121 results about "Human skin fibroblast" patented technology

This invention relates to compositions comprising an ethanolamine derivative and an organic salt of zinc and/or magnesium. It further relates to the use of such compositions in topical formulations, in particular in anti-aging formulations. The invention further relates to the use of such compositions to promote human dermal fibroblasts growth and to combat the effects of skin aging.

The present invention describes therapeutic compositions comprising one or more minerals, including trivalent iron, divalent manganese and salts thereof, suitable in facilitating synthesis and deposition of connective tissue matrix, particularly rich of elastin and collagen, and mitogenic potential in human dermal fibroblasts. It also describes the phenomenon in which stimulation of elastogenesis by arterial SMC associates with a net decrease in proliferation of these cell types. The present invention also describes methods of treatment of human skin fibroblasts and arterial smooth muscle cells. The therapeutic compositions of the present invention comprise one or more of trivalent iron or divalent manganese or salts thereof and may be combined with an elastic tissue digest.

The invention discloses an extraction method and application for a ganoderma lucidum spore active composition. The extraction method comprises the following steps: (1) performing softening processing, namely, immersing ganoderma lucidum spore in a mixed enzyme solution containing cellulase with the mass concentration of 0.2-0.5% and pectase with the mass concentration of 0.2-0.5%, so as to soften ganoderma lucidum spore wall; (2) performing low-temperature vacuum drying; and (3) performing low-temperature wall breaking, namely, performing mechanical crushing wall breaking on ganoderma lucidum spore at a low-temperature environment. The method combines a double-enzyme solution and a low-temperature mechanical process for softening ganoderma lucidum spore cell wall, and helps to effectively reduce difficulty for crushing ganoderma lucidum spore cell wall and shorten the time. The extracted ganoderma lucidum spore active composition is free of toxic and side effects on human skin fibroblast, is applicable to anti-ageing cosmetic products, such as facial masks, face cream, emulsion and other cosmetic, and effectively reaches the effects of removing acnes, eliminating pockmark, removing wrinkles, removing fine wrinkles and the like.

The invention discloses an umbilical cord mesenchymal stem cell exosome, a preparation method and an application of the exosome to a cosmetic. The exosome released by mesenchymal stem cells has the characteristics of the stem cells and has various biological functions of promoting tissue repair, immune suppression, nerve protection and the like. Although the mesenchymal stem cells are regarded asthe strongest cells generating the exosome, market requirements are still difficultly met when the exosome is used as a cosmetic raw material. The umbilical cord mesenchymal stem cell exosome has thefunction of promoting human skin fibroblast proliferation, the human skin fibroblast proliferation promoting function of the exosome can be remarkably improved after the mesenchymal stem cells are modified and transformed to highly express miR-525-3p, the skin aging resistance activity of unit mass of the exosome is remarkably improved, and the umbilical cord mesenchymal stem cell exosome and themiR-525-3p high-expression umbilical cord mesenchymal stem cell exosome can be used for the cosmetic resisting skin aging.

The invention discloses an earthworm extract having a skin aging resisting effect and a preparation method and application thereof. The earthworm extract contains polypeptides of which the molecular weight is less than or equal to 30000 Dalton, amino acid, proteins, lumbricin, vitamins and trace elements. The method comprises the following steps: smashing clean earthworms or smashing dry earthworms and soaking into water to obtain earthworm sample liquid; hydrolyzing the earthworm sample liquid by using own enzyme or added protease to obtain earthworm hydrolysate; performing ultrafiltration on the earthworm hydrolysate through an ultrafiltration membrane of which the molecular weight cutoff is less than or equal to 30000 Dalton, and freeze-drying to obtain the earthworm extract. By adopting the method, original fishy smell of earthworms is removed in the obtained earthworm extract. The earthworm extract has remarkable antioxidant activity, can be used for effectively promoting the growth and proliferation of human skin fibroblasts and promoting the synthesis of substrates such as collagen to achieve the effect of resisting aging, and can be applied to development of cosmetics and health products having the effects of resisting skin aging.

The invention relates to a method of inducing a human fibroblast to reprogram the human fibroblast into an osteoblast by utilization of Runx2 and a low-molecular-weight compound, particularly relates to a method of inducing a human fibroblast to transdifferentiate the human fibroblast into an osteoblast under the existence of dexamethasone by utilization of Runx2 gene expression, a low-molecular-weight compound CHIR99021 and/or a low-molecular-weight compound forskolin, and belongs to the fields of cytobiology and tissue engineering. The method includes: constructing a human skin fibroblast infected by a slow virus carrying Runx2-GFP, performing transdifferentiation of the human fibroblast under the existence of the dexamethasone by utilization of the low-molecular-weight compound CHIR99021 having a concentration of 5-10 muM and the low-molecular-weight compound forskolin having a concentration of 10 muM, changing into an osteoblast inducing liquid after 7-15 days, and inducing, and the fibroblast can be induced into the osteoblast after 5-20 days. The method can obtain osteoblasts simply and rapidly and can be used for cell therapy for bone metabolic disease, fracture, and the like. A pharmaceutical composition provided by the invention can be used for research, development and production of tissue-engineered bone, brings convenience to fundamental research and brings a prospect for clinical application.

The invention discloses a lumbricus peptide capable of resisting skin aging as well as a preparation method and an application thereof. The preparation method comprises the following steps: hydrolyzing an earthworm sample liquid by using own enzyme or adding protease, then carrying out membrane filtration and membrane interception on the hydrolysate, and freezing and drying to acquire the lumbricus peptide which can be applied to cosmetics or health care products capable of resisting skin aging. By the way, the preparation method is green and simple, and the prepared lumbricus peptide has the advantages of strong affinity, high safety, obvious antioxidant activity and capabilities of effectively promoting growth and multiplication of human skin fibroblast, significantly promoting secretion and synthesis of cell matrix collagen and obviously resisting skin aging and can be applied to the development of cosmetics and health care products capable of resisting skin aging.

The invention provides a method for inducing human skin fibroblasts to differentiate into adipocytes in vitro. The method comprises the following steps: A, carrying out in-vitro culture and passage onthe human skin fibroblasts in a basal culture medium, and carrying out induced differentiation when the cell confluence degree reaches 80-100%; and B, changing the basic culture medium in the step Ainto an induced differentiation culture medium (1), culturing for 6-10 days, and changing liquid once every two days; then, replacing the induced differentiation culture medium (1) with an induced differentiation culture medium (2), and culturing for 2-4 days; and finally, changing the induced differentiation culture medium (2) into a basic culture medium, culturing for 8-14 days, and changing liquid once every two days. According to the method, the problem that human skin fibroblasts are induced to be differentiated into adipocytes is successfully solved; the skin fibroblasts are induced to be differentiated into the adipocytes, so that scars left in skin during wound healing are reduced, and a new thought and a new therapy are provided for clinical wound repair, scar repair and the like.

The invention provides a skin-whitening toner containing earthworm extracting solution. The skin-whitening toner is characterized by being prepared from the following components by weight: 1.5-2.5 g of rhizoma smilacis glabrae extract, 4-6 g of wax gourd powder, 55-65 g of the earthworm extracting solution, 2-4 g of uronic acid, 1.5-2.5 g of an emulsifier, 1.5-2.5 g of a humectant, 1.5-2.5 g of white grape wine, 2-4 g of rhizoma dioscoreae juice, 4-6 g of radix sophorae flavescentis gel, 0.5-1.5 g of glycerol, 1.5-2.5 g of Vaseline, 2-4 g of lotus root starch, 0.4-0.6 g of protein zinc, 1.5-2.5 g of esterifying enzyme compound bacteria solution, and 30-50 g of purified water. The skin-whitening toner contains polypeptide, amino acid, protein, lumbricin, vitamin and various microelements, has the obvious antioxidant activity, can effectively promote the growth and proliferation of human skin fibroblasts, and promote the synthesis of the matrix such as collagen, and finally, the purposes of whitening the skin and resisting ageing are achieved.

The invention relates to the field of chemical engineering in a hospital, particularly relates to a new application of a small molecule compound, and further particularly relates to an application of a small molecule compound danshinolic acid B in preparation of a medicine of treating scleroderma. The invention provides an application of danshinolic acid B in preparation of a medicine of treating scleroderma, and further provides a corresponding medicine composition and a medicine preparation. The inventor of the invention makes an animal experiment, which shows that a compound SAB remarkably relieves the pulmonary fibrosis of a mice suffering from the scleroderma, and a further cell experiment indicates that the small molecule compound can inhibit cell inflammation induced by TNFalpha, and can inhibit the skin flbroblast of a patient suffering from the scleroderma cultured in vitro, human embryonic lung fibroblast proliferation and collagen expression, and inhibit transformation of pulmonary epithelial cells to mesenchymal cells. The application shows that the compound has a function of resisting fibrosis of the scleroderma, and has a good application prospect for preventing and treating the scleroderma.

The invention discloses a reprogramming culture medium, a preparation method thereof and a culture method of a reprogramming cell, wherein the reprogramming culture medium comprises a basal culture medium, a reprogramming additive 1 and a reprogramming additive 2, wherein the basal culture medium is a DMEM (Dulbecco Modified Eagle Medium)/F12 culture medium; the reprogramming additive 1 comprises sodium hydrogen carbonate, sodium selenate, recombinant human insulin, recombinant human basic fibroblast growth factors, recombinant human transferrin, ascorbic acid and hydrocortisone; the reprogramming additive 2 comprises sodium hydrogen carbonate, sodium selenate, recombinant human insulin, recombinant human basic fibroblast growth factors, recombinant human transferrin, ascorbic acid, valproic acid and sodium butyrate. The reprogramming culture medium disclosed by the invention is exact in component, stable in quality, high in reprogramming efficiency, wide in cell lineage and capable of reprogramming and culturing cells such as human skin fibroblasts, peripheral blood mononuclear cells, amniotic epithelial cells and the like.

The invention relates to the field of exosome separation, in particular to a human skin fibroblast exosome preparation and separation method, which specifically comprises the following steps: S1, performing multiplication culture on human skin fibroblasts; s2, replacing a serum-free culture medium, performing starvation culture on the amplified human skin fibroblasts, switching to an oxygen-free state in the starvation culture process, and introducing a small amount of nitric oxide to stimulate the human skin fibroblasts; s3, collecting the supernate, and filtering the supernate through a filter membrane to remove cells and cell debris; and S4, separating the exosomes in the supernate through an ultracentrifugation method. In the application, the human skin fibroblasts are stimulated by nitric oxide, so that the human skin fibroblasts can secrete the exosomes better, and the yield of the exosomes is further improved.

The invention belongs to the field of cosmetics and particularly relates to an anti-aging composition and preparation and application thereof. The anti-aging composition is prepared from the following ingredients in parts by weight: 1 to 3 parts of hydrolyzed pearl powder, 1 to 3 parts of dioscorea villosa root extract, 0.5 to 2 parts of acetyl hexapeptide-8, 0.5 to 2 parts of acetyl octapeptide-3 and 0.5 to 2 parts of nonapeptide-1. Experiments prove that the anti-aging composition disclosed by the invention has an extremely obvious removing effect on quiescent wrinkles, dynamic wrinkles and posture extrusion wrinkles, meanwhile can obviously promote multiplication of human skin fibroblast, extremely obviously promotes synthesis of I and III type collagen and extremely obviously inhibits expression of MMP1 and MMP3.

The invention discloses a human skin fibroblast extraction method. The method includes the steps of: 1) cutting human dermal tissue into pieces and conducting digestion with a mixed enzyme solution containing 0.25% IV type collagenase and 0.1% trypsin until large tissue blocks disappear, and then stopping digestion; 2) filtering the digested dermal tissue, collecting the filtrate, performing centrifugation, discarding the supernatant, collecting precipitated cells and inoculating the cells in a culture bottle, and performing culture with a screening medium; 3) conducting culture till a cell fusion degree up to 70%, then replacing part of the screening medium as an amplification medium, and further conducting culture; and 4) after culture to a cell fusion degree up to 90%, conducting subcultring, and replacing all the medium as the amplification medium. Specifically, the formula of the screening medium adopts a DMEM high sugar medium as the basic medium, which contains fetal bovine serum with a volume content of 8-12%, and 0.4-0.6ng/mL EGF and 4-6ng/mL bFGF as adding factors; and the formula of the amplification medium adopts the DMEM high sugar medium as the basic medium, which contains fetal bovine serum with a volume content of 8-12%.

The invention discloses a preservative-free polypeptide composition with an anti-aging effect. The preservative-free polypeptide composition is freeze-dried powder prepared from the following raw materials in percentage by mass of 1%-20% of compound polypeptide, 1%-20% of recombinant human I-type collagen and 60%-98% of a freeze-drying protective agent, wherein the compound polypeptide comprises the following components in percentage by mass of 15%-30% of oligopeptide-1, 20%-30% of carnosine, 5%-30% of tripeptide-1 and 10%-55% of pentapeptide-3. The polypeptide composition has a remarkable proliferation promoting effect on human skin fibroblasts, has a promoting effect on I-type collagen gene expression at high concentration has a promoting effect on III-type collagen gene expression at low concentration, and keeps the activity of peptide raw materials to the maximum extent; and meanwhile, the polypeptide composition does not contain preservative, so that the influence of the preservative on the activity and stability of the peptide components is avoided. The polypeptide composition disclosed by the invention can be added into different products such as water aqua, cream, masks andessences according to requirements, so that the anti-aging effect is provided for the products.

The invention relates to skin tissue engineering seed cells, a construction method, an adenovirus vector and a use thereof, and the construction method comprises the steps of culturing human skin fibroblasts and constructing the adenovirus vector containing double genes of CTLA4Ig-CD40Ig; and the in vitro transfection of the human skin fibroblasts is carried out by the adenovirus vector containing the double genes of CTLA4Ig-CD40Ig. The skin tissue engineering seed cells with low immunogenicity are produced by the above construction method. The adenovirus vector used for transfection of the human skin fibroblasts is the Ad5F35 chimeric adenovirus vector which contains CTLA4Ig gene and CD40Ig gene effectively connected with a promoter. The invention relates to the use of adopting the adenovirus vector in the preparation of the transfected human skin fibroblasts for repairing injured skin. The invention can effectively reduce the immunogenicity of the seed cells, and the transfected human skin fibroblasts do not affect the proliferation performance thereof in vitro.