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Synthetic hybrid receptor and genetic circuit in bacteria to detect enteric pathogenic microorganisms

a technology of genetic circuit and hybrid receptor, applied in the field of biosynthetic engineering of microorganisms, can solve the problems of low cost, insufficient safety, efficient treatment, etc., and achieve the effects of reducing the spread of bacterial pathogens, rapid response, and saving patients' lives

Active Publication Date: 2020-06-04
TRUSTEES OF BOSTON UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0004]This disclosure provides non-naturally occurring bacteria that detect the CAI-1 molecule made by Vibrio cholerae. The disclosure also describes coupling Vibrio cholerae detection with transcription modulation, enabling a biological response to pathogen detection, including expression of modules that kill and / or inhibit Vibrio cholerae infection or provide a colorimetric signal that the pathogen has been detected. This enables a rapid response to Vibrio cholerae infection in the human intestine, via the hybrid receptor, allowing clinicians to save the patient's life and / or reduce dissemination of the bacterial pathogen.

Problems solved by technology

A major obstacle to controlling cholera infection is the paucity of safe, efficient, and low cost treatments.

Method used

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  • Synthetic hybrid receptor and genetic circuit in bacteria to detect enteric pathogenic microorganisms
  • Synthetic hybrid receptor and genetic circuit in bacteria to detect enteric pathogenic microorganisms
  • Synthetic hybrid receptor and genetic circuit in bacteria to detect enteric pathogenic microorganisms

Examples

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example 1

ng of Lactococcus lactis to Detect and Kill Vibrio cholerae

[0080]The purpose of this technology is to engineer the two-component system of the probiotic bacteria Lactococcus lactis to detect and kill the cholera pathogen Vibrio cholerae. L. lactis is a member of the lactic-acid bacteria family and labeled “generally recognized as safe (GRAS)” by the FDA. Because it can reside in the human intestine with no harm to the body, L. lactis can be an ideal candidate for intestinal pathogen sensing and killing. Cholera is an infectious disease that can cause severe diarrhea. There are three to five million cholera cases every year, resulting in I 00,000-120,000 deaths, mostly in developing countries[l]. The engineered L. lactis will promise a safe, efficient and low-cost treatment for the serious infectious disease cholera.

[0081]A hybrid cell surface receptor that allows L. lactis to detect the Vibrio cholerae quorum-sensing molecule CAI-1 has been developed[2]. To enable CAI-1 detection, ...

example 2

ng of Lactococcus lactis to Detect Vibrio cholerae Using a Colorimetric Based Assay

[0092]The present example describes an engineered Lactococcus lactis (L. lactis) microorganism that can be used to detect (e.g., in vitro) a V. choerae microorganism in a colorimetric assay. L. lactis was engineered to produce ß-lactamase in response to detecting CAI-1 secreted by V. cholerae, which turns a yellow substrate (nitrocefin) red when V. cholerae is detected. β-lactamase is a robust enzyme with a high catalytic efficacy and a small size that makes it easy to diffuse. Wild-type (w.t.) L. lactis were transformed with pHTR plasmid, a schematic of which is shown in FIG. 9. The pHTR plasmid is a derivation of the pHCR2 plasmid, with the mCherry gene replaced by β-lactamase gene. L. lactis transformed with pHTR express ß-lactamase in response to detecting CAI-1 secreted by V. cholerae. The pHTR construct has β-lactamase repressed by TetR, where anhydrotetracycline (ATc) is an inducer that can be ...

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Abstract

Provided herein are microorganisms engineered with hybrid receptors and genetic circuits. Also provided are hybrid receptors having a CqsS polypeptide and a heterologous histidine kinase domain of a two-component system. Methods for using engineered microorganisms to sense and destroy pathogens (e.g., Vibrio cholerae) are also provided.

Description

RELATED APPLICATIONS[0001]This application claims the benefit under 35 U.S.C. § 119(e) of U.S. provisional application No. 62 / 172,971, filed Jun. 9, 2015, which is incorporated by reference herein in its entirety.FIELD OF INVENTION[0002]The present disclosure relates, in some aspects, to the field of biosynthetic engineering of microbes that can detect and / or kill a pathogen, such as Vibrio cholerae. BACKGROUND OF INVENTION[0003]In 2013, 47 countries reported a total of 129,064 cases of cholera including 2,102 deaths, giving a case-fatality rate (CFR) of 1.63%. Cholera represents an estimated burden of 1.4 to 4.3 million cases, and 28,000 to 142,000 deaths per year worldwide (World Health Organization: Weekly epidemiological record, No. 31, 1 Aug. 2014). A major obstacle to controlling cholera infection is the paucity of safe, efficient, and low cost treatments. Thus, there is a need for additional strategies to combat microbial infections, such as Vibrio cholerae. SUMMARY OF INVENT...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/569C07K14/28
CPCG01N33/56911C12N15/625C07K14/28A61K35/744C07K14/195C12N9/12G01N2333/28C12N15/62C12Y305/02006Y02A50/30G01N2800/26
Inventor MAO, NINGCAMERON, DOUGLAS EWENCOLLINS, JAMES
Owner TRUSTEES OF BOSTON UNIV
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