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Yeast cell active metabolite capable of promoting growth and anti-stress capability of animals and preparation method thereof

A technology for animal growth and anti-stress, applied in the field of feed additives, can solve problems such as reduced productivity and weakened immunity, and achieve the effects of increasing utilization, promoting animal growth, and improving animal anti-stress ability

Active Publication Date: 2014-09-17
CHANGCHUN BORUI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of reduced productivity and weakened immunity caused by stress in animal production, the object of the present invention is to provide a yeast cell active metabolite that promotes animal growth and stress resistance and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An active yeast cell metabolite that promotes animal growth and anti-stress, the specific steps are as follows:

[0024] (1) Use molasses with 5% sugar content as the initial fermentation medium, inoculate Saccharomyces cerevisiae, and after culturing at 30°C for 8 hours, carry out uniform flow feeding, the sugar concentration of the feeding medium is 20%, and the feeding opening is 4s / 60s; when the dry weight of Saccharomyces cerevisiae is more than 30g / L, anaerobic fermentation is carried out.

[0025] (2) Unfed anaerobic fermentation was carried out for 24h in extreme environment to obtain extracellular metabolites.

[0026] The anaerobic fermentation conditions were set at 37±2°C, pH 10±0.5 (by 10% H 2 SO 4 and 2mol / L NaOH to adjust the pH), add NaCl 150±10mM, and introduce nitrogen for strict anaerobic fermentation for 24h.

[0027] (3) After the anaerobic fermentation, adjust the pH of the fermentation broth to 5-6, add papain at 0.2-2% of the dry weight of ye...

Embodiment 2

[0029] An active yeast cell metabolite that promotes animal growth and anti-stress, the specific steps are as follows:

[0030] (1) Use molasses with 5% sugar content as the initial fermentation medium, inoculate Saccharomyces cerevisiae, and after culturing at 30°C for 8 hours, carry out uniform flow feeding, the sugar concentration of the feeding medium is 20%, and the feeding opening is 4s / 60s; when the dry weight of Saccharomyces cerevisiae is more than 30g / L, anaerobic fermentation is carried out.

[0031] (2) Unfed anaerobic fermentation was carried out for 24h in extreme environment to obtain extracellular metabolites.

[0032] The anaerobic fermentation conditions were set at 37±2°C, pH 3±0.5 (by 10% H 2 SO 4 and 2mol / L NaOH to adjust the pH), add NaCl 150±10mM, and introduce nitrogen for strict anaerobic fermentation for 24h.

[0033] (3) After the anaerobic fermentation, adjust the pH of the fermentation broth to 5-6, add papain at 0.2-2% of the dry weight of yea...

Embodiment 3

[0035] An active yeast cell metabolite that promotes animal growth and anti-stress, the specific steps are as follows:

[0036] (1) Use molasses with 5% sugar content as the initial fermentation medium, inoculate Saccharomyces cerevisiae, and after culturing at 30°C for 8 hours, carry out uniform flow feeding, the sugar concentration of the feeding medium is 20%, and the feeding opening is 4s / 60s; when the dry weight of Saccharomyces cerevisiae is more than 30g / L, anaerobic fermentation is carried out.

[0037] (2) Unfed anaerobic fermentation was carried out for 24h in extreme environment to obtain extracellular metabolites.

[0038] The anaerobic fermentation conditions were set at 10±2°C, pH 8±0.5 (by 10% H 2 SO 4 and 2mol / L NaOH to adjust the pH), add NaCl 150±10mM, and introduce nitrogen for strict anaerobic fermentation for 24h.

[0039] (3) After the anaerobic fermentation, adjust the pH of the fermentation broth to 5-6, add papain at 0.2-2% of the dry weight of yea...

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PUM

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Abstract

The invention discloses a yeast cell active metabolite capable of promoting the growth and anti-stress capability of animals and a preparation method thereof. The preparation method of the yeast cell active metabolite capable of promoting the growth and anti-stress capability of animals comprises the following steps of firstly obtaining a fermentation broth of which the yeast dry weight is greater than 30g / L through aerobic fermentation; carrying out anaerobic fermentation for 4-24 hours in environment with an extreme temperature, an extreme pH value or an extreme osmotic pressure to obtain an extracellular metabolite; breaking the wall of the yeast in an autolyzing manner by addition of protease, releasing an intracellular metabolite; centrifuging, filtering, separating and purifying the active metabolite product of which the molecular weight is less than 30,000 daltons in the supernatant. The yeast cell active metabolite prepared by the invention can be used as an feed additive for feeding animals, has the effects of improving the morphology of intestinal mucosa of animals, maintaining the balance of intestinal flora, increasing the utilization rate of the feed, promoting the growth of animals and improving the anti-stress capacity of animals and has the characteristics of safe feeding, small addition and no residue.

Description

technical field [0001] The invention relates to a feed additive, in particular to a yeast cell active metabolite that promotes animal growth and resists stress and a preparation method thereof. Background technique [0002] The stress response of animals is an adaptive response of the animal body when it is stimulated by changes in the internal and external environment. The stress response is stimulated by a variety of factors, including internal genetic factors, production and reproduction factors, external environmental factors, etc. These stress factors "stressors" stimulate animals will cause harm to the animal body, which is manifested as animal spirits Fatigue, loss of appetite, or extreme excitement, mania, reduced feed intake; weakened anabolism, reduced feed conversion rate, reduced production performance, weakened immunity, allergies and other clinical symptoms. [0003] As the earliest known microorganism, yeast is widely used in food, cosmetics, medicine, condim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02A23K1/16C12R1/865C12R1/84C12R1/72A23K10/12
Inventor 甄玉国冀红芹张学峰赵小丽郑艳秋关艳玲赵巍
Owner CHANGCHUN BORUI FEED
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