5 results about "Cell metabolite" patented technology

The application belongs to the technical field of single-cell metabolite detection, and specifically provides a single-cell metabolite qualitative method and system, the qualitative method comprising the following steps: A1. obtaining raw data of metabolites by using a detection module; A2. calculating similarity scores of the raw data and candidate data in a database by using a data analysis module; A3. arranging the candidate data in descending order from high to low according to the similarity scores, and cutting off the top 20% of candidate data with high confidence to form sequence 1, denoted as [candidate metabolites] i ; A4. re-arranging the candidate data in sequence 1 according to the biological rationality of metabolites by using a data arrangement module to form sequence 2, denoted as [candidate metabolites] j ; A5. qualitatively determining the metabolites as the candidate metabolites equal to min(i+j) . The application has the advantages of high metabolite qualitative accuracy, high efficiency, strong applicability and the like.

The application discloses a kind of single-cell metabolite in vivo in situ detection method, which comprises the following steps: establishing single-cell cytoplasm sampling volume / single-cell volume relative quantification method, obtaining the relative maximum cytoplasm extraction volume when maintaining single-cell survival;Build composite ion source, obtain the minimum sampling volume of 150 metabolites detected from single cytoplasm, obtain the sampling volume of single-cell cytoplasm in the in vivo in situ detection of metabolome;Through picoliter pump probe microscope fixed-point positioning puncture cell membrane, extract the cell cytoplasm of the above sampling volume;Metabolites in the extracted single-cell cytoplasm are ionized using composite ion source;Metabolites after ionization are detected by mass spectrometry.The application can realize multiple single-cell cytoplasm extraction of in vivo embryo during development, mass spectrometry metabolite detection, while ensuring cell survival, multiple sampling, while realizing accurate detection of trace metabolites in single cell.

The invention discloses a single-cell multi-dimensional mass spectrum data processing method, and relates to the technical field of data processing. The method comprises the steps that at least two original secondary spectrograms are acquired, the secondary spectrograms comprise ion mobility mass spectrum data frames of at least two continuous secondary spectrogram acquisition windows, the ion mobility mass spectrum data frames comprise mass-to-charge ratios and ion mobility values of target objects, and the target objects comprise metabolite ions and single cell marker ions; carrying out single cell ionization event identification processing on each original secondary spectrogram, and screening candidate secondary spectrograms from at least two original secondary spectrograms; determining a marker ion mobility mass spectrum data frame of the single cell marker ion corresponding to each secondary spectrogram acquisition window where the metabolite ion is located according to the acquisition mode of the original secondary spectrogram and the candidate secondary spectrogram; and summing the secondary spectrograms corresponding to the marker ion mobility mass spectrum data frames of the metabolite ions to obtain a target secondary spectrogram of the metabolite ions, so that the identification accuracy of the single cell metabolite can be improved.

The invention discloses a method for evaluating, regulating and controlling ultraviolet B-band (ultraviolet B, UV-B) irradiation enrichment of grape secondary metabolites on the basis of a microtubulin array. The material is used for evaluating a microtubulin array and preparing a sample solution for determining secondary metabolites by applying a high performance liquid chromatography, and by detecting the enrichment degrees of the cell metabolites of rutin, kaempferol, myricetin and resveratrol before and after irradiation, the microtubulin array can be used for evaluating the microtubulin array and preparing the sample solution for determining the secondary metabolites. The raw materials for preparing food and cosmetics with anti-inflammatory and anti-oxidation effects are obtained. The invention creates a complete method from treatment time and treatment steps of UV-B irradiation of the American sandy grape cell suspension, monitoring of dynamic changes of tubulin and cell morphology indexes, and detection of changes of cell metabolites such as rutin, kaempferol, myricetin and resveratrol before and after irradiation. And a new way is provided for applying the enriched grape natural secondary metabolites to production of food and cosmetics with anti-inflammatory and anti-oxidation effects.

The invention relates to a real-time monitoring method for cell metabolites, and belongs to the technical field of biosensing and cell analysis. The method comprises the following steps: culturing cells on a substrate integrated with a first (electrochemical) type (optical) sensing unit and a second (optical) type (electrochemical) sensing unit which are arranged in a coplanar and staggered manner; in the monitoring process, electric signals of all the first-class sensing units and optical images of all the second-class sensing units are synchronously collected through hardware time sequence control, and timestamps and position information are recorded; performing fusion processing on the signals in the same period, namely generating a first spatial distribution data frame according to the position information combination electric signal, converting the optical image to generate a second spatial distribution data frame, and aligning the first spatial distribution data frame and the second spatial distribution data frame according to a pre-calibrated spatial mapping relation; and finally, based on the aligned data frame pairs on the time sequence, generating dynamic association information reflecting the time-space collaborative change of the concentrations of the at least two metabolites. According to the method, multi-parameter and high-temporal-spatial-resolution in-situ real-time monitoring and visual analysis of the metabolic activity of the living cells are realized.