The invention belongs to the technical field of spectrum detection systems and in particular relates to a fluorescence-related spectrum measurement system. Exciting light is limited within a flying volume by utilizing a tapered optical fiber, and meanwhile, a fluorescence signal is collected by utilizing an optical fiber, so that time-related photo counting is performed, and furthermore, a fluorescence-related spectrum is acquired. By using the method, a fluorescence confocal microscope, a total internal reflection fluorescence microscope or a two-photon fluorescence microscope is prevented from being used, so that the measurement cost of a fluorescence-related spectrum is reduced; meanwhile, the optical fiber can be penetrated into a solution, a reactor, a microfluidic chip, a cell or a body, so that space imitation of imaging equipment is eliminated; and the fluorescence-related spectrum measurement system can be widely applied to the aspects such as reaction dynamic research, livingcell analysis, biological detection, disease diagnosis and drug screening in the fields of life sciences, physics and chemicals.