Bacillus pumilus for controlling cottonbollblight and microorganism bacterium agent thereof
A technology of Bacillus pumilus and cotton boll blight, which is applied in the field of prevention and control of cotton boll blight, can solve the problems of few researches on the biological control of cotton boll blight, and achieve the effects of less drug resistance, strong pertinence, and high control efficiency
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Embodiment 1
[0044] The preparation of embodiment 1HMB22277 microbial bacterial agent
[0045] Proceed as follows:
[0046] (1) Strain activation: Bacillus pumilus (Bacillus pumilus) strain HMB22277 stored at -40°C (preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on December 20, 2011, the preservation number is CGMCC No. .5615) was activated at 30°C on LB plate medium (its composition and weight ratio were: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), and single Bacterial colonies were cultured at 30°C for 24 hours on LB slant medium (its composition and weight ratio were: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) to obtain activated strains;
[0047] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 1000mL), in a...
Embodiment 2
[0050] Example 2 Antagonism Test of Bacillus pumilus HMB22277 to Phytophthora cotton boll Phytophthora
[0051] (1) Test time and place: In early August 2010, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.
[0052] (2) Test method:
[0053] (1) Source of cotton boll Phytophthora bacterium: Phytophthora 5-1 was collected from cotton bolls in Hejie Village, Hejie Town, Xianxian County, Cangzhou City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Hebei Agricultural University The Plant Pathology Department of the Institute of Plant Protection identified it as Phytophthora boehmeriae, and the pathogenicity test showed strong pathogenicity.
[0054] (2) Tablet confrontation experiment:
[0055] First, Phytophthora 5-1 is activated on the PDA plate, and after 5 days of cultivation,...
Embodiment 3
[0059] Example 3 Comparative Test of Bacillus pumilus HMB22277 on the Control Effect of Cotton Boll Blight
[0060] (1) Test drug:
[0061] (1) Microbial agent: the HMB22277 liquid preparation prepared in Example 1 was diluted 50 times with water.
[0062] (2) Chemical agent: aluminum triethylphosphonate wettable powder (80%) (Zhejiang Jiahua Chemical Co., Ltd.), diluted 800 times with water.
[0063] (3) Blank control: clear water.
[0064] (2) The time and place of the experiment: in late August 2010, it was carried out in the artificial climate chamber of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.
[0065] (3) Test method: collect healthy bolls from the middle and upper parts of cotton plants in the test field (about 30 days after boll formation), require fresh bract leaves, and disinfect the surface with 75% alcohol after bringing them back to the laboratory, and let them dry naturally. Spraying on the surface of cotton bolls:...
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