57 results about "Vibrio cholera" patented technology

The invention belongs to the technical field of molecular biology, and particularly relates to a nucleic acid sequence combination for simultaneously detecting various pathogenic vibrios, an RT-PCR (reverse transcription-polymerase chain reaction) kit and a use method of the RT-PCR kit, and the nucleic acid sequence combination is shown as SEQ ID No.1-SEQ ID No.19. The RT-PCR kit comprises RT-PCR primer probes, the primer probes comprise forward and reverse amplification primers and detection probes respectively aiming at vibrio cholerae, vibrio parahaemolyticus, vibrio fluvialis, vibrio mimicus, vibrio vulnificus and vibrio alginolyticus, and corresponding sequences in SEQ ID No.1-SEQ ID No.19 are adopted. The kit disclosed by the invention can be used for rapidly detecting and identifying the six vibrios at the same time; the kit has high accuracy, specificity and sensitivity, and is suitable for clinical detection of infected samples.

According to the application of the bacteriophage to reduction of cholera transmission, the bacteriophage phi YSZKA is preserved in the China Center for Type Culture Collection on August 1, 2018, the preservation number is CCTCC NO: M 2018513, and the bacteriophage phi YSZKA is classified and named as klebsiella pneumoniae bacteriophage phi YSZKA. The method solves the problems of secondary pollution, wide transmission of vibrio cholerae and the like of the traditional split bacteriophage. The application of the bacteriophage can reduce the toxin production efficiency of vibrio cholerae in oceans, soil and sewers, thereby providing more possibilities for prevention and control of cholera epidemic situation and alleviation of cholera symptoms.

The invention discloses recombinant bacillus subtilis with the surface displaying Edwardsiella tarda outer membrane protein OmpA and vibrio cholerae enterotoxin CTB fusion protein, a construction method and application, spore capsid protein CotY serves as an anchor point, coding genes of OmpA and CTB are recombined into a genome of bacillus subtilis 168 in a homologous double cross mode, and the recombinant bacillus subtilis is obtained. The recombinant bacillus subtilis can be stably inherited in the bacillus subtilis and displays edwardsiella tarda outer membrane protein OmpA and vibrio cholerae enterotoxin CTB on the spore surface; after fishes and mammals orally immunize the Edwardsiella tarda, the survival time and the survival rate of bodies infected with the Edwardsiella tarda are remarkably improved; the recombinant bacillus subtilis is used as an oral vaccine type micro-ecological immune preparation for preventing and treating Edwardsiella tarda infection of fishes and mammals, the immune procedure is remarkably simplified, the immune stress is reduced, the labor cost is saved, the effective action concentration in the intestinal tract is ensured, and the organism is induced to generate specific immune response.

The invention provides a primer combination of a multiple detection kit for food-borne pathogenic bacteria and the multiple detection kit. The primer combination comprises probes and primers for detecting nine food-borne pathogenic bacteria: vibrio cholerae general type, vibrio cholerae O1 type, vibrio cholerae O139 type, vibrio parahaemolyticus, salmonella, yersinia enterocolitica, campylobacter, listeria monocytogenes and enterobacter sakakaakii, and the sequence is shown as SEQ1-31; the probe and the primer pair are used for detecting nine characteristic genes, namely a shigella universal type: ipaH, a diarrheogenic Escherichia coli universal type: eltA, sta3, sta and aggR, and enterohemorrhagic Escherichia coli: stx1, stx2, escV and rfbE, and the sequences of the probe and the primer pair are shown as SEQ32-64. According to the invention, by combining a digital PCR multiple coding technology, the detection of 12 food-borne pathogenic bacteria can be realized, and the sensitivity, the specificity and the accuracy are relatively good.

The invention discloses a kit for simultaneously detecting 16 food-borne pathogenic bacteria. Each group of probes is designed based on bacterial classification, virulence gene specificity or serotype marks of the pathogenic bacteria; a group A probe vibrio detection group is used for detecting vibrio cholerae universal type, vibrio cholerae O1 serotype, vibrio cholerae O139 serotype and vibrio parahaemolyticus; the B group of probe invasive enterobacteriaceae pathogen group is used for detecting escherichia coli O157: H7, shigella, salmonella and yersinia small intestine; the C group probe escherichia coli pathotype detection group is used for detecting enterotoxigenic escherichia coli ETEC, enteroinvasive escherichia coli EIEC, enteroaggregative adherent escherichia coli EAEC and pathogenic escherichia coli EPEC; and the D group of probes are used for detecting campylobacter jejuni, campylobacter colons, listeria monocytogenes and enterohemorrhagic escherichia coli EHEC in the campylobacter and toxin type pathogen group.

The invention belongs to the technical field of microorganisms, and particularly discloses an ethanol phase extract of rumex madaio roots and application of the ethanol phase extract. An ethanol phase extract RmEE is extracted from rumex madaio roots through a 55-95% ethanol solvent extraction method, and the ethanol phase extract RmEE is further separated and purified to obtain a purified component RmEE-F2. The ethanol phase extract RmEE and the purified component RmEE-F2 of the ethanol phase extract RmEE can inhibit staphylococcus aureus and vibrio cholerae, and can be used as bacteriostatic agents. The ethanol phase extract of the rumex madaio root can inhibit the pollution of staphylococcus aureus and vibrio cholerae in meat of crucian and penaeus vannamei which are stored at low temperature (4 DEG C), and can be applied to aquatic products; the method can also be used for preparing food or medicines, and the prepared food or medicines also have an antibacterial effect.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The invention provides a primer combination for a multiple detection kit for marine pathogenic bacteria and the multiple detection kit. The primer combination comprises primer pairs and probes which are used for detecting 13 marine pathogenic bacteria: vibrio alginolyticus, vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio marine fish, vibrio river, vibrio menivibrio, vibrio mimicus, vibrio harveyi, vibrio occultus, vibrio horii, plesiomonas and aeromonas, and the sequence of the primer combination is as shown in SEQ1-SEQ39. According to the present invention, the genomic DNA of 13 marine pathogenic bacteria is identified and detected in one digital PCR reaction through the ultra-multiple digital PCR fluorescence coding technology, and the kit not only covers the common vibrio species such as vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus and the like, but also can identify the sea area vibrio, vibrio holii, vibrio occultus and the like, and the kit has good sensitivity, specificity and accuracy.

The invention relates to a method for producing a water environment protective agent by fermenting hermetia illucens larva slurry, and belongs to the technical field of microbial fermentation. According to the present invention, the hermetia illucens larvae are adopted as the main raw material, the main raw material is ground into the slurry through the colloid mill, the small amount of the carbon source is supplemented, the Phaffia rhodozyma CGMCC NO.29217, the Bacillus coagulans CGMCC NO.5233 and the Clostridium butyricum CGMCC NO.4729 are subjected to the mixed bacteria synergistic fermentation, and the produced product can be widely used in the aquaculture and other application scenarios. Fermented hermetia illucens larva serous fluid is rich in astaxanthin, a fermented product has obvious acid fragrance and no peculiar smell, the total number of probiotics is larger than or equal to 5.0 * 10 < 8 > cfu / g, the pH value is smaller than 4.50, and the content of small molecule active polypeptide (acid soluble protein) of the fermented product is increased by 2.5-3 times compared with that before fermentation. Bacillus subtilis CGMCC (B) 63501 is used as indicator bacteria to detect that the content of bacteriostatic active substances after fermentation of hermetia illucens larva serous fluid is larger than 1.0 g / kg, pathogenic bacteria such as salmonella, vibrio parahaemolyticus and vibrio cholerae are not detected, and normal-temperature storage can be achieved. The preparation method is low in production cost, easy to operate, safe and free of residues, and the economic benefits of aquaculture can be improved.

The application provides a new bacteriophage for preventing and treating vibrio diseases of aquatic animals and application thereof, the bacteriophage is preserved in China Center for Type Culture Collection on September 4, 2020, and the preservation number is CCTCC M 2020465. The bacteriophage is a new species of bacteriophage of the Caudovirales class and Queuovirinae subfamily, the whole genome of the bacteriophage is OP331229.1 in the NCBI official website GeneBank. The bacteriophage has a wide phage spectrum, can crack 6 different species and genera of vibrio at the same time, can crack 45 strains of Vibrio parahaemolyticus, 22 strains of Vibrio harveyi, 17 strains of Vibrio alginolyticus, 13 strains of Vibrio vulnificus, 11 strains of Vibrio campbellii and 9 strains of Vibrio cholerae at the same time, can more effectively solve the problem of multiple bacterial disease infections in aquaculture, and can be used as a microecological preparation for preventing and treating vibrio diseases such as Vibrio parahaemolyticus, Vibrio harveyi and Vibrio alginolyticus of aquatic animals.

The application belongs to the technical field of microorganisms, and specifically discloses a methanol phase extract of fruit of Phyllanthus emblica and application thereof. The methanol phase extract PeME is extracted from the fruit of Phyllanthus emblica by a methanol-chloroform extraction method, and a purified component PeME-F3 is obtained by further separation and purification of the methanol phase extract. The methanol phase extract PeME and the purified component PeME-F3 can inhibit Vibrio cholerae and Staphylococcus aureus, and can be used as bacteriostatic agents. The methanol phase extract of the fruit of Phyllanthus emblica can inhibit contamination of Vibrio cholerae and Staphylococcus aureus in crucian carp and Penaeus vannamei stored at low temperature, and can be applied to aquatic products; the methanol phase extract can also be used for preparation of food or medicine, and the prepared food or medicine also has the bacteriostatic effect.

The invention relates to a primer and probe combination, a kit and a method for detecting gastrointestinal tract pathogenic bacteria. The gastrointestinal pathogenic bacteria comprise vibrio cholerae, vibrio parahaemolyticus, salmonella, shigella, diarrheagenic escherichia coli, campylobacter, yersinia enterocolons, clostridium difficile, cronobacter sakazakii, aeromonas hydrophila, plesiomonas shigelloides and vibrio fluvialis. The primer and the probe group can be independently used for PCR amplification detection and can be combined in the same reaction system and reaction procedure for multiple PCR amplification detection, and the primers and the probes aiming at different pathogenic bacteria cannot interfere with each other during multiple PCR amplification detection. The primer and probe combination covers various types of gastrointestinal pathogenic bacteria, and is high in sensitivity, low in detection limit, good in specificity and wide in application prospect.

The invention discloses a detection method for detecting enteropathogenic bacteria, and relates to the field of molecular biology, specific genes are searched in genomes of salmonella, shigella, vibrio parahaemolyticus, campylobacter jejuni, diarrheagenic escherichia coli, vibrio cholerae and staphylococcus aureus, a beta-actin gene is adopted as a reference gene, and the enteropathogenic bacteria are detected. Primer and probe sequences are designed, a probe group consisting of the primer and probe sequences is used for carrying out real-time fluorescent PCR amplification reaction on nucleic acid in a detected sample, then melting curve analysis is carried out, and whether the pathogenic bacteria exist in the nucleic acid sequence to be detected or not can be judged according to a Tm value. The detection method provided by the invention has the advantages of rapidness, high throughput, accuracy and high sensitivity.

The invention relates to the field of biological detection, in particular to a primer probe set, a detection reagent and application of the primer probe set and the detection reagent in detection of virulent pathogenic bacteria. The invention provides a primer probe group. The primer probe group comprises a primer probe group used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae, salmonella typhimurium and staphylococcus aureus, wherein the primer probe group is used for amplifying bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum-like bacteria, brucella, vibrio cholerae and salmonella typhimurium. The invention establishes a primer probe combination for rapidly and sensitively detecting various virulent pathogenic bacteria and a kit thereof, aiming at eight virulent pathogenic bacteria such as bacillus anthracis, Francisella tularensis, bacillus plague, rhinotrichum, vibrio cholerae, brucella, salmonella typhimurium and staphylococcus aureus. The kit is stored in a freeze-dried state, adaptive sample lysate can directly expand different types of samples, and the kit has the advantages of being good in specificity, high in sensitivity, short in detection time, good in accuracy, good in stability after redissolution and good in compatibility.

The invention discloses a bacteriophage protein Gp61 and application thereof in preparation of a preparation for inhibiting vibrio cholerae colonization and toxin secretion. A specific bacteriophage gene bank targeting vibrio cholerae is used as a screening object, protein capable of inhibiting vibrio cholerae colonization and toxin secretion in the interaction process of the bacteriophage gene bank and a host is found, new regulatory protein Gp61 for inhibiting vibrio cholerae colonization or virulence is found and identified, functional information of unknown functional protein of bacteriophage can be supplemented, and the effect of inhibiting vibrio cholerae colonization or virulence is achieved. Main biological functions of a host influenced by the vibrio cholerae can be determined, and a basis is provided for vibrio cholerae prevention and control and virulence control.

The invention discloses a microcos paniculata methanol phase extract with antibacterial and bacteriostatic activity and application thereof, and belongs to the technical field of microorganisms. The microcos paniculata methanol phase extract is prepared by a methanol-chloroform extraction method, and can inhibit various common pathogenic bacteria such as staphylococcus aureus, bacillus cereus, pseudomonas aeruginosa, shigella dysenteriae, vibrio alginolyticus, vibrio cholerae, vibrio parahaemolyticus, vibrio harveyi, vibrio menisei and vibrio fluvialis in vitro; the compound also has the effect of inhibiting pathogenic vibrio parahaemolyticus in vivo, can effectively remove vibrio parahaemolyticus in intestinal tracts of mice, eliminate damage of infection of the vibrio parahaemolyticus to ileum tissues of the mice and reverse diversity imbalance of ileum flora of the mice caused by infection of the vibrio parahaemolyticus, can be used for preparing antibacterial and bacteriostatic foods and medicines, and has a wide application prospect.

Described herein are compositions, methods, and kits for detecting diarrhea causing pathogens from patient, food, or environmental samples. One embodiment described herein is primer pairs and probes for multiplex polymerase chain reaction (PCR) based assays for the detection of diarrhea causing pathogens, such as Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Plesiomonas shigelloides, Yersinia enterocolitica, or Aeromonas hydrophila. Other embodiments include methods and kits for detecting diarrhea causing pathogens.

The invention discloses a nucleic acid detection kit for typhoid fever, paratyphoid fever, cholera, shigella and dysentery. The nucleic acid detection kit comprises a multiple fluorescent PCR detection system, and the multiple fluorescent PCR detection system comprises a typhoid fever salmonella primer probe, a paratyphoid fever salmonella primer probe, a vibrio cholera primer probe, a dysentery amoeba primer probe and a shigella primer probe; according to the kit, specific and conservative characteristic genes of different bacteria species are selected for primer probe design, so that the specificity, the accuracy and the sensitivity of detection are ensured.

The invention discloses bacillus velezensis Jcl-10 and application of the bacillus velezensis Jcl-10 in prevention and treatment of vibrio cholerae. The strain Jcl-10 is preserved in the China Center for Type Culture Collection, the preservation time is January 25, 2024, and the preservation number is CCTCC N: M 2024212. The bacillus velezensis Jcl-10 disclosed by the invention is identified as a gram positive bacterium after being subjected to gram staining. The bacterial strain can produce antibacterial peptide substances, antibacterial protein substances, polyketide synthetase substances and the like with bacteriostatic action, and can be well applied to prevention and treatment of aquatic diseases caused by vibrio cholerae. The Jcl-10 is added into the aquatic feed, so that the morbidity of aquaculture animals can be effectively reduced, and the economic yield of the aquaculture animals can be increased. In conclusion, the bacillus velezensis Jcl-10 disclosed by the invention has outstanding performance in the prevention and treatment of vibrio cholerae, and provides a powerful guarantee for continuous and healthy culture of aquatic products.

Disclosed are a chemical synthesis method for Vibrio cholerae serotype O100 O-antigen oligosaccharide, and a use, belonging to the technical field of chemistry. The present invention uses three monosaccharide building blocks and five kinds of carboxylic acid derivative, and under the effects of solvent, temperature and neighboring group participation, through orthogonal protection, selective assembly and amide coupling, five Vibrio cholerae serotype O100 O-antigen oligosaccharide fragments are synthesized. Using the synthesized oligosaccharide fragments, combined with NMR analysis and carbohydrate chip technology, the absolute configuration and immunological function of the 3,5-dihydroxyhexanoyl group in the O-antigen trisaccharide are clarified, providing a theoretical basis for further structure-activity research and minimal antigen epitope screening. The present invention has promising applications in the synthesis of Vibrio cholerae glycoconjugate vaccines and the development of new drugs.

The present application relates to a kind of pseudomonas aeruginosa engineering bacteria DUEC construction method and application.The DUEC engineering strain construction method of the present application is: response to kinase coding gene retS and sheath protein coding gene tssB2, tssB3 Knockout, H1-T6SS secretory effector protein Tse1-Tse4, Tse6-Tse8 catalytic site mutation, Tse5 Coding gene knockout.DUEC engineering strain can not only constitutively express and assemble H1-T6SS, but also can respond to adjacent sister cell source T6SS attack, assemble H1-T6SS at the position of being attacked.The present application constructs a series of effector protein combination inactivation mutant by gene knockout or catalytic site mutation method.Microscope observation and statistical analysis results show that no single effector protein is necessary for T6SS attack and assembly.By bacterial competition experiment, it is verified that all effector protein inactivation mutant 8eff c Lose T6SS + Vibrio cholerae and T6SS + Aeromonas dhaka's bactericidal activity, but 8eff c Can respond to T6SS physical attack, Tse6 N -Cre is specifically delivered to T6SS + In Vibrio cholerae.

The application discloses a new use of an agricultural enzyme and a method for controlling and inhibiting soil pathogenic bacteria biological pollution, wherein the agricultural enzyme is used as a soil repair agent to prevent or treat soil pathogenic bacteria biological pollution, and the soil pathogenic bacteria are at least one of Klebsiella pneumoniae, Acidovorax xylosoxidans, Streptomyces orientatis, Vibrio cholerae, Riemerella anatipestifer, Clostridium botulinum, Serratia marcescans, Rhodococcus globerulus and Aeromonas salmonicida. The agricultural enzyme is applied to the pathogenic bacteria biological pollution soil to be treated in different ways. The application directly kills the pathogenic bacteria or regulates the soil microbial community through a quorum sensing gene and a virulence factor gene, so that the abundance of the pathogenic bacteria in the soil community is reduced, and the soil pathogenic bacteria biological pollution is reduced. The application is an economic, green, convenient, efficient agricultural soil environment repair technology.

The invention discloses a kit for detecting toxin-producing vibrio cholerae based on a dual-fluorescence RPA (recombinase polymerase amplification) technology and application of the kit. The invention provides a complete set of primers and probes. The complete set of primers and probes comprises a primer Vc-F1, a primer Vc-R4, a probe Vc-P, a primer ctxB-F, a primer ctxB-R and a probe ctxB-P, according to the invention, countless tests are matched with specific retrieval, a section of specific conserved sequence Vc and virulence gene ctxB on a pathogen genome are selected as detection targets, and whether a to-be-detected sample is toxigenic vibrio cholerae or not can be rapidly determined by simultaneously detecting the two targets. According to the invention, a brand new technical support is provided for on-site rapid diagnosis and epidemic disease investigation of vibrio cholerae by applying a dual-fluorescence RPA technology.

The application discloses a recombinant bacillus subtilis of surface display of a fusion protein of Edwardsiella tarda outer membrane protein OmpA and Vibrio cholerae enterotoxin CTB, a construction method and application. A homologous double cross recombinant of coding genes of OmpA and CTB is carried out into a genome of the bacillus subtilis 168 by taking the spore coat protein CotY as an anchor point, so that the recombinant bacillus subtilis capable of stably inheriting in the bacillus subtilis and displaying the Edwardsiella tarda outer membrane protein OmpA and the Vibrio cholerae enterotoxin CTB on a spore surface is obtained. After fish and mammals orally immunize the bacillus subtilis, the survival time and survival rate of the fish and mammals after infection of the Edwardsiella tarda are obviously improved. The recombinant bacillus subtilis is used as an oral vaccine type micro-ecological immunization preparation for preventing and treating the Edwardsiella tarda infection of the fish and mammals, so that the immunization procedure is obviously simplified, the immunization stress is reduced, the labor cost is saved, the effective concentration in the intestinal tract is ensured, and the specific immune response of the body is induced.

The present invention relates to the field of biotechnology and discloses a bacteriophage protein Gp37 and its use in preparing a preparation for inhibiting Vibrio cholerae colonization and toxin secretion. The present invention screens a library of phage genes specifically targeting Vibrio cholerae to identify proteins capable of inhibiting Vibrio cholerae colonization and toxin secretion during interaction with the host. The discovery and identification of a novel regulatory protein, Gp37, that inhibits Vibrio cholerae colonization or virulence not only supplements functional information about unknown phage proteins but also clarifies their primary biological functions influencing the host, providing a foundation for the prevention, control, and virulence control of Vibrio cholerae.

The invention discloses lactobacillus plantarum D27 for antagonizing vibrio cholerae and application thereof, and belongs to the technical field of microorganisms, the lactobacillus plantarum D27 is preserved in China Center for Type Culture Collection (CCTCC) on September 4, 2024, the preservation address is No. 299 on eight road, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC M 20241910. The lactobacillus plantarum D27 disclosed by the invention can effectively antagonize vibrio cholerae, inhibit the formation of a biofilm of the vibrio cholerae and destroy a cell structure of the vibrio cholerae, also has the characteristics of high yield of lactic acid and exopolysaccharide and good self-aggregation and co-aggregation, and can be used for preparing foods or medicines for antagonizing vibrio cholerae pollution; comprising lactobacillus plantarum liquid, lactobacillus plantarum powder, lactobacillus plantarum capsules, lactobacillus plantarum tablets and the like.

The invention relates to a synbiotic enzyme as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture. The synbiotic enzyme is prepared from garlic, brown sugar, radix astragali, fructus schisandrae chinensis, fructus chebulae, lactobacillus hirsuti liquid and water. The synbiotic enzyme disclosed by the invention has a broad-spectrum antibacterial effect, and has a relatively strong inhibition effect on bacterial pathogenic bacteria such as vibrio alginolyticus, listonella anguillarum, edwardsiella ictaluri, aeromonas hydrophila, vibrio parahaemolyticus, aeromonas veronii and vibrio cholerae; the composition can be used for preventing and treating hemorrhagic ascites disease, necrotic canker and other aquaculture diseases caused by bacteria and pathogenic bacteria in aquaculture, can improve the feed conversion rate in aquaculture, and improves the growth and reproduction capability, immunity and culture benefits of aquatic animals.

The present disclosure discloses a specific saccharide fragment for development of Vibrio cholerae vaccines, and belongs to the field of medicine. In the present disclosure, a saccharide fragment related to a trisaccharide of V. cholerae O100 serotype O-antigen is chemically synthesized. Combined with a glycan microarray technology, the structure-activity relationship between different saccharide fragments and antigenicity thereof is evaluated at a molecular level. Glycan microarray screening indicates that 3-hydroxybutyryl is an essential structural feature of the O-antigen. A non-reducing end disaccharide carrying 3-hydroxybutyryl is a potential minimal antigenic epitope, and the disaccharide has strong binding capacity to antibodies and a simple structure, and may serves as a specific saccharide fragment for vaccine development. A glycoconjugate vaccine designed based on the specific saccharide fragment may solve the challenges of difficulty in culturing pathogenic bacteria and heterogeneity of saccharide antigens in naturally extracted polysaccharide vaccines. The present disclosure has bright application prospects in the development of glycoconjugate V. cholerae vaccine, infection detection, and new drug development.

The utility model discloses a telescopic water sampler for sampling vibrio cholerae in a large water body, which is characterized in that a sampler for placing samples is arranged, a hook is mounted on the upper surface of the sampler, and a sealing door is rotationally connected to the front side of the outer surface of the sampler; comprising a built-in bin installed on the inner surface of the collector, an unlocking groove is formed in the front side of the outer surface of the built-in bin, a water suction pipe is connected between the built-in bin and the collector in a nested mode, and meanwhile a sample tank is connected to the inner surface of the built-in bin in a nested mode. A telescopic assembly for mounting the collector is arranged, a water suction pipe and a sample tank are nested and assembled in the collector, the stability of the sample tank is controlled through cooperation of a clamping piece and a reset spring arranged on the sample pipe, and a telescopic pipe in the telescopic assembly is matched with a water inlet pipe, so that water sampling stretching is adjusted, and stretching adjustment of the water sampler is controlled; the clamping connection of the external bin is matched, so that the influence of impurities on sample collection is avoided, and the stability of vibrio cholerae collection is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to application of selenium-enriched lactobacillus reuteri to prevention and treatment of enteropathogenic bacteria infection and promotion of chick growth. The selenium-enriched lactobacillus reuteri is obtained by carrying out selenium-enriched culture on lactobacillus reuteri with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.31975, and the selenium-enriched culture is that the lactobacillus reuteri is placed in a culture medium with the sodium selenite content of 5 to 7mg / mL for culture. The enteropathogenic bacteria comprise vibrio parahaemolyticus, escherichia coli, vibrio cholerae and salmonella typhimurium, and the selenium-enriched lactobacillus reuteri obtained after selenium-enriched culture is stronger in biological function, can remarkably promote growth of chickens, can effectively inhibit growth of pathogenic bacteria, reduces the bacterial infection risk of chicken flocks, improves the breeding benefit, and has a good application prospect. A brand-new, safe and effective solution is provided for the poultry breeding industry.