33 results about "Histidine kinase" patented technology

The invention relates to a 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound and a synthesis method and application thereof. The compound has a right structure formula. The 3-carbonyl-6-ethoxycarbonyl-thiazole pyrimidine compound serving as an inhibiting agent of YyCG histidine kinase protein for a staphylococcus epidermidis signal transduction system has a novel structure, and the adopted method for preparing the compound is simple and easy to implement. The test results of the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) show that the compound has excellent inhibitory action on the formation of a staphylococcal biological film and can effectively kill and wound the bacteria in the staphylococcal biological film.

The invention relates to a compound that competitively inhibits binding of CSP to S. mutans histidine kinase. The compound is preferably a peptide or an antibody. The compound is preferably a derivative of [SEQ ID NO:2], a fragment of [SEQ ID NO:2] or a derivative of a fragment of [SEQ ID NO:2].

Compounds that competitively inhibit binding of CSP to S. mutans histidine kinase are provided. The compounds are preferably a peptide or an antibody, and are preferably a derivative of [SEQ ID NO:2], a fragment of [SEQ ID NO:2] or a derivative of a fragment of [SEQ ID NO:2]. Methods of making these compounds and their use for inhibiting the growth of S. mutans, for inhibiting dental caries, and for improving dental health are also disclosed.

The invention discloses uses of a histidine kinase gene Hisk2301, particularly applications of a histidine kinase gene Hisk2301 in production of polyunsaturated fatty acids at a low temperature. According to the present invention, polyunsaturated fatty acids are produced at a low temperature with the histidine kinase gene Hisk2301, and the contents of the polyunsaturated fatty acids such as LA and ALA are improved by modifying microorganisms through genetic engineering, such that the good application prospects and economic benefits are provided for the industrial production of polyunsaturated fatty acids, and the foundation is established for the large-scale industrial production of polyunsaturated fatty acids.

The invention discloses clostridium modified by a kinase gene with a phosphorylation function and application of the clostridium. A method is a method for modifying by the kinase gene with the phosphorylation function to improve the yield of butanol; specifically, the phosphorylation function in the clostridium is knocked out to obtain the kinase (including histidine kinase, serine kinase and threonine kinase) gene, so that downstream gene expression is regulated and controlled. After the kinase gene with the phosphorylation function is knocked out or overexpressed, the yield of the butanol inmutant strains is remarkably changed when being compared with that of wild strains; some kinases participate in spore formation and cell morphological changes, so that clostridium cells keep a high-activity state and the butanol synthesis capability of the cells is improved. The clostridium modified by the kinase gene with the phosphorylation function has a good application prospect in the aspectof production of the butanol.

The invention discloses a method for improving a nisin induction efficiency in a lactic acid bacteria NICE expression system. According to the method disclosed by the invention, the sensing activity of nisin is enhanced by conducting mutation transformation on receptor histidine kinase NisK of nisin molecules of an inducer. Experiments prove that through nisin induction of a same concentration, the expression amount of reporter protein is obviously increased and the nisin induction efficiency is remarkably enhanced after the NisK mutation transformation; therefore, the method disclosed by the invention has a quite important significance for improving the expression amount of heterologous protein, declining the concentration of the inducer and reducing the complexity of constructing the system by virtue of the NICE system.

Provided herein are microorganisms engineered with hybrid receptors and genetic circuits. Also provided are hybrid receptors having a CqsS polypeptide and a heterologous histidine kinase domain of a two-component system. Methods for using engineered microorganisms to sense and destroy pathogens (e.g., Vibrio cholerae) are also provided.

The invention belongs to the field of biotechnology, and relates to a novel application of a compound FDEFA1 in pharmacy, in particular to an application of a compound FDEFA1 in a formula (1) in preparation of a gram-positive coccus inhibitor. The compound FDEFA is used as an inhibitor for inhibiting gram-positive coccus YycG histidine kinase, and can inhibit growth of Gram-positive bacteria and formation of biological membranes; particularly, the compound has an inhibition effect on YycG kinase in an enterococcus faecalis two-component signal transduction system, and is used for inhibiting the formation of floating bacteria and biological membranes of enterococcus faecalis, enterococcus faecium, staphylococcus aureus, streptococcus agalactiae and staphylococcus epidermidis; the compound can be used for preparing a gram-positive coccus inhibitor and a medicine for resisting enterococcus faecalis infection or treating diseases caused by gram-positive bacterium infection.

The invention discloses application of deoxyshikonin (5,8-dihydroxy-2-(4-methyl-3-phenyl)-1,4-naphthalenedione) as a histidine kinases VicK inhibitor in vitro or to preparation of the histidine kinases VicK inhibitor. Deoxyshikonin exerts certain antibacterial action on in-vivo streptococcus pneumonia and clinical penicillin-resistant streptococcus pneumonia; moreover, combined deoxyshikonin and penicillin exert a good antibacterial effect on streptococcus pneumonia and has substantial bacteriostatic action on staphylococcus aureus.

The invention discloses a streptococcus mutans detection method based on a quorum sensing system and application of the streptococcus mutans detection method, and belongs to the technical field of genetic engineering. The detection method is established based on the quorum sensing system; elements of the sensing system comprise a gene PelB-comD compounded by a signal peptide of escherichia coli and a histidine kinase receptor membrane protein gene, an intracellular reaction regulation protein gene comE, and a gene nlmC-sfGFP compounded by a promoter nlmC, regulated and activated by comE, and a green fluorescence signal reporter gene sfGFP. According to the detection method, the signal molecule CSP secreted and expressed by the streptococcus mutans is subjected to specific recognition response through the sensing system, and then the streptococcus mutans is detected. The detection method disclosed by the invention can specifically recognize and detect streptococcus mutans, has the characteristics of convenience in operation and low detection cost, and can be applied to decayed tooth detection so as to evaluate the health condition of teeth.

The invention discloses a protein for regulating pressure stress resistance of cronobacter sakazakii, and a coding gene and application thereof. The gene has a sequence as shown in SEQ ID NO: 2. The high-pressure-resistant gene CpxA of the cronobacter sakazakii is knocked out by utilizing a gene engineering technology, so that the expression of histidine kinase in the high-pressure-resistant gene CpxA is remarkably reduced, the integrity of a cell membrane of the cronobacter sakazakii is further remarkably reduced, the compression resistance of the cronobacter sakazakii is greatly weakened, and the protein not only can effectively inhibit the formation of a cronobacter sakazakii pellicle, but also can achieve a good cronobacter sakazakii killing effect under a low pressure, and has a wide application prospect in the fields of food safety and the like.