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Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof

A technology for Bacillus subtilis and cucumber downy mildew, which is applied in the field of agricultural microorganisms, can solve the problems of pathogenic bacteria that are prone to drug resistance, cumbersome extraction process, and high production costs, and achieve the effects of not easy to produce drug resistance, no environmental pollution, and low cost

Active Publication Date: 2013-06-19
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction process of plant extracts is cumbersome, the production cost is high, and the pathogenic bacteria are prone to drug resistance after the use of artificially synthesized single substances, making this method difficult to apply

Method used

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  • Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof
  • Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof
  • Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of HMB20428 microbial bacterial agent is carried out according to the following steps:

[0046] (1) Strain activation: The strain HMB20428 (Bacillus subtilis) strain HMB20428 (Bacillus subtilis) strain HMB20428 stored at -40°C has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on December 20, 2011, and the preservation number is CGMCC No.5616) is activated at 30°C on LB plate medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), Pick a single colony and culture it at 30°C for 24 to 36 hours on LB slant medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), Activated strains;

[0047] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract...

Embodiment 2

[0050] Example 2 Comparative Test of Bacillus subtilis HMB20428 on the Control Effect of Cucumber Downy Mildew

[0051] (1) Test drug:

[0052] (1) Microbial agent: the liquid preparation of HMB20428 prepared in Example 1; diluted 50 times with water.

[0053] (2) Chemical agent: azoxystrobin suspension concentrate (250 g / L) (Syngenta Co., Ltd., UK), diluted 1000 times with water.

[0054] (3) Blank control: clear water

[0055] (2) Test method: This test was carried out in the laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The cucumber downy mildew used in this test came from the Pesticide Application Technology Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in 2010, and was formulated into sporangia (1 × 10 5 -1.5×10 5 individual / mL) suspension.

[0056] Get disease-free complete cucumber leaves, which are respectively immersed in: (1) in the 50-fold dilution of th...

Embodiment 3

[0061] Example 3 Comparative Test of Bacillus subtilis HMB20428 on the Control Effect of Cucumber Downy Mildew

[0062] (1) Test drug:

[0063] (1) Microbial agent: the HMB20428 liquid preparation prepared in Example 1, diluted 50 times with water;

[0064] (2) Chemical agent: silver Farley suspension concentrate (687.5 g / liter) (Germany Bayer Crop Science Company), diluted 800 times with water;

[0065] (3) Blank control: clear water.

[0066] (2) The time and place of the experiment: in December 2010, it was carried out in the artificial climate chamber of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0067] (3) Test method: screen plump cucumber seeds, sow them in plastic flower pots with a diameter of 20 cm when they are accelerated to the point of dew, 15 pots per treatment (2 plants per pot), repeat 3 times. Arranged in random groups. Take cucumber seedlings with 3-4 true leaves and consistent growth vigor, and spray the liq...

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Abstract

The invention discloses a Bacillus subtilis strain HMB20428 for preventing and treating cucumber ampho disease, and the Bacillus subtilis strain HMB20428 is preserved in the China General Microbiological Culture Collection Center on December 20, 2011, and the preserving registration number is CGMCC No.5616. The invention further discloses a microbial agent prepared by the strain and a preparationmethod thereof. The active ingredients of the microbial agent are Bacillus subtilis HMB20428 thalli and extracellular metabolite of the Bacillus subtilis HMB20428 thalli. The strain HMB20428 has strong specificity for the cucumber ampho disease; the control effect is high; and the average control effect can reach above 85%; through the method provided by the invention for preventing and treating the cucumber ampho disease, drug resistance is difficult to generate; the microbial agent is safe to human and livestock, and is free of environment pollution; and the preparation method is simple, the cost is low, and the microbial agent is convenient to use.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a bacillus subtilis for preventing and treating cucumber downy mildew, a microbial inoculum containing the bacillus subtilis, and their application in preventing and treating cucumber downy mildew. Background technique [0002] Cucumber downy mildew is an infectious airborne disease caused by Pseudoperonospora cubensis Rostow, which poses a huge threat to cucumber production. The methods of controlling cucumber downy mildew mainly include disease-resistant breeding, chemical control and ecological control (such as high-temperature stuffy shed, etc.). Because cucumber downy mildew is a quantitative trait genetically controlled by multiple genes, it is difficult to carry out disease-resistant breeding and the resistance is easy to lose; chemical control has the problems of easily producing drug resistance and polluting people and the environment; and the high-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 李社增马平鹿秀云郭庆港李宝庆
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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