Microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and preparation method of microecological preparation

A technology of probiotics and japonicus imitation, applied to medical preparations containing active ingredients, pharmaceutical formulas, antibacterial drugs, etc., can solve problems such as drug residues, deterioration of breeding environment, and enhanced drug resistance of pathogens, and achieve cost reduction. Low cost, easy to use, high anti-efficiency effect

Active Publication Date: 2015-02-25
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to control the occurrence of diseases of japonicus japonicus, many farmers choose to use a large amount of antibiotics, and long-term use

Method used

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  • Microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and preparation method of microecological preparation
  • Microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and preparation method of microecological preparation
  • Microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and preparation method of microecological preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A microbial preparation produced by bacillus megaterium H1, the active ingredient of which is the thalline of bacillus megaterium H1 and its extracellular metabolites. Its preparation method is carried out according to the following steps:

[0036] (1) Strain activation: Streak and activate the Bacillus megaterium H1 strain stored at -80°C on tryptone soy agar medium, culture at 28°C until a single colony grows, and then pick a single colony and inoculate it on tryptone soy On the slant medium of agar medium, culture at 28°C for 24 hours to obtain the activated strain.

[0037] (2) Seed solution preparation: prepare tryptone soybean broth liquid medium according to conventional methods, its composition and composition: tryptone 1.5% (g / 100ml), soybean peptone 0.5% (g / 100ml), sodium chloride 0.5% (g / 100ml); In a 500mL Erlenmeyer flask, 200mL of tryptone soybean broth liquid medium was filled, and the pH was 7.6. Put it in a high-pressure steam sterilizer, and sterilize...

Embodiment 2

[0041] From January to March 2012, we conducted a 2-month experiment on the cultivation of imitation japonicus seedlings in Shandong Anyuan Aquatic Products Co., Ltd. The probiotics of Bacillus megaterium H1 are produced in a fermenter according to the method of Example 1, and the number of live bacteria reaches 3.5×10 10 CFU / ml, the bacterial solution is light brown and stored at room temperature. The experiment was carried out in 6 imitation japonicus seedling pools in the company's experimental workshop. A control group and an experimental group sprayed with Bacillus megaterium H1 microecological preparations were set up. Each group had 3 parallels, and each pool had a water volume of 8 cubic meters. Select healthy juvenile ginseng individuals (average body weight is 0.81±0.37g, body length 1.3-2.1cm), and the density of individuals is 0.5kg / square water body for 24 hours aerated culture. Ensure that the dissolved oxygen in the water body is >5mg / L, the water temperature i...

Embodiment 3

[0043] In December 2013, we conducted a 3-month experiment on overwintering cultivation of imitation japonicus seedlings in Qingdao Beibao Ocean Technology Co., Ltd. Bacillus megaterium strain H1 is produced by the company according to the method of embodiment 1 in the secondary fermenter, and the number of bacteria reaches 8.6×10 10 CFU / ml, stored at room temperature. The experiment was carried out in 9 imitation sea cucumber seedling pools in the circulating water workshop of the company. A control group, an experimental group fed with Bacillus megaterium H1 microecological preparations and an experimental group fed with Bacillus subtilis liquid were set up. sale (nominal 10 11 CFU / ml), each group of 3 parallel, wherein each pool of water is 6 cubic meters. Healthy juvenile ginseng individuals (average body weight 3.12±0.97g, body length 3.01-4.12cm) were selected, and the individual stocking density was 0.5kg / square water body. The breeding pond is inflated for 24 hours,...

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Abstract

The invention relates to a microecological preparation used for preventing and treating apostichopus japonicus putrid skin syndrome and a preparation method of the microecological preparation and belongs to the field of aquatic microorganisms. Active ingredients of the microecological preparation are thalli of bacillus megatherium H1 and extracellular metabolites of the bacillus megatherium H1; the bacillus megatherium strain is separated from intestinal tract excrement of apostichopus japonicus which is healthily cultivated in Penglai, Shandong, detection of safety, bacteriostatic activity and toxicity and artificial antagonistic vibrio splendidus experiments verify that the bacillus megatherium H1 is antagonistic to vibrio splendidus, young apostichopus japonicus is effectively protected, the bacillus megatherium H1 can be used for preventing and treating apostichopus japonicus 'putrid skin syndrome' caused by vibrio splendidus and survival rate of an apostichopus japonicus seedling protecting stage is increased.

Description

Technical field: [0001] The invention belongs to the field of aquatic microorganisms, and in particular relates to a micro-ecological preparation and a preparation method for preventing and treating "rot skin syndrome" of sea cucumbers. Background technique: [0002] Apostichopus japonicas Selenka (Apostichopus japonicas Selenka) farming has become one of the important pillars of my country's aquaculture industry because of its highest output value of a single species and an annual growth rate of 20%. In 2011, the breeding area of ​​japonicus in Shandong Province reached 53,000 hectares, with an annual output of 71,000 tons (more than half of the national total output), and an annual output value of 16 billion yuan. However, with the continuous expansion of sea cucumber breeding scale in recent years, various diseases have come one after another, seriously restricting the healthy and sustainable development of the industry. The "rotting skin syndrome" that occurs in the see...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61P31/04
CPCA61K35/742C12N1/20
Inventor 祁自忠韩茵陈四清邹安革王亮余彬
Owner OCEAN UNIV OF CHINA
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