Streptococcus mutans detection method based on quorum sensing system and application of streptococcus mutans detection method

Abstract

The invention discloses a streptococcus mutans detection method based on a quorum sensing system and application of the streptococcus mutans detection method, and belongs to the technical field of genetic engineering. The detection method is established based on the quorum sensing system; elements of the sensing system comprise a gene PelB-comD compounded by a signal peptide of escherichia coli and a histidine kinase receptor membrane protein gene, an intracellular reaction regulation protein gene comE, and a gene nlmC-sfGFP compounded by a promoter nlmC, regulated and activated by comE, and a green fluorescence signal reporter gene sfGFP. According to the detection method, the signal molecule CSP secreted and expressed by the streptococcus mutans is subjected to specific recognition response through the sensing system, and then the streptococcus mutans is detected. The detection method disclosed by the invention can specifically recognize and detect streptococcus mutans, has the characteristics of convenience in operation and low detection cost, and can be applied to decayed tooth detection so as to evaluate the health condition of teeth.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for detecting Streptococcus mutans based on a quorum sensing system and an application thereof. Background technique [0002] Streptococcus mutans is a bacterium that grows in the oral cavity. It has the characteristics of secreting acidic substances to corrode tooth enamel. It is one of the main cariogenic bacteria in the oral cavity and is often found in dental plaque. Streptococcus mutans can corrode through the acidic substances produced by metabolism, and then lead to the occurrence of dental caries, which has been widely studied in the field of stomatology. [0003] However, the current method for detecting Streptococcus mutans is relatively single, and requires the cooperation of expensive instruments and high-precision operations. For example, the PCR technology currently mainly used for detecting Streptococcus mutans is relatively complicated to oper...

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Problems solved by technology

[0004] In addition, although PCR technology has the characteristics of high detection sensitivity and specificity, in this way, it also has a magnifying effect on detection errors, and it is prone to inaccurate results such as false positives. Therefore, other detection technologies are urgently needed to supplement. To ensure the accuracy of the test results

[0005] Quorum sensing system detection is a relatively economical and easy-to-operate detection method, but it has not been applied to the detection of Streptococcus mutans at present, and no suitable quorum sensing system components have been found, and how to construct it is still a mystery. is also difficult

Therefore, so far, no effective method for supplementary detection of Streptococcus mutans has been found.

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