12 results about "Histidine kinase" patented technology

The invention provides a signal detection method for exploring the influence of a histidine kinase inhibitor on bacterial drug resistance, which comprises the following steps of: respectively acquiring images of a bacterial culture group added with the histidine kinase inhibitor, a culture group added with glucose and bacterial control and a control group; respectively carrying out feature point detection and marking on the culture group image and the control group image by using a feature point detection algorithm; traversing and counting the number of feature points of the culture group image and the control group image, calculating a bacterial growth change rate delta, and generating a corresponding bacterial drug resistance influence curve according to the sampling frequency and the corresponding bacterial growth change rate delta. Signal detection and analysis of the influence of the histidine kinase inhibitor on the bacterial drug resistance can be realized by using image recognition and feature point detection technologies, so that the influence of the inhibitor on the bacterial drug resistance is evaluated. Therefore, the whole steps are simplified, too many test equipment and process steps are not needed, the whole method is realized by depending on an electronic program, the test flow and cost are saved, and the drug resistance detection efficiency is accelerated.

The invention provides a metabolic engineering transformation method of high-yield coenzyme Q10 of rhodobacter sphaeroides and application of transformed engineering bacteria. The method comprises the following steps: knocking out geranyl and geranyl pyrophosphate synthase gene crtE participating in a carotenoid synthesis route; performing overexpression on a farnesyl pyrophosphate synthase gene ispA; a histidine kinase coding gene prrB is mutated, and the mutant is introduced. According to the invention, a combined regulation strategy suitable for targeted regulation is found, the metabolic flux synthesized by the coenzyme Q10 is accurately optimized, and the capability of producing the coenzyme Q10 by the rhodobacter sphaeroides is greatly improved.

To provide methods and means for assessing or responding to a protein-protein interaction and transducing this interaction to the expression of a gene of interest.SOLUTION: The present invention relates to a cell comprising a first nucleic acid sequence encoding a first polypeptide fused to N-terminus of a first variant of a histidine kinase comprising a DHp domain and a CA domain, (wherein the first variant does not comprise a transmembrane domain), a second nucleic acid sequence encoding a second polypeptide fused to the N-terminus of a second variant of the histidine kinase comprising a DHp domain and a CA domain, (wherein the second variant does not comprise a transmembrane domain), and a third nucleic acid sequence encoding a response regulatory protein specifically phophorylatable by the DHp domain of the first or the second variant. The present invention further relates to uses of the cell of the invention.SELECTED DRAWING: Figure 4A

The present invention relates to the field of genetic engineering technology, and specifically discloses a histidine kinase mutant, a recombinant microorganism containing the same, and applications thereof. The histidine kinase mutant provided by the present invention, using the amino acid sequence of the wild-type histidine kinase as a reference sequence, contains a mutation in which glutamic acid at position 53 is replaced by aspartic acid, or a mutation in which glutamic acid at position 58 is replaced by another amino acid. Specifically, the mutation contains a substitution of glutamic acid at position 58 by aspartic acid or arginine. Recombinant microorganisms containing the mutants of the present invention have a stronger ability to produce amino acids, especially lysine, and provide a new method for increasing amino acid yield through fermentation.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention relates to a construction method of engineering bacteria for high-yield undecyl prodigiosin, and belongs to the technical field of biology. According to the invention, 16 isolated histidine kinase coding genes with unknown functions in streptomyces coelicolor are inhibited by using a dCas9 mediated CRISPRi technology, strains with significant phenotypic changes are screened out, an OhkB gene is knocked out by using a CRISPR-Cas9 technology, and it is detected that the yield of undecyl prodigiosin is increased, so that the yield of undecyl prodigiosin is increased. And further determining that the OhkB gene can negatively regulate and control the biosynthesis of undecylprodigiosin and actinopurpurin through a refilling experiment. The invention provides a novel regulatable gene locus for increasing the yield of the undecylprodigiosin and the actinopurpurin, and lays a foundation for further development and utilization of the undecylprodigiosin and the actinopurpurin.

This invention relates to compositions and methods for modifying Cytokinin Receptor Histidine Kinase (HK) genes in plants, optionally to improve yield traits, the HK genes encoding a histidine kinase (HK) polypeptide. The invention further relates to plants having increased improved yield traits produced using the methods and compositions of the invention.

The invention relates to histidine kinase PhoG capable of inducing by utilizing the change of phosphate concentration in an external environment and application, a nucleotide sequence of a phoG gene for coding histidine kinase is shown as SEQ ID NO.1, and application of histidine kinase PhoG in regulating and controlling the biosynthesis of a phosphonic acid natural product phospholactam A. When the change range of phosphate concentration is 0.3 mM to 5.1 mM, the histidine kinase PhoG can be used for regulating and controlling the biosynthesis of the phosphonic acid natural product phospholactam A. The histidine kinase PhoG can be used for inducing the change of phosphate concentration in the external environment. The histidine kinase PhoG can be used for regulating and controlling the biological synthesis of the phosphonic acid natural product phospsolactam A. The invention discloses a natural product yield regulation and control strategy based on environmental sensing, provides a novel sensing element for activating the expression of silent gene clusters and improving the yield of phosphonic acid natural products containing phosphonic acid groups / phosphinic acid groups, and has good application prospects and industrial development potential.

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

The invention discloses streptococcus agalactiae and application of the streptococcus agalactiae in preparation of drugs for treating drug-resistant streptococcus agalactiae infection, the streptococcus agalactiae is streptococcus agalactiae S.aXJM001, the streptococcus agalactiae S.aXJM001 is preserved in the China General Microbiological Culture Collection Center, the preservation date is January 17, 2025, and the biological preservation number is CGMCC NO.33461. The invention further discloses a preparation method of the streptococcus agalactiae. According to the invention, a homologous recombination technology is adopted to construct a CovS and CovR double-gene knockout mutant strain, a histidine kinase inhibitor is combined to block a signal channel, qRT-PCR (quantitative reverse transcription-polymerase chain reaction) is adopted to measure signal molecules and virulence and abundance change of drug-resistant genes, and the influence of CovS and CovR genes on the virulence and drug resistance of streptococcus agalactiae is explored; the molecular regulation mechanism of the CovS / CovR system mediated streptococcus agalactiae virulence and drug resistance is clarified, and theoretical and technical bases are provided for analysis and research of streptococcus agalactiae drug treatment targets, so that generation of drug resistance is reduced, and the CovS / CovR system mediated streptococcus agalactiae virulence and drug resistance molecular regulation method has important significance for guaranteeing quality safety of milk and dairy products.

Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

Provided herein are cell-free systems comprising two-component systems including a histidine kinase and a response regulator. In embodiments, the systems further include synthetic membranes. Also provided are methods for using the systems to detect analytes and synthesize target molecules.