered with hybrid receptors and genetic circuits. Also provided are hybrid receptors having a CqsS polypeptide and a heterologous histidine kinase domain of a two-component system. Methods for using engineered microorganisms to sense and destroy pathogens (e.g., Vibrio cholerae) are also provided.

The invention relates to a 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound and a synthesis method and application thereof. The compound has a structure formula as below. The 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound serving as an inhibiting agent of YyCG histidine kinase protein for a staphylococcus epidermidis signal transduction system has a novel structure, and the adopted method for preparing the compound is simple and easy to implement. The test results of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) show that the compound has excellent inhibitory action on the formation of a staphylococcal biological film and can effectively kill and wound the bacteria in the staphylococcal biological film.

The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like.

The invention discloses uses of a histidine kinase gene Hisk2301, particularly applications of a histidine kinase gene Hisk2301 in production of polyunsaturated fatty acids at a low temperature. According to the present invention, polyunsaturated fatty acids are produced at a low temperature with the histidine kinase gene Hisk2301, and the contents of the polyunsaturated fatty acids such as LA and ALA are improved by modifying microorganisms through genetic engineering, such that the good application prospects and economic benefits are provided for the industrial production of polyunsaturated fatty acids, and the foundation is established for the large-scale industrial production of polyunsaturated fatty acids.

The present invention provides a hybrid-type histidine kinase gene isolated from indica rice IR64, and being capable of osmosensing and inducible by multiple stresses, and hence, being capable of improving the multiple stress tolerance of the crop plants even in subsequent generations so as to make the plants capable of coping-up with the more than one environmental abiotic stress conditions, and therefore, increasing the economic value of the crop plants while maintaining the yield thereof. The present invention also provides a method of isolation of hybrid-type histidine kinase gene from indica rice IR64, and its functional characteristics and its sequence listing and cloning, at least, with yeast expression vector and the plant expression vector, and clones produced thereby, and method of improving multiple stress tolerance of crop plant and crop plants having improved multiple stress tolerance.

The invention relates to a drug screening model taking histidine kinase AgrC as a target spot, belonging to the field of biological medicines. A construction method of the drug screening model comprises the following steps: (1) extracting and purifying AgrC protein; (2) determining the concentration of AgrC protein; (3) preparing lipidosome by taking DOPC, DPPA and CHOL as raw materials; (4) inserting the extracted and purified AgrC protein into the lipidosome by adopting a surfactant-mediating method, thus forming protein lipidosome; and (5) constructing the drug screening model taking the histidine kinase AgrC as the target spot. The constructed drug screening model is suitable for screening drug monomeric compounds having a strong inhibiting effect for histidine kinase AgrC, and the screened compounds are definite in acting sites; the drug screening model has the advantages of high flux, strong specificity and operation convenience and rapidness when used for screening the drug monomeric compounds.