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Preparation method of live chlorella suspension capable of improving human immunity

A technology of chlorella and suspension, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, food ingredients containing gas, etc., can solve the problems of waste of chlorella cell resources, etc., and achieve the improvement of human immunity. Absorption, easy absorption effect

Inactive Publication Date: 2018-09-07
GANSU DEFU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method clarifies the suspension and removes the formed sediment, which still results in a waste of resources for Chlorella cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method for improving the fresh chlorella suspension of human body immunity, comprises the steps:

[0032] 1) Prepare culture medium

[0033] ①Preparation of culture medium raw materials: ammonium nitrate 0.2-0.4g, calcium superphosphate solution 0.3-0.5ml, ferric chloride solution 0.15ml, cobalt nitrate solution 0.1ml, copper sulfate solution 0.1ml, carbonic acid solution 5-10ml, tap water 1000ml, Wherein, the mass concentrations of the calcium superphosphate, ferric chloride, cobalt nitrate and copper sulfate solutions are 10%, 1%, 0.1% and 0.1% respectively; the pH value of the carbonic acid solution is 3.0-5.0;

[0034] ②Preparation of culture medium: add the raw material of the culture solution into the chlorella culture device, mix well and evenly, and raise the temperature to 28-32°C under slow stirring to obtain the culture medium;

[0035] 2) Initial culture

[0036] Insert the planktonic strain of Chlorella into the culture medium, so that the i...

Embodiment 2

[0042] A preparation method for improving the fresh chlorella suspension of human body immunity, comprises the steps:

[0043] 1) Prepare culture medium

[0044] ①Preparation of culture medium raw materials: ammonium nitrate 0.2-0.4g, calcium superphosphate solution 0.3-0.5ml, ferric chloride solution 0.15ml, cobalt nitrate solution 0.1ml, copper sulfate solution 0.1ml, carbonic acid solution 5-10ml, tap water 1000ml, Wherein, the mass concentrations of the calcium superphosphate, ferric chloride, cobalt nitrate and copper sulfate solutions are 10%, 1%, 0.1% and 0.1% respectively; the pH value of the carbonic acid solution is 3.0-5.0;

[0045] ②Preparation of culture medium: add the raw material of the culture solution into the chlorella culture device, mix well and evenly, and raise the temperature to 28-32°C under slow stirring to obtain the culture medium;

[0046] 2) Preparation of concentrated chlorella suspension

[0047] Insert the planktonic strain of Chlorella into ...

Embodiment 3

[0049] A preparation method for improving the fresh chlorella suspension of human body immunity, comprises the steps:

[0050] 1) Prepare culture medium

[0051] ①Preparation of culture medium raw materials: ammonium nitrate 0.2-0.4g, calcium superphosphate solution 0.3-0.5ml, ferric chloride solution 0.15ml, cobalt nitrate solution 0.1ml, copper sulfate solution 0.1ml, carbonic acid solution 5-10ml, tap water 1000ml, Wherein, the mass concentrations of the calcium superphosphate, ferric chloride, cobalt nitrate and copper sulfate solutions are 10%, 1%, 0.1% and 0.1% respectively; the pH value of the carbonic acid solution is 3.0-5.0;

[0052] ②Preparation of culture medium: add the raw material of the culture solution into the chlorella culture device, mix well and evenly, and raise the temperature to 28-32°C under slow stirring to obtain the culture medium;

[0053] 2) Initial culture

[0054] Insert the planktonic strain of Chlorella into the culture medium, so that the i...

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Abstract

The invention discloses a preparation method of a live chlorella suspension capable of improving human immunity. The method comprises the following steps: 1) preparing a culture medium; 2) performinginitial culture: inoculating a strain suspension into the culture medium and culturing the strain suspension for 4 d continuously to obtain an initial chlorella suspension; 3) preparing concentrated chlorella suspension: (1) preparing concentrated chlorella suspension cell biomass; (2) preparing a concentrated chlorella culture solution; (3) preparing the concentrated chlorella suspension: preparing the concentrated chlorella suspension from the concentrated chlorella suspension cell biomass and the concentrated chlorella culture solution by mixing. The mixed suspension based on live cells andextracellular metabolites of a chlorella strain is formed by effectively using the extracellular metabolites decomposed into the culture solution, and the suspension reserves all bioactive substancesof chlorella, is easily absorbed by a human body, has the advantages of long storage period and the like and particularly has the functions of improving human immunity and preventing diseases.

Description

technical field [0001] The invention belongs to the technical field of health product production, and in particular relates to a preparation method of fresh chlorella suspension for improving human immunity. Background technique [0002] Chlorella is a general unicellular green algae belonging to the Chlorella phylum Chlorella. It is a spherical single-cell freshwater algae with a diameter of 3-8μm. It is one of the earliest life on earth. It appeared more than 2 billion years ago. It has never changed, and it is a highly efficient photosynthetic plant that grows and reproduces with photosynthetic autotrophy and is widely distributed. It is rich in protein, vitamins, minerals, dietary fiber, nucleic acid and chlorophyll, etc., and is an indispensable nutrient for maintaining and promoting human health. In particular, it contains eye-catching biologically active substances such as glycoproteins, polysaccharides, and up to 13% nucleic acids, which can enhance human immunity, ...

Claims

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Application Information

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IPC IPC(8): C12N1/12A23L2/38A23L2/54A23L2/44A23L33/00C12R1/89
CPCA23L2/38A23L2/44A23L2/54A23L33/00C12N1/12A23V2002/00A23V2200/324A23V2250/11
Inventor 常贵包苏宁张国威
Owner GANSU DEFU BIOTECH
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