34 results about "Membrane Protein Gene" patented technology

The invention discloses a cell membrane protein replacement type oncolytic virus vector and application thereof. The cell membrane protein replacement type oncolytic virus vector is rhabdoviridae virus, and a nucleotide sequence for coding G protein in a genome of the rhabdoviridae virus is replaced by a nucleotide sequence for coding an antibody and a nucleotide sequence for coding a spike protein truncation of coronavirus. The invention also discloses a construction method of the cell membrane protein replacement type oncolytic virus vector for expressing the antibody, the non-replicated virus vector is used for expressing the antibody sequence for the first time, and meanwhile, the novel coronavirus cell membrane protein is embedded into the virus surface, so that the cell membrane protein replacement type oncolytic virus vector can be rapidly produced in a suspension cell in a large scale; through removal of virus cell membrane protein genes, the virus cell membrane protein genes cannot be continuously replicated in vivo, so that the safety of the virus cell membrane protein genes is ensured, the tumor immunosuppression condition is improved, and an organism can be stimulated to generate a neutralizing antibody for resisting new coronavirus while tumor cells are killed.

The invention belongs to the field of genetic engineering and biological treatment, and provides an eukaryotic expression vector, gram-negative bacteria containing the eukaryotic expression vector and application of the eukaryotic expression vector in preparation of tumor drugs. By selecting and combining a phage-derived promoter, a specific phage RNA polymerase gene, a membrane rupture protein gene and an eukaryotic expression drug protein gene, an eukaryotic expression vector is divided into three modules: (1) a phage-derived RNA polymerase gene transcription module; (2) a membrane rupture protein gene expression module; and (3) a eukaryotic drug protein gene expression module. Through organic combination of the three modules, the eukaryotic drug delivery system is constructed, and the system and method for stably and efficiently performing eukaryotic drug protein expression, drug delivery and drug killing are realized.

Provided are a recombinant Escherichia coli with improved ability to secrete extracellular endoglucanase (BcsZ) and a construction method therefor. The recombinant Escherichia coli is constructed by using Escherichia coli JBZ-DH5α as a starting strain and inserting a membrane protein coding gene. The membrane protein gene is one of the following coding genes: a coding gene of zinc transporter permease znuB, a coding gene of DUF1449 family inner membrane protein ylaC, and a coding gene of UPF0410 family inner membrane protein ymgE.

The application belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec61 alpha gene and application of dsRNA of the membrane protein Sec61 alpha gene in pest control. After cloning and sequencing of the Sec61 alpha gene of Locusta migratoria, the sequence of the Sec61 alpha gene of SEQ ID NO:1 is obtained; then the gene fragment of SEQ ID NO:2 is selected for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the Locusta migratoria, specific Sec61 alpha genes can be silenced, and the Locusta migratoria stops feeding and successively dies before molting. The application provides a new specific molecular target for pest control based on RNA interference, and has very important application value.

The invention discloses a fiveleaf gynostemma herb membrane protein gene GpAnnexin, a recombinant vector, a preparation method and application of the fiveleaf gynostemma herb membrane protein gene GpAnnexin. The sequence of the fiveleaf gynostemma herb membrane protein gene GpAnnexin is shown as SEQ ID NO. 1. The invention also provides a recombinant vector of the gynostemma pentaphyllum membrane protein gene GpAnnexin, and a preparation method of the recombinant vector, and the preparation method comprises the following steps: 1) carrying out double enzyme digestion on pCY-35D-GFP plasmid by using SacI enzyme and SalI enzyme to obtain a pCY-35D-GFP linearized vector; and (2) carrying out homologous recombination reaction on the cDNA full-length sequence fragment of the gynostemma pentaphylla membrane protein gene GpAnnexin and a pCY-35D-GFP linearized vector, converting the reaction product into DH5 alpha cell competence, screening positive clone cells on an LB culture medium containing kanamycin, and extracting plasmids after the positive clone cells are verified to be correct through sequencing, thereby obtaining the pCY-35D-GFP-GpAnnexin recombinant vector. The gynostemma pentaphylla membrane protein gene GpAnnexin provided by the invention can improve the tolerance of plants to cadmium and salt stress, and provides a new thought for cultivating plants with high cadmium enrichment and salt tolerance.

The present application relates to the field of genetic engineering and biological therapy, and provides a eukaryotic expression vector, a gram-negative bacterium containing the eukaryotic expression vector, and a use of the eukaryotic expression vector in the preparation of antitumor drugs and the treatment of tumors. By selecting and combining a phage-derived promoter, a specific phage RNA polymerase gene, a membrane-disrupting protein gene and a eukaryotic expression drug protein gene, the eukaryotic expression vector is divided into three modules: (1) a phage-derived RNA polymerase gene transcription module; (2) a membrane-disrupting protein gene expression module; and (3) a eukaryotic drug protein gene expression module. By means of organic combination of the three modules, a eukaryotic drug delivery system is constructed, and a system and method for stable and efficient eukaryotic drug protein expression, drug delivery and drug killing are implemented.

The application belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec63 gene and application of dsRNA of the membrane protein Sec63 gene in pest control. After cloning and sequencing of the Sec63 gene of Locusta migratoria, the sequence of the Sec63 gene of SEQ ID NO:1 is obtained; then the gene fragment of SEQ ID NO:2 is selected for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the Locusta migratoria, specific Sec63 genes can be silenced, so that the Locusta migratoria cannot successfully molt and dies when molting. The application provides a new specific molecular target for pest control based on RNA interference and a new technical approach for pest control.

The invention discloses a preparation method and application of ackermania mucophilia outer membrane protein transgenic tomatoes. The preparation method provided by the invention comprises the following steps: transforming a codon-optimized ackermann mucophile outer membrane protein gene into competent cells through a cloning vector, and infecting a cultured tomato explant by adopting the transformed competent cells to obtain the ackermann mucophile outer membrane protein. And culturing the transgenic tomato expressing the ackermann mucophile outer membrane protein by adopting the infected explant. According to the preparation method, the AMUC1100 protein can be expressed in tomatoes, the tomato fruits are used for preventing or treating the metabolic dysfunction related fatty liver diseases, the treatment effect of the AMUC1100 protein can be obtained, the functions of the tomatoes can be coordinated, and the effect of preventing or treating the metabolic dysfunction related fatty liver diseases is achieved. A new scheme and strategy are provided for prevention or treatment of metabolic dysfunction related fatty liver diseases; in addition, the problems of infection risk in viable bacteria treatment, high viable bacteria storage and transportation cost and the like are solved.

The invention belongs to the field of biotechnology and agricultural pest control, and particularly relates to a membrane protein Sec63 gene and application of dsRNA of the membrane protein Sec63 gene in pest control. According to the invention, the Sec63 gene of migratory locust is cloned and sequenced to obtain the Sec63 gene of which the sequence is SEQ ID NO: 1; and then the gene segment with the sequence of SEQ ID NO: 2 is selected and used for synthesis of double-stranded RNA (dsRNA). After the dsRNA of the gene is injected into the body cavity of the migratory locust, the specific Sec63 gene can be silenced, so that the migratory locust cannot smoothly exuviate during ecdysis and die. A novel specific molecular target is provided for pest control based on RNA interference, and a novel technical approach is provided for pest control.

The invention belongs to the field of bioengineering, and particularly relates to a construction method and application of a transgenic mouse for screening tissue cell membrane protein in vivo. Aiming at the problems of low in-vivo research flux, unstable library, limited tissue applicability and the like of the traditional membrane protein gene, sgRNA library plasmids are constructed by designing sgRNA of a membrane protein target gene and a control gene; three-positive-mouse containing Cas9, UBC-CreERT2 and sgRNA libraries is obtained through embryo microinjection and hybridization, genotype identification, inducer regulation and control, disease modeling, multi-tissue sample treatment and NGS analysis technical systems are established in a matched mode, and membrane protein gene in-vivo high-throughput screening is achieved. The model can guarantee library stability and screening reliability, covers multi-tissue research scenes, links in-vitro screening and in-vivo verification and is suitable for membrane protein function analysis and disease target mining, the technical process can be popularized to other gene families, and systematic biological research is promoted.

The present invention relates to the field of biomedical technology, and in particular to a probiotic that produces a glucagon-like peptide-1 analog and its application in the treatment of type 2 diabetes (T2DM) and obesity. E. coli Nissle1917 The engineered bacteria can secrete a glucagon-like peptide-1 (GLP-1) analogue that can penetrate the intestinal barrier, wherein the engineered bacteria knock out the membrane protein LPP gene, and the GLP-1 analogue that can penetrate the intestinal barrier is shown in SEQ ID NO.2. The present invention successfully constructs a glucagon-like peptide-1 (GLP-1) analogue that can secrete a glucagon-like peptide-1 (GLP-1) analogue that can penetrate the intestinal barrier. Nissle1917 ΔLPP (EcN‑P) can be applied to the development of fermentation strains, the preparation of probiotic tablets with weight loss function, and the production of drugs with blood sugar control function.

The present application relates to the technical field of veterinary biological products, and particularly relates to a C-type chicken infectious rhinitis subunit vaccine and a preparation method and application thereof. The present application uses Region 2 (abbreviated as TP5-BP5-BS-C-R2) of the outer membrane protein HMTp210 gene of the C-type chicken infectious rhinitis containing an immune enhancement sequence fusion of TP5-BP5-BS as an immunogen to prepare a subunit vaccine. The C-type chicken infectious rhinitis subunit vaccine prepared by the method of the present application has high safety, overcomes the side reactions such as injection site swelling caused by the whole bacteria inactivated vaccine, and can quickly induce the immune animals to produce high levels of neutralizing antibodies, realizes the efficient immune protection of the C-type chicken infectious rhinitis, and the immune effect is better than that of the C-type rhinitis subunit vaccine and the whole bacteria inactivated vaccine without the immune enhancement sequence. Moreover, the preparation process of the subunit vaccine is simple, the cost is low, and the subunit vaccine is easy to mass-produce.

The invention belongs to the field of bioengineering, and particularly relates to a construction method and application of a transgenic mouse for in-vivo screening of a T cell membrane protein gene. Aiming at the problems of low in-vivo screening research efficiency, library instability, tissue applicability limitation and the like of traditional T cell membrane protein genes, sgRNA library plasmids are constructed by designing sgRNA of a T cell membrane protein target gene and a control gene; three positive mice containing Cas9, Ubc-CreERT2 and sgRNA libraries are obtained through embryo microinjection and hybridization, genotype identification, inducer regulation and control, disease modeling, multi-tissue sample treatment and NGS analysis technical systems are established in a matched mode, and in-vivo multi-tissue sample high-throughput screening of T cell membrane protein genes is achieved. The model can guarantee library stability and screening reliability, covers multi-tissue research scenes, links in-vitro screening and in-vivo verification and is suitable for T cell membrane protein gene function analysis and disease target mining, the technical process can be popularized to other gene families, and systematic biological research is promoted.

The present application belongs to the technical field of bioengineering, and relates to a wheat secretory carrier-associated membrane protein TaSCAMP6 and application thereof. The amino acid sequence of the wheat secretory carrier-associated membrane protein TaSCAMP6 provided by the present application is shown as SEQ ID NO:2; the nucleotide sequence of the wheat secretory carrier-associated membrane protein TaSCAMP6 gene is shown as SEQ ID NO:1; the function of the TaSCAMP6 gene is studied by a genetic method in the present application, a transgene is carried out in a Fielder wild type material, a TaSCAMP6 overexpression plant is obtained, and it is found through verification that the TaSCAMP6 overexpression enhances the resistance to Puccinia striiformis, and it is determined that the gene plays a positive regulation role in the wheat resistance to the stripe rust, thereby providing a new gene resource for the wheat breeding against the stripe rust.

The application discloses application of a feather needle grass unknown function domain 679 membrane protein gene SpDUF679 in plant drought stress tolerance, and belongs to the technical field of genetic engineering. The CDS sequence of the unknown function domain 679 membrane protein gene SpDUF679 is shown as SEQ ID NO. 5, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 6. The application finds a new unknown function domain membrane protein 679 of feather needle grass, names it as SpDUF679, successfully constructs a plant expression vector of the gene, and verifies that the gene has important functions of being capable of enhancing the drought stress resistance of plants in transgenic plants. The application has important significance for revealing the stress resistance mechanism of feather needle grass, enriching the theory of plant stress resistance molecular biology, and improving the stress resistance of plants.

The invention discloses a genetically modified bovine mycobacterium attenuated strain as well as a construction method and application thereof. Reconstruction is carried out by using a homologous recombination principle, and gene knockout of a genome RD1 region and membrane protein gene insertion are realized, so that an attenuated live vaccine candidate strain is constructed. The compound can be used as an immunotherapeutic agent or a tuberculosis attenuated live vaccine for bladder cancer treatment. Based on a mycobacterium bovis virulent strain AF2122 / 97, an RD1 region of the mycobacterium bovis virulent strain AF2122 / 97 is knocked out through gene modification so as to reduce the toxicity of the mycobacterium bovis virulent strain AF2122 / 97, but cell wall antigens (such as MPB70 / 83) are retained, and meanwhile, deletion region related antigen fragments are refilled and inserted so as to enhance the immunogenicity of the mycobacterium bovis virulent strain AF2122 / 97.

The present invention relates to the field of biological vaccine research and development technology, and in particular, to a VSV vector-based highly pathogenic H5 subtype avian influenza inactivated vaccine and a method for preparing same. The method comprises: using a vesicular stomatitis virus with the deletion of the surface envelope protein gene as a vector, inserting the NA gene and the HA gene of an H5 subtype highly pathogenic avian influenza virus strain between the M gene and the L gene of the vector in the form of an expression cassette, and acquiring a recombinant virus by means of rescue via reverse genetic technology; inoculating the recombinant virus into susceptible cells, culturing for replication and proliferation, and then harvesting a viral solution; inactivating the viral solution to acquire an inactivated viral solution; and adding the inactivated viral solution to a pharmaceutically or veterinarily acceptable carrier, excipient, medium, or adjuvant to acquire an inactivated vaccine. The provided H5 subtype avian influenza inactivated vaccine can induce a high hemagglutination inhibition titer of avian influenza virus on day 21 after chick immunization. The provided inactivated vaccine has good safety and immunogenicity.

The invention belongs to the field of biology, and discloses a plasmid which contains a haemophilus parasuis Omp Am outer membrane protein gene and a mycoplasma hyopneumoniae membrane protein gene. The mycoplasma hyopneumoniae membrane protein gene is one or a combination of more of a mycoplasma hyopneumoniae membrane protein P46 gene, a mycoplasma hyopneumoniae membrane protein P65 gene and a mycoplasma hyopneumoniae membrane protein P159 gene. The plasmid contains an Omp Am outer membrane protein gene and a mycoplasma hyopneumoniae membrane protein gene, recombinant haemophilus parasuis is prepared from a target gene amplified from the vector through a primer in a natural transformation mode, one or more of Mhp antigen proteins such as P46 protein, P65 protein and P159 protein can be effectively expressed, and the effect of double immunization is achieved. Meanwhile, the invention also provides recombinant haemophilus parasuis and application thereof.

A constitutive expression vector containing dual bacteriophage-derived promoters, a gram-negative bacterium containing the expression vector, and uses thereof in preparation of tumor drugs and treatment of tumors. By selecting bacteriophage-derived promoters, a specific bacteriophage RNA polymerase gene, a membrane-disrupting protein gene, and a therapeutic protein gene, the expression vector is divided into a protein expression control module, a drug delivery module, and a drug-mediated killing module. By organically combining the three modules, a three-in-one system is formed, and a system and method for stable and efficient drug protein expression, drug delivery, and drug-mediated killing are realized.

The application belongs to the field of biology and discloses a plasmid containing Haemophilus parasuis OmpAm outer membrane protein gene and Mycoplasma hyopneumoniae membrane protein gene; the Mycoplasma hyopneumoniae membrane protein gene is one or more combinations of Mycoplasma hyopneumoniae membrane protein P46 gene, Mycoplasma hyopneumoniae membrane protein P65 gene and Mycoplasma hyopneumoniae membrane protein P159 gene. The plasmid contains OmpAm outer membrane protein gene and Mycoplasma hyopneumoniae membrane protein gene, target genes amplified from the vector by primers are used to prepare recombinant Haemophilus parasuis by natural transformation, and one or more of Mhp antigen proteins such as P46 protein, P65 protein and P159 protein can be effectively expressed, so that the effect of double immunization is achieved. Meanwhile, the application also provides recombinant Haemophilus parasuis and application thereof.

The invention discloses an influenza H5 subtype recombinant virus based on a VSV carrier, an inactivated vaccine and a preparation method of the influenza H5 subtype recombinant virus, and relates to the technical field of research and development of biological vaccines. The genome of the recombinant virus comprises a VSV genome without a surface envelope protein gene, and an NA gene and an HA gene of an H5 subtype avian influenza virus strain; the NA gene and the HA gene are located between an M gene and an L gene of a VSV genome, and the NA gene and the HA gene are connected in series in the 5 '-3' direction. The recombinant virus rescued by the reverse genetic technology can effectively express the NA antigen and the HA antigen of the H5 subtype avian influenza virus. The inactivated vaccine provided by the invention comprises the inactivated recombinant virus and a vaccine adjuvant, the inactivated recombinant virus loses replication ability, the NA protein and HA protein of the inactivated virus can still play an immune role under the assistance of the specific adjuvant, high-level avian influenza virus hemagglutination inhibition titer can be induced to be generated after a mouse is immunized, and the inactivated vaccine can be used for treating avian influenza virus hemagglutination. The method has the advantage of high safety.

The present invention discloses the application of the rice blast fungus membrane protein gene MoPH1 in regulating the pathogenicity of rice blast fungus, belonging to the field of biotechnology. In the present invention, a gene knockout vector is constructed and introduced into the protoplasts of rice blast fungus, and the gene is knocked out from rice blast fungus by homologous recombination to obtain the knockout mutant ΔMoph1. Experiments prove that compared with the wild type of rice blast fungus, the vegetative growth and development of the knockout mutant ΔMoph1 are slow, and the number of conidia is reduced, the function of appressoria is abnormal, and it is more tolerant to cell wall stress and osmotic stress, etc.; the pathogenicity test shows that the deletion of MoPH1 significantly reduces the pathogenicity of rice blast fungus; after the gene is complemented, its pathogenicity is restored to the wild type level. The present invention proves that MoPH1 is a pathogenic gene of rice blast fungus. Studying the membrane protein coding gene MoPH1 in rice blast fungus helps to deeply understand the pathogenic molecular mechanism of rice blast fungus and provides a target gene for the development of effective fungicides.

The invention discloses a Cronobacter sakazakii specific outer membrane protein gene target based on RPA-CRISPR, a primer, a detection system and a detection method. According to the present invention, the target sequence, the RPA primer and the crRNA have good inter-genus specificity, and the constructed detection method has no specific recognition reaction on other genus species, has a certain broad spectrum on other Cronobacter species of the same genus, and has accurate and reliable detection results. Besides, the detection method is combined with a boiling method or a kit to extract sample DNA, and on the premise that thallus enrichment is not carried out, the detection sensitivity of the cronobacter sakazakii genome DNA is 10 <-4 > ng / mu L, the target sensitivity is 1.43 copies / mu L, and the bacterial liquid sensitivity is 6 CFU / mL. The method is simple in operation, high in sensitivity and reliability and suitable for on-site rapid detection of cronobacter sakazakii in related supervision departments when standard detection is carried out on infant formula milk powder and goat milk powder, the sample detection limit reaches 100 CFU / mL, and the method is simple in sample pretreatment, convenient to operate, high in sensitivity and high in reliability and is suitable for on-site rapid detection of cronobacter sakazakii in related supervision departments.

The invention relates to a grass carp cell membrane protein gene CRISPR (clustered regularly interspaced short palindromic repeats) activated plasmid library. The library comprises CRISPR activated plasmids for targeted activation of 3498 candidate grass carp cell membrane protein genes; the invention further relates to a recombinant lentivirus capable of infecting fish cells and a packaging system of the recombinant lentivirus. The invention provides a CRISPR (clustered regularly interspaced short palindromic repeats) activation library aiming at related genes of grass carp cell membrane protein for the first time, and further provides recombinant lentivirus capable of infecting fish cells and a packaging system of the recombinant lentivirus. Protein on a grass carp cell membrane is identified through experiments, genes which exist in genome prediction but are not actually expressed can be excluded, and a library which accurately reflects related genes of grass carp cell membrane protein is formed. The CRISPR activation library provided by the invention avoids the situation of cell death after essential genes in a gene knockout library are knocked out, can comprehensively aim at essential or non-essential genes for cell survival, and can be widely applied to scenes such as virus receptor identification and / or gene function research and / or drug screening.

The application discloses a preparation method and application of a recombinant adenovirus spontaneously generating virus-like particles, and relates to the technical field of biology. The preparation method of the recombinant adenovirus comprises the following steps: connecting a virus membrane protein gene and a budding-promoting EARP gene to construct a recombinant exogenous virus membrane protein gene; inserting the recombinant exogenous virus membrane protein gene into an E3 region of an adenovirus skeleton plasmid to obtain a recombinant skeleton plasmid; inserting the recombinant exogenous virus membrane protein gene into an E1 region of an adenovirus shuttle plasmid to obtain a recombinant shuttle plasmid; and co-transfecting the recombinant skeleton plasmid and the recombinant shuttle plasmid into a host cell to obtain a recombinant adenovirus spontaneously generating virus-like particles (VLP). The recombinant adenovirus constructed by using the preparation method can spontaneously assemble VLPs containing virus outer membrane proteins in an in-vivo or in-vitro infection process, and thus the immunogenicity of a vaccine can be greatly improved, the VLP production process is simplified, and the production cost of the vaccine is reduced.

The invention relates to an application of a lysosome-associated membrane protein 3 gene in regulation and control of white spot syndrome virus resistance of a host. A nucleotide sequence for coding the gene of the lysosome-associated membrane protein 3 is shown as SEQ ID NO: 1. The ORF sequence of the lysosome related membrane protein 3 gene is 753bp in length, 250 amino acids are coded in total, and the gene can regulate the WSSV infection resistance of a host, is applied to prevention and treatment of WSSV infection as a potential target for gene editing animal design, and has great significance in reducing abuse of drugs.

The invention belongs to the technical field of molecular biological detection, and particularly relates to a product for LAMP-CRISPR detection of porcine epidemic diarrhea virus, which comprises an LAMP primer group designed based on highly conservative membrane protein (M) gene in porcine epidemic diarrhea virus genome and a group of sgRNA. According to the product disclosed by the invention, an LAMP amplification system and CRISPR / Cas12b detection can be fused in one-tube constant-temperature reaction, only one reaction temperature needs to be set, a cover does not need to be opened in the whole process, and the operation is simple, convenient and efficient. And the product has high sensitivity and excellent specificity, effectively eliminates false positive interference, and is more suitable for clinical rapid and accurate detection. Meanwhile, the product disclosed by the invention can also be combined with an immune colloidal gold test strip to be better applied clinically, so that the detection of the porcine epidemic diarrhea virus does not depend on large-scale experimental equipment, and the obvious advantages of high efficiency and convenience are achieved.

The invention discloses an application of an unknown functional domain 679 membrane protein gene SpDUF679 of stigma leptopodum in drought stress tolerance of plants, and belongs to the technical field of gene engineering. The CDS sequence of the unknown functional domain 679 membrane protein gene SpDUF679 is as shown in SEQ ID NO.5, and the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO.6. The invention further discloses a preparation method of the unknown functional domain 679 membrane protein gene SpDUF679. According to the invention, a new unknown functional domain membrane protein 679 of stigma leptostachys is found and named as SpDUF679, meanwhile, a plant expression vector of the gene is successfully constructed and transformed, and the gene is verified to have an important function of enhancing the drought stress resistance of plants in transgenic plants. The invention is of great significance to reveal the stress resistance mechanism of stigma leptostachyum, enrich the plant stress resistance molecular biology theory and improve the stress resistance of plants.

The present invention relates to a method for modifying the envelope of lentivirus to improve the transduction efficiency of NK cells, belonging to the technical field of cell transduction, and specifically comprising the following steps: S1. Using bioinformatics technology to perform directional modification on the amino acid sequence of the key binding site between the envelope protein and the receptor to obtain a modified envelope protein gene; S2. Optimizing the coding sequence of the modified envelope protein according to human codon preference to obtain an optimized envelope protein gene; inserting the optimized envelope protein gene into a lentiviral vector to construct a lentiviral packaging system for infecting cell lines. The present invention uses bioinformatics technology to change the coding sequence of the BaEV glycoprotein to replace the VSVG protein of the envelope protein on the surface of the lentivirus, and combines codon optimization and plasmid construction technology to change the nucleic acid sequence of the virus into a sequence more suitable for expression in human cells, ensuring that the modified viral vector has both high infection efficiency and safety.

The present disclosure relates to systems and methods for improved breeding of elite octoploid strawberry varieties. Described herein are haploid-inducing strawberry plants, plant parts, and plant lines comprising one or more genetic modifications resulting in decreased expression of one or more DMP (Domain of Unknown Function 679 Membrane Protein) genes. Also described herein are methods of using thereof to generate true homozygous octoploid strawberry lines, as well as true breeding Fl hybrid octoploid strawberry seeds produced by crossing two true homozygous octoploid strawberry plants. Further described herein are methods and compositions for producing haploid-inducing strawberry plants, including methods for introducing genetic modifications resulting in decreased expression of one or more DMP genes.