Method for preparing autologous triple skin fibroblast preparation

A technology of fibroblasts and fibrogenesis, which is used in skin care preparations, medical preparations containing active ingredients, prostheses, etc.

Inactive Publication Date: 2017-11-21
四川驰鼎盛通生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing an autologous triple skin fibroblast preparation, aiming at solving the problem of a safe, effective and long-lasting individualized preparation of fibroblasts

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  • Method for preparing autologous triple skin fibroblast preparation

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Embodiment Construction

[0044] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0045] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0046] The autologous triple skin fibroblast preparation provided by the embodiment of the present invention is mixed with the platelet-rich plasma PRP and the autologous collagen concentrate at a ratio of 1:4, and the fibroblasts are added as the base liquid of the preparation.

[0047] Such as figure 1 As shown, the preparation method of the autologous triple skin fibroblast preparation provided by the embodiment of the present invention comprises the following steps:

[0048] S101: ...

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Abstract

The invention discloses a method for preparing an autologous triple skin fibroblast preparation. The autologous triple skin fibroblast preparation is composed of platelet-rich plasma, autologous collagen concentrate and fibroblasts; platelet-rich plasma: autologous collagen concentrate= 1:4, the concentration of autologous fibroblasts is (1.0~2.0)×107 / ml. The preparation method of the autologous PRP skin fibroblast preparation comprises the following steps: separation and cultivation of human skin fibroblasts; preparation of autologous high-concentration collagen liquid; preparation of autologous platelet-enriched plasma; autologous triple skin fibroblast injection preparation. The active ingredients of the preparation of the present invention all belong to the client's own body, and in the process of culturing the fibroblasts, the autologous serum medium is used for culturing, which truly achieves the characteristics of safety, effectiveness and individualization. It can be used for skin wrinkle removal, scar repair, regeneration, etc., to solve skin aging problems.

Description

technical field [0001] The invention belongs to the technical field of anti-aging beauty products, and in particular relates to a preparation method of an autologous triple skin fibroblast preparation. Background technique [0002] A few days ago, there are already various beauty products on the market. Botulinum endotoxin, as the current mainstream beauty product, is popular because of its ease of use, remarkable effect, safety and reliability. However, there are many side effects after their use, such as unnatural expression, photophobia, tearing, local swelling, ecchymosis, numbness at the injection site, headache, tightness in the forehead, and deepening of wrinkles in adjacent parts. The application of these two factors tends to produce obvious dependence. As the number of injections increases, people's sensitivity to them decreases, causing patients to use this method more frequently, and the amount used each time will gradually increase. In the process of skin aging,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/50A61L27/60A61K8/99A61K8/98A61K8/65A61Q19/00A61Q19/08
CPCA61L27/3804A61K8/65A61K8/983A61K8/985A61K8/99A61K2800/805A61K2800/91A61L27/24A61L27/3616A61L27/3895A61L27/50A61L27/60A61L2400/06A61Q19/00A61Q19/08
Inventor 胡火珍宋丽萍杨燕肖春谢小琴王曦李利张泽辛张娟罗旭梅彭芳芳薛红刘佩娜叶林胡章兰黄娟邹琳
Owner 四川驰鼎盛通生物科技有限公司
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