10 results about "Vibrio splendidus" patented technology

The application belongs to the technical field of molecular biology, and particularly relates to a long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein and application. The amino acid sequence of the long oyster C-type lectin CgCLec-TM2 functional domain CRD recombinant protein is shown as SEQ ID NO. 1. The rCRD obtained by the application has binding capacity to various pathogen-associated molecular patterns (PAMPs), can bind and agglutinate various microorganisms, has a growth inhibiting effect on gram-negative bacteria Vibrio splendidus and Escherichia coli, and can be used for the development of bacteriostatic feed additives and new-type immune enhancers.

The invention provides an antibacterial peptide ATMP23 mutant with improved antibacterial activity and application of the antibacterial peptide ATMP23 mutant in preparation of an antibacterial agent. The amino acid sequence of the antibacterial peptide ATMP23 provided by the invention is shown as SEQ ID NO: 1, amino acid mutation and screening are carried out on the basis of the amino acid sequence, and antibacterial peptide mutants A23-M4, A23-M5 and A23-M6 with significantly improved antibacterial activity are obtained. The antibacterial peptide ATMP23 mutant provided by the invention is small in molecular weight, convenient to artificially synthesize and low in cost, and has a good bactericidal effect on clostridium welchii, staphylococcus aureus, streptococcus, vibrio parahaemolyticus, vibrio alginolyticus, vibrio splendidus, salmonella, escherichia coli and the like; when being applied to livestock and aquatic feed, the feed conversion ratio of livestock and aquatic products can be remarkably reduced, the growth performance and immune function indexes of bred animals can be improved, and the feed additive can be widely applied to the industries of feed, food, medicine and the like.

The application discloses a Pseudomonas australis 94-6Y strain, a bacterial agent and application thereof, and belongs to the technical field of microorganisms. Fictibacillus macauensis The Pseudomonas australis 94-6Y strain is classified and named as Pseudomonas australis, the strain number is 94-6Y, the preservation number is CGMCC No.32718, the strain is a gram-positive strain, the colony is milky white, nearly round, opaque, the surface is slightly rough, and has no luster. The Pseudomonas australis 94-6Y strain can significantly inhibit the water production breeding pathogenic bacteria Vibrio vulnificus, Vibrio splendidus and Vibrio alginolyticus; meanwhile, it is proved that the Pseudomonas australis 94-6Y strain has the ability of efficiently reducing ammonia nitrogen, nitrite nitrogen and chemical oxygen demand in breeding water, is helpful to reduce the breeding disease risk coefficient, improves the breeding water environment, and has important significance for strengthening the research and development of microecological preparation products and popularization of the ecological healthy breeding mode.

The application discloses a whole-genome SNP molecular marker combination related to the apraocha of sea cucumber against vibrio splendidus and application thereof, wherein the SNP molecular marker combination comprises 35 SNP sites, and in disease-resistant breeding, individuals with the 35 SNP site dominant genotypes can be screened to accelerate the breeding process of the disease-resistant traits. The molecular marker provided by the application has high biological correlation and prediction accuracy in breeding, and can be screened through DNA detection without relying on the phenotype of individual infection and disease, so that the generation interval is greatly shortened, the breeding efficiency is improved, the molecular marker assisted selection can overcome environmental interference, the genotypes are directly selected, the selection strength is high, the accuracy is high, and the genetic progress of the disease-resistant traits is accelerated.

The invention discloses an antibacterial peptide for preventing and treating black mouth disease of cultured sea urchin and application thereof, the amino acid sequence of the antibacterial peptide for preventing and treating black mouth disease of cultured sea urchin is VDTAHKGEVYPIGKAECPIP, and the antibacterial peptide can generate specific interaction with cell membrane of vibrio splendidus, which is the pathogenic bacterium of black mouth disease of strongylocentrotus intermedius, so that the integrity of the cell membrane of vibrio splendidus is destroyed, and the antibacterial peptide can be used for preventing and treating black mouth disease of cultured sea urchin. The minimum inhibitory concentration is 50 mg / mL, and the strain can be used as a feed additive for preparing a feed for culturing the sea urchin so as to prevent and treat the black mouth disease of the cultured sea urchin, and has a good application prospect.

The invention provides a method for improving vibrio splendidus infection resistance of stichopus japonicus, which is a method for preventing and treating stichopus japonicus diseases caused by vibrio splendidus by inhibiting pathogenesis related to vibrio splendidus outer membrane vesicles. The composition capable of improving the vibrio splendidus infection resistance of the stichopus japonicus comprises chlorpromazine and nystatin. The composition provided by the invention has no obvious toxicity to coelomic cells of the stichopus japonicus, and can inhibit endocytosis of OMVs to the maximum extent, so that the optimal prevention and control effect is realized. After the OMVs are treated by chlorpromazine and nystatin, the endocytosis efficiency of the OMVs is reduced by more than 50%.

The present application relates to aquaculture technology, in particular to a method for improving the growth and development speed and antibacterial ability of long oyster larvae by using taurine. The taurine soaking treatment of long oyster shell top larvae can promote the growth of larvae, accelerate the attachment metamorphosis process, and improve the survival rate after infection of Vibrio splendidus. The present application proves that taurine can improve the growth and development speed and immune defense ability of long oyster larvae, and has broad application prospects in shortening the oyster breeding cycle, improving the success rate and seed quality of breeding, promoting the green development of oyster culture industry, etc.

The invention provides a functional gene capable of maintaining immune memory of stichopus japonicus. The amino acid sequence of a protein coded by the functional gene is SEQ ID NO: 2. The gene is activated when the stichopus japonicus is infected by low-concentration vibrio splendidus, and can keep a relatively stable expression level; by regulating and controlling the metabolic pathway of the immune memory cells, the immune memory cells are converted to the glycolysis direction, so that rapid and sufficient energy support is provided for immune response; meanwhile, the gene can also effectively enhance the phagocytic function of coelomic cells of the stichopus japonicus, and obviously improve the ability of the stichopus japonicus to remove pathogenic microorganisms. Discovery and application of the gene provide a new molecular basis for research on a maintenance mechanism of immune memory of the stichopus japonicus, and lay a theoretical and practical foundation for prevention and control of stichopus japonicus diseases and development of a novel immune regulation method.

The application provides application of indole-3-lactic acid in improving immunity of sea cucumber and inhibiting virulence factors of Vibrio splendidus. The application is used for preparing a product for inhibiting virulence factors of Vibrio, and a product for improving immune defense ability of aquatic animals; wherein the aquatic animals are preferably sea cucumber, and the Vibrio is preferably Vibrio splendidus. Researches show that under the premise of basically not affecting the growth of Vibrio splendidus, the indole-3-lactic acid can significantly reduce hemolytic activity, protease activity, motility and biofilm formation of the Vibrio splendidus, and can reduce intestinal permeability of the sea cucumber, repair intestinal physical barrier, relieve excessive inflammatory response induced by Vibrio splendidus infection, thereby improving survival rate of the sea cucumber after attack and reducing incidence probability. The technology has the advantages of small dosage, clear effect, green safety and low drug resistance selection pressure, and is suitable for prevention and control of sea cucumber skin rot syndrome.

The present application provides a method and application of 5-iodoindole inhibiting virulence factors of Vibrio splendidus, and it is found that different concentrations of 5-iodoindole can significantly reduce the expression of virulence factors such as hemolytic activity and protease activity of Vibrio splendidus under the premise of basically not affecting the growth of Vibrio splendidus. Through the method, the infection toxicity of Vibrio splendidus can be weakened, so as to reduce the infection and disease probability of host sea cucumber and alleviate the infection symptoms. Meanwhile, the present application also provides the application of 5-iodoindole in reducing the expression of Vibrio splendidus related virulence genes and the application of 5-iodoindole in improving the survival rate of host sea cucumber after Vibrio splendidus attack, which proves that 5-iodoindole with a concentration of 20 muM has guiding significance for actual sea cucumber production and culture. And through the research on virulence factors of Vibrio splendidus, the present application is helpful to reduce the infection and disease of sea cucumber caused by Vibrio splendidus.