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Bacillus mojavensis and microorganism bacterium agent thereof for controlling cucumber downy mildew

A technology of Bacillus mogava and a microbial inoculum is applied in the field of application of preventing and treating downy mildew of cucumber, and can solve the problems that pathogenic bacteria are easy to produce drug resistance, difficult to apply, and high production cost

Active Publication Date: 2012-07-18
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are nearly 10 kinds of plant extracts used to prevent and control cucumber downy mildew. However, the extraction process of plant extracts is cumbersome, the production cost is high, and the pathogenic bacteria are prone to drug resistance after the use of artificially synthesized single substances, making this method difficult to apply.

Method used

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  • Bacillus mojavensis and microorganism bacterium agent thereof for controlling cucumber downy mildew
  • Bacillus mojavensis and microorganism bacterium agent thereof for controlling cucumber downy mildew
  • Bacillus mojavensis and microorganism bacterium agent thereof for controlling cucumber downy mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The preparation of HMB20199 microbial bacterial agent is carried out according to the following steps:

[0053] (1) Strain activation: The bacterial strain HMB20199 (Bacillus mojavensis) strain HMB20199 stored at -40°C has been preserved in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures on December 20, 2011. The number is CGMCC No.5617) on LB plate medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) at 30 ℃ , Pick a single colony on LB slant medium (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1g, agar powder 15g, water 1000mL) and cultivate at 30°C for 24~ After 36 hours, an activated bacterial strain was obtained;

[0054] (2) Preparation of seed liquid: make LB liquid medium according to the conventional method (its composition and weight ratio are: tryptone 1g, yeast extract 0.5g, sodium chloride 1...

Embodiment 2

[0059] Example 2 Comparative test of control effect of Bacillus mogaf HMB20199 on cucumber downy mildew

[0060] (1) Test drug:

[0061] (1) Microbial agent: the HMB20199 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0062] (2) Chemical agent: azoxystrobin suspension concentrate (250 g / L) (Syngenta Co., Ltd., UK); dilute 1000 times with water.

[0063] (3) Blank control: clear water.

[0064] (2) Test method: This test was carried out in the laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences in the winter of 2010. The cucumber downy mildew bacteria used in this experiment came from the Pesticide Application Technology Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences, and were prepared into sporangia (1 × 10 5 -1.5×10 5 individual / mL) suspension for inoculation in this test.

[0065] Get disease-free complete cucumber leaves, which are respectiv...

Embodiment 3

[0069] Example 3 Comparative Test of Control Effect of Bacillus mogaf HMB20199 on Cucumber Downy Mildew

[0070] (1) Test drug:

[0071](1) Microbial agent: the HMB20199 liquid preparation prepared in Example 1, diluted 50 times with water;

[0072] (2) Chemical agent: silver Farley suspension concentrate (687.5 g / liter) (Germany Bayer Crop Science Company), diluted 800 times with water;

[0073] (3) Blank control: clear water.

[0074] (2) The time and place of the experiment: in December 2010, it was carried out in the artificial climate chamber of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0075] (3) Test method: screen plump cucumber seeds, sow them in plastic flower pots with a diameter of 20 cm when they are accelerated to the point of dew, 15 pots per treatment (2 plants per pot), repeat 3 times. Arranged in random groups. Get the cucumber seedlings that grow to 3-4 true leaves and have the same growth potential, and spr...

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Abstract

The invention discloses a Bacillus mojavensis strain HMB20199 for controlling cucumber downy mildew, wherein the Bacillus mojavensis strain HMB20199 is preserved in China Microbial Culture Collection Management Center on December 20th, 2011, with the preservation number of CGMCC No.5617. The invention also discloses a microorganism bacterium agent produced by using the HMB20199 and a preparation method of the microorganism bacterium agent. Active components of the microorganism bacterium agent are the Bacillus mojavensis HMB20199 thallus and extracellular metabolites thereof. The HMB20199 strain disclosed by the invention is strong in specificity in controlling of the cucumber downy mildew and high in control efficiency, wherein the average control efficiency is over 85 percent; and moreover, the drug resistance is not easily generated by controlling the cucumber downy mildew through the Bacillus mojavensis strain HMB20199. In addition, the microorganism bacterium agent has no harm topeople or livestock and does not pollute the environment. The preparation method of the microorganism bacterium agent is simple, low in cost and convenient in use.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to a bacillus mogaffus for preventing and treating cucumber downy mildew, a microbial agent containing the bacillus mogaffus, and their application in preventing and treating cucumber downy mildew. Background technique [0002] Cucumber downy mildew is an infectious airborne disease caused by Pseudoperonospora cubensis Rostow, which poses a huge threat to cucumber production. The methods of controlling cucumber downy mildew mainly include disease-resistant breeding, chemical control and ecological control (such as high-temperature stuffy shed, etc.). Because cucumber downy mildew is a quantitative trait genetically controlled by multiple genes, it is difficult to carry out disease-resistant breeding and the resistance is easy to lose; chemical control has the problems of easily producing drug resistance and polluting people and the environment; and the high-tem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
Inventor 郭庆港马平李社增鹿秀云李宝庆
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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