20 results about "Vibrio cholera" patented technology

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The invention relates to the field of biological detection, in particular to a primer probe set, a detection reagent and application of the primer probe set and the detection reagent in detection of virulent pathogenic bacteria. The invention provides a primer probe group. The primer probe group comprises a primer probe group used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae, salmonella typhimurium and staphylococcus aureus, wherein the primer probe group is used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae and salmonella typhimurium. The invention establishes a primer probe combination for rapidly and sensitively detecting various virulent pathogenic bacteria and a kit thereof, aiming at eight virulent pathogenic bacteria such as bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum, vibrio cholerae, brucella, salmonella typhimurium and staphylococcus aureus. The kit is stored in a freeze-dried state, adaptive sample lysate can directly expand different types of samples, and the kit has the advantages of being good in specificity, high in sensitivity, short in detection time, good in accuracy, good in stability after redissolution and good in compatibility.

The invention discloses bacillus velezensis Jcl-10 and application of the bacillus velezensis Jcl-10 in prevention and treatment of vibrio cholerae. The strain Jcl-10 is preserved in the China Center for Type Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC N: M 2024212. The bacillus velezensis Jcl-10 disclosed by the invention is identified as a gram positive bacterium after being subjected to gram staining. The bacterial strain can produce antibacterial peptide substances, antibacterial protein substances, polyketide synthetase substances and the like with bacteriostatic action, and can be well applied to prevention and treatment of aquatic diseases caused by vibrio cholerae. The Jcl-10 is added into the aquatic feed, so that the morbidity of aquaculture animals can be effectively reduced, and the economic yield of the aquaculture animals can be increased. In conclusion, the bacillus velezensis Jcl-10 disclosed by the invention has outstanding performance in the prevention and treatment of vibrio cholerae, and provides a powerful guarantee for continuous and healthy culture of aquatic products.

Disclosed are a chemical synthesis method for Vibrio cholerae serotype O100 O-antigen oligosaccharide, and a use, belonging to the technical field of chemistry. The present invention uses three monosaccharide building blocks and five kinds of carboxylic acid derivative, and under the effects of solvent, temperature and neighboring group participation, through orthogonal protection, selective assembly and amide coupling, five Vibrio cholerae serotype O100 O-antigen oligosaccharide fragments are synthesized. Using the synthesized oligosaccharide fragments, combined with NMR analysis and carbohydrate chip technology, the absolute configuration and immunological function of the 3,5-dihydroxyhexanoyl group in the O-antigen trisaccharide are clarified, providing a theoretical basis for further structure-activity research and minimal antigen epitope screening. The present invention has promising applications in the synthesis of Vibrio cholerae glycoconjugate vaccines and the development of new drugs.

The present application relates to a kind of pseudomonas aeruginosa engineering bacteria DUEC construction method and application.The DUEC engineering strain construction method of the present application is: response to kinase coding gene retS and sheath protein coding gene tssB2, tssB3 Knockout, H1-T6SS secretory effector protein Tse1-Tse4, Tse6-Tse8 catalytic site mutation, Tse5 Coding gene knockout.DUEC engineering strain can not only constitutively express and assemble H1-T6SS, but also can respond to adjacent sister cell source T6SS attack, assemble H1-T6SS at the position of being attacked.The present application constructs a series of effector protein combination inactivation mutant by gene knockout or catalytic site mutation method.Microscope observation and statistical analysis results show that no single effector protein is necessary for T6SS attack and assembly.By bacterial competition experiment, it is verified that all effector protein inactivation mutant 8eff c Lose T6SS + Vibrio cholerae and T6SS + Aeromonas dhaka's bactericidal activity, but 8eff c Can respond to T6SS physical attack, Tse6 N -Cre is specifically delivered to T6SS + In Vibrio cholerae.

The application discloses a recombinant bacillus subtilis of surface display of a fusion protein of Edwardsiella tarda outer membrane protein OmpA and Vibrio cholerae enterotoxin CTB, a construction method and application. A homologous double cross recombinant of coding genes of OmpA and CTB is carried out into a genome of the bacillus subtilis 168 by taking the spore coat protein CotY as an anchor point, so that the recombinant bacillus subtilis capable of stably inheriting in the bacillus subtilis and displaying the Edwardsiella tarda outer membrane protein OmpA and the Vibrio cholerae enterotoxin CTB on a spore surface is obtained. After fish and mammals orally immunize the bacillus subtilis, the survival time and survival rate of the fish and mammals after infection of the Edwardsiella tarda are obviously improved. The recombinant bacillus subtilis is used as an oral vaccine type micro-ecological immunization preparation for preventing and treating the Edwardsiella tarda infection of the fish and mammals, so that the immunization procedure is obviously simplified, the immunization stress is reduced, the labor cost is saved, the effective concentration in the intestinal tract is ensured, and the specific immune response of the body is induced.

The present disclosure discloses a specific saccharide fragment for development of Vibrio cholerae vaccines, and belongs to the field of medicine. In the present disclosure, a saccharide fragment related to a trisaccharide of V. cholerae O100 serotype O-antigen is chemically synthesized. Combined with a glycan microarray technology, the structure-activity relationship between different saccharide fragments and antigenicity thereof is evaluated at a molecular level. Glycan microarray screening indicates that 3-hydroxybutyryl is an essential structural feature of the O-antigen. A non-reducing end disaccharide carrying 3-hydroxybutyryl is a potential minimal antigenic epitope, and the disaccharide has strong binding capacity to antibodies and a simple structure, and may serves as a specific saccharide fragment for vaccine development. A glycoconjugate vaccine designed based on the specific saccharide fragment may solve the challenges of difficulty in culturing pathogenic bacteria and heterogeneity of saccharide antigens in naturally extracted polysaccharide vaccines. The present disclosure has bright application prospects in the development of glycoconjugate V. cholerae vaccine, infection detection, and new drug development.

The utility model discloses a telescopic water sampler for sampling vibrio cholerae in a large water body, which is characterized in that a sampler for placing samples is arranged, a hook is mounted on the upper surface of the sampler, and a sealing door is rotationally connected to the front side of the outer surface of the sampler; comprising a built-in bin installed on the inner surface of the collector, an unlocking groove is formed in the front side of the outer surface of the built-in bin, a water suction pipe is connected between the built-in bin and the collector in a nested mode, and meanwhile a sample tank is connected to the inner surface of the built-in bin in a nested mode. A telescopic assembly for mounting the collector is arranged, a water suction pipe and a sample tank are nested and assembled in the collector, the stability of the sample tank is controlled through cooperation of a clamping piece and a reset spring arranged on the sample pipe, and a telescopic pipe in the telescopic assembly is matched with a water inlet pipe, so that water sampling stretching is adjusted, and stretching adjustment of the water sampler is controlled; the clamping connection of the external bin is matched, so that the influence of impurities on sample collection is avoided, and the stability of vibrio cholerae collection is improved.

This invention discloses a Bacillus tergenta Bte2005, with accession number M 2026736. This invention relates to Bacillus terrestris Bte2005, isolated from the hatching system of healthy Litopenaeus vannamei eggs. This strain and its metabolites exhibit strong antagonistic effects against more than ten pathogenic bacteria of marine aquaculture animals, including Vibrio harveyi, Vibrio Erwini, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio anguillarum, Vibrio cholerae, Vibrio shewani, Vibrio rotiferus, Pseudomonas pseudoalteromonas, and luminescent bacteria of mermaids. It demonstrates strong antibacterial activity and a broad antibacterial spectrum. Bacillus terrestris Bte2005 metabolites are abundant and stable. Its ammonium sulfate precipitate extract, hydrochloric acid precipitate methanol extract, ethyl acetate extract, and chloroform extract all exhibit broad-spectrum antibacterial activity. The functional substances that exert its broad-spectrum antagonistic antibacterial effect are complex and diverse, and it does not exert a targeted selection effect on bacteria in the environment, nor does it easily induce tolerance in pathogens. Therefore, it possesses good application safety and ecological safety properties.

The invention relates to the technical field of microorganisms, in particular to a bacteriophage compound preparation for vibrio harveyi and application. The compound preparation comprises a vibrio harveyi bacteriophage RDP-VB25018 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 46671 and a vibrio harveyi bacteriophage RDP-VB25018R with the preservation number of CGMCC No. 46672. The compound preparation can effectively overcome the problem that bacteria easily generate resistance in bacteriophage treatment, has a high-efficiency synergistic lysis effect on original strains and resistant strains of Vibrio harveyi, has a lysis spectrum width of 84%, is far superior to that of a single bacteriophage, and has a good application prospect. And one or more of vibrio orientalis, vibrio harveyi, vibrio cholerae, vibrio canbainii, vibrio Ewana, vibrio alginolyticus and vibrio parahaemolyticus can be cracked. Animal experiments show that the compound preparation can obviously reduce the death rate of infected prawns from 85% or above to about 11%, and pathogenic bacteria in tissues are thoroughly removed. The invention provides an efficient and safe solution for green prevention and control of aquatic vibriosis.

The invention belongs to the field of biological detection, and provides a portable microbiological detection device which comprises a sample processing module (10) used for collecting and processing samples, and a quantum dot fluorescence detection module (20) is arranged on the sample processing module (10). In the aspect of battlefield environment adaptability, the weight lt of the detection device is detected; handheld operation is supported, a built-in battery is not needed, and passive detection is achieved; the shell is made of an explosion-proof composite material and is resistant to high temperature (-20 DEG C to 60 DEG C) and impact (IP67 protection grade); the quantum dot fluorescent probe is combined with a magnetic separation technology, the detection limit can be as low as 1 CFU / mL, and multi-target simultaneous detection (such as bacillus anthracis and vibrio cholerae) is realized through quantum dot probe ligand design.

The present invention relates to a specific sugar fragment for the research and development of Vibrio cholerae vaccines. By means of a chemical synthesis method and sugar fragments related to the Vibrio cholerae O100 serotype O antigen trisaccharide, combined with sugar chip technology, the structure-activity relationship between different sugar fragments and the antigenicity thereof is evaluated at the molecular level. Sugar chip screening shows that 3-hydroxybutyryl is a key structural feature of the O antigen. The non-reducing terminal disaccharide carrying 3-hydroxybutyryl is the smallest potential antigen epitope. The disaccharide has a strong ability to bind to an antibody, has a simple structure, and can be used as a specific sugar fragment for the research and development of vaccines. Sugar-conjugated vaccines designed on the basis of the specific sugar fragment can solve challenges such as difficult culture of pathogenic bacteria and inhomogeneous sugar antigens in naturally extracted polysaccharide vaccines. The present invention has good application prospects in the research and development of Vibrio cholerae sugar-conjugated vaccines, the detection of infections, the development of novel drugs, etc.

The invention relates to a Vibrio cholerae MakA (motility associated killing factor A) protein for use in therapy, in particular in the treatment or alleviation of cancer such as colorectal cancer. The invention further relates to pharmaceutical compositions comprising a Vibrio cholerae MakA protein as an active ingredient, as well as to methods for treating or alleviating cancer, comprising administering to a subject a therapeutically effective amount a Vibrio cholerae MakA protein.

The invention relates to the technical field of biological detection, and discloses an oligonucleotide composition, a kit and application. The composition disclosed by the invention can be used for rapidly and simultaneously detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus in a sample with high specificity, and has relatively high sensitivity and stability. Compared with the traditional detection methods such as pathogen separation, genome sequencing and the like, the efficiency of identifying and detecting salmonella, shigella, entamoeba histolytica, vibrio cholerae, hepatitis A virus and hepatitis E virus by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

The invention relates to a Vibrio cholerae MakA (motility associated killing factor A) protein for use in therapy, in particular in the treatment or alleviation of cancer such as colorectal cancer. The invention further relates to pharmaceutical compositions comprising a Vibrio cholerae MakA protein as an active ingredient, as well as to methods for treating or alleviating cancer, comprising administering to a subject a therapeutically effective amount a Vibrio cholerae MakA protein.

Provided are modified Vibrio natriegens (V. natriegens ) that are plasmid transformation competent at room temperature. The modified V. natriegens are characterized by having a disruption of V. natriegens dns gene, a genomic insertion of a Vibrio cholerae tfoX gene, and a disruption of a heterologous LacI gene. The combination of the disruption of V. natriegens dns gene, the genomic insertion of a Vibrio cholerae tfoX gene, and the disruption of the heterologous LacI gene provides for enhanced plasmid transformation efficiency, relative to plasmid transformation efficiency using modified V. natriegens that include the disruption of the V. natriegens dns gene and an introduced a Vibrio cholerae tfoX gene, but not the disruption of the heterologous LacI gene. Cell culture media used to generate the modified bacteria are provided. Kits that include the modified bacteria or the cell culture media are also provided.

Formulations of lytic bacteriophages against Vibrio cells and use thereof for preventing or treating bacterial infections, including those by bacteria of genus Vibrio, for example, V. cholerae. The bacteriophage compositions contain three strains of lytic bacteriophage against Vibrio cells. The compositions include Vibrio bacteriophages ICP1, ICP2, and ICP3; and an excipient coating disposed on the bacteriophages, the coating including hydroxypropyl β-cyclodextrin, HPC, HPMC, HPMC-AS, or PVP-VA.

The invention relates to a rapid vibrio parahaemolyticus detection kit based on a one-tube method RPA-CRISPR / Cas12a and a detection method of the rapid vibrio parahaemolyticus detection kit. A specific RPA (recombinase polymerase amplification) primer and CRISPR crRNA (clustered regularly interspaced short palindromic repeats) are designed by taking a ToxR gene of vibrio parahaemolyticus as According to the established one-tube method RPA-CRISPR / Cas12a detection system, the risk of aerosol pollution caused by a cover opening process is effectively avoided. The method disclosed by the invention is high in specificity, and has no cross reaction with related vibrios such as vibrio vulnificus, vibrio alginolyticus, vibrio cholerae, vibrio harveyi and vibrio anguillarum, and other aquatic pathogenic bacteria such as pseudomonas aeruginosa and pseudomonas plecoglossicida; the sensitivity is high, and the lowest detection limit of genome DNA is as low as 1 pg / mu L; the detection is rapid, and the whole process of nucleic acid amplification and fluorescence detection only needs about 30 minutes. The kit and the method do not need complex instruments and equipment, are simple to operate, are suitable for on-site rapid detection of aquatic product samples in a base layer, and have important application values in early screening, epidemiological investigation and comprehensive prevention and control of vibrio parahaemolyticus.

This invention discloses a dual-channel quadruple ddPCR detection method and kit for the simultaneous detection of diarrheal bacteria in wastewater based on a proportional fluorescent probe strategy. Compared with traditional quadruple detection methods using four different fluorescent channels, the quadruple ddPCR detection method of this invention achieves quadruple detection only through the difference in probe concentration within two fluorescent channels. Wastewater, as a key interface between excrement and the environment, is not only an important site for the storage and transmission of pathogens but also a sentinel for early warning. The dual-channel quadruple ddPCR method established in this invention can simultaneously detect Salmonella in wastewater. Salmonella spp., SM), Shigella ( Shigella spp., SH), Vibrio parahaemolyticus ( V.parahaemolyticus, VP) and Vibrio cholerae ( Vibrio cholerae, VC (Vacuum-Containing Microorganisms) not only improves wastewater monitoring technology and methods, but also enables the detection of potential epidemic risks before disease outbreaks occur, allowing for timely implementation of public health prevention and control measures.