Application of danshinolic acid B in preparation of medicine of treating scleroderma
A technology of scleroderma and salvianolic acid, applied in skin diseases, drug combinations, antipyretics, etc., can solve problems such as difficulty in preventing disease progression, tissue damage, large side effects, and poor efficacy
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Embodiment 1
[0050] The compound salvianolic acid B used in the present invention is obtained through commercial channels.
[0051] Compound salvianolic acid B alleviates lung fibrosis in scleroderma mice:
[0052] Test objects: 7-week-old C57BL / 6 female mice, purchased from the Experimental Animal Center of Fudan University
[0053] experiment method:
[0054] 1) Establishment of scleroderma pulmonary fibrosis mouse model: Bleomycin-induced scleroderma mouse model is a mature and well-recognized tool for studying fibrosis by scientists from all over the world. Intubation of bleomycin into the lungs can cause pulmonary fibrosis. Seven-week-old C57BL / 6 female mice were used to establish a model of bleomycin (BLM)-induced pulmonary fibrosis in scleroderma by endotracheal intubation. The dosage of bleomycin was changed to 9mg / kg, given once. [Note: The mouse culture time is as described in 2)]
[0055] 2) The effect of compound SAB treatment on scleroderma fibrosis in mice: divided into ...
Embodiment 2
[0061] The compound salvianolic acid B inhibits the occurrence of TNFα-induced cellular inflammation:
[0062] Test objects: NIH3T3 cells (mouse embryonic fibroblasts), MRC5 cells (human embryonic lung fibroblasts) Test methods:
[0063] 1) Applying TNFα to stimulate cells to establish an in vitro inflammatory cell model (Zhu X, Liu Q, Wang M, Liang M, Yang X, Xu X, Zou H, Qiu J. Activation of Sirt1by resveratrol inhibits TNF-α induced inflammation in fibroblasts.PLoS One .2011;6(11):e27081), the expression levels of IL-1β, IL-6, COX-2, iNOS and other inflammatory factors at different time points of TNFα were detected by Real-time PCR;
[0064]2) Obtain cells from different treatment groups of SAB or TNFα. For SAB and TNFα treatment groups simultaneously, NIH3T3 cells were cultured with DMEM medium, MRC5 cells were cultured with MEM medium, and about 8*105 (10 of 5 After 24 hours of culture, add different concentrations of SAB into the cell culture medium (final concentration...
Embodiment 3
[0069] The compound salvianolic acid B exerts anti-fibrotic effects in fibroblasts:
[0070] Test objects: MRC5 cells (human embryonic lung fibroblasts), primary cultured skin fibroblasts from patients with scleroderma Test methods:
[0071] 1) The cell dynamics model analysis system based on xCELLIgence technology to study the effect of SAB on the proliferation of fibroblasts. Add 1000 cells to the wells of each E-Plates plate. After culturing for 24 hours, replace the culture medium without or containing 100ug / ml SAB into the cells, and observe the cell proliferation curve in real time. The larger the cell index, the more cells there are. [Description: The xCELLigence system is a non-labeled real-time cell analysis system launched by Roche, which uses bioelectrical resistance readings to non-subjectively quantify the cell state in real time. The system monitors cell activity in real time without adding labels. The system measures electrical impedance on interdigitated micr...
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