Bacillus pumilus for controlling cottonbollblight and microorganism bacterium agent thereof

A technology of Bacillus pumilus and cotton boll blight, which is applied in the field of prevention and control of cotton boll blight, can solve the problems of few researches on the biological control of cotton boll blight, and achieve the effects of less drug resistance, strong pertinence, and high control efficiency

Active Publication Date: 2013-06-19
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few studies on the biological control of cotton boll blight

Method used

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  • Bacillus pumilus for controlling cottonbollblight and microorganism bacterium agent thereof
  • Bacillus pumilus for controlling cottonbollblight and microorganism bacterium agent thereof
  • Bacillus pumilus for controlling cottonbollblight and microorganism bacterium agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The preparation of embodiment 1HMB22277 microbial bacterial agent

[0045] Proceed as follows:

[0046] (1) Strain activation: Bacillus pumilus (Bacillus pumilus) strain HMB22277 stored at -40°C (preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures on December 20, 2011, the preservation number is CGMCC No. .5615) was activated at 30°C on LB plate medium (its composition and weight ratio were: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL), and single Bacterial colonies were cultured at 30°C for 24 hours on LB slant medium (its composition and weight ratio were: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) to obtain activated strains;

[0047] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, water 1000mL), in a...

Embodiment 2

[0050] Example 2 Antagonism Test of Bacillus pumilus HMB22277 to Phytophthora cotton boll Phytophthora

[0051] (1) Test time and place: In early August 2010, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0052] (2) Test method:

[0053] (1) Source of cotton boll Phytophthora bacterium: Phytophthora 5-1 was collected from cotton bolls in Hejie Village, Hejie Town, Xianxian County, Cangzhou City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, Hebei Agricultural University The Plant Pathology Department of the Institute of Plant Protection identified it as Phytophthora boehmeriae, and the pathogenicity test showed strong pathogenicity.

[0054] (2) Tablet confrontation experiment:

[0055] First, Phytophthora 5-1 is activated on the PDA plate, and after 5 days of cultivation,...

Embodiment 3

[0059] Example 3 Comparative Test of Bacillus pumilus HMB22277 on the Control Effect of Cotton Boll Blight

[0060] (1) Test drug:

[0061] (1) Microbial agent: the HMB22277 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0062] (2) Chemical agent: aluminum triethylphosphonate wettable powder (80%) (Zhejiang Jiahua Chemical Co., Ltd.), diluted 800 times with water.

[0063] (3) Blank control: clear water.

[0064] (2) The time and place of the experiment: in late August 2010, it was carried out in the artificial climate chamber of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0065] (3) Test method: collect healthy bolls from the middle and upper parts of cotton plants in the test field (about 30 days after boll formation), require fresh bract leaves, and disinfect the surface with 75% alcohol after bringing them back to the laboratory, and let them dry naturally. Spraying on the surface of cotton bolls:...

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Abstract

The invention discloses a Bacillus pumilus strain HMB22277 for controlling cottonbollblight which is preserved in the China General Microbiological Culture Collection Center on December 20, 2011 with the preservation number of CGMCC NO. 5615. The invention further discloses a microorganism bacterium agent produced by using the microbe and a preparation method thereof. The active component of the microorganism bacterium agent is Bacillus pumilus HMB22277 thalli and its extracellular metabolite. According to the invention, the Bacillus pumilus HMB22277 has strong targeted performance on cottonbollblight with high control effect, wherein the average control effect is higher than 77%; secondly the microorganism bacterium agent of the invention is safe for human and animals and has no environmental pollution and doesn't easily develop drug resistance; in addition in addition, the preparation method of the microorganism bacterium agent is simple, the cost is low and the application is convenient.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a bacillus pumilus for preventing and treating cotton boll blight, a microbial agent containing the bacillus pumilus, and their application in preventing and treating cotton boll blight. Background technique [0002] Cotton boll blight is a serious cotton disease caused by Phytophthora boehmeriae sawada. It not only directly damages cotton bolls, but also provides infection conditions for other pathogens. The methods to prevent and control cotton boll blight are mainly cultivation measures such as pushing plants and ridges, picking rotten bolls early, and chemical agent control. At present, the economic benefits of growing cotton in my country are relatively low, and the labor costs are high. Farmers are unwilling to invest too much energy in pushing the plants together; picking rotten bolls early can only keep part of the yield, which is not enough to preve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 李宝庆马平李社增鹿秀云郭庆港
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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