The invention relates to a complex enrichment medium used for
salmonella,
vibrio parahaemolyticus and comma bacillus and a method for preparing the same. The formulation components of the complex enrichment medium in weight portion are as follows: 0.5 to 1.5 portions of buffer peptone water, 1000 portions of
distilled water, 1.0 to 5.0 portions of glucose, 1.0 to 5.0 portions of
mannitol, 0.03 to 0.06 portion
sodium pyruvate, 0.5 to 2.5 portions of
anhydrous sodium sulfite, 1.0 to 10.0 portions of
sodium citrate, 5.0 to 25.0 portions of sodium
chloride, 1 to 5 portions of cholate, and 0.0005 to 0.0025 portion of
potassium tellurite. The method comprises the following steps: firstly, adding the buffer peptone water and the like into the
distilled water, and sterilizing the mixture after heating and melting; and secondly, cooling the mixture to 50 DEG C, and then adding the
potassium tellurite into the mixture to be mixed evenly. The complex enrichment medium can integrate two steps of primary enrichment and selective enrichment of bacterial detection to shorten the enrichment time to 24 hours, can perform enrichment on three target pathogens at the same time, and can inhibit the growth of other microorganisms.